2011
DOI: 10.1016/j.cell.2011.03.031
|View full text |Cite
|
Sign up to set email alerts
|

Induced Ectopic Kinetochore Assembly Bypasses the Requirement for CENP-A Nucleosomes

Abstract: Summary Accurate chromosome segregation requires assembly of the multi-protein kinetochore complex at centromeres. Although prior work identified the centromeric histone H3-variant CENP-A as the important upstream factor necessary for centromere specification, in human cells CENP-A is not sufficient for kinetochore assembly. Here, we demonstrate that two constitutive DNA-binding kinetochore components, CENP-C and CENP-T, function to direct kinetochore formation. Replacing the DNA-binding regions of CENP-C and … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

34
471
2
2

Year Published

2012
2012
2017
2017

Publication Types

Select...
6
2
1

Relationship

0
9

Authors

Journals

citations
Cited by 324 publications
(521 citation statements)
references
References 39 publications
34
471
2
2
Order By: Relevance
“…4A). This functional analysis was supported by staining for CENP-C -a crucial kinetochore protein, which links the inner and outer kinetochore components (Saitoh et al, 1992;Tomkiel et al, 1994;Gascoigne et al, 2011;Przewloka et al, 2011;Screpanti et al, 2011). CENP-C was severely depleted from the HAC kinetochore following the tethering of tetR-EYFP-VP16, but could be detected at HAC kinetochores in the presence of tetR-EYFP-p65 and the control, tetR-EYFP (Fig.…”
Section: Acetylated Chromatin Is Compatible With the Integrity Of Thementioning
confidence: 79%
“…4A). This functional analysis was supported by staining for CENP-C -a crucial kinetochore protein, which links the inner and outer kinetochore components (Saitoh et al, 1992;Tomkiel et al, 1994;Gascoigne et al, 2011;Przewloka et al, 2011;Screpanti et al, 2011). CENP-C was severely depleted from the HAC kinetochore following the tethering of tetR-EYFP-VP16, but could be detected at HAC kinetochores in the presence of tetR-EYFP-p65 and the control, tetR-EYFP (Fig.…”
Section: Acetylated Chromatin Is Compatible With the Integrity Of Thementioning
confidence: 79%
“…[17][18][19] CENP-C and CENP-T serve as a platform for recruitment of the KMN network through pathways that are not yet well understood. [20][21][22][23][24][25][26][27][28][29] From the CCAN components, CENP-N and CENP-C recognize CENP-A. [30][31][32] Depletion of CENP-N or CENP-C in human cells by RNA interference leads to decreased incorporation of new CENP-A at centromeres.…”
Section: Introductionmentioning
confidence: 99%
“…CENP-T is a component of the constitutive centromere-associated network (CCAN), which plays a central role in kinetochore assembly, mitotic progression, and segregation of chromosomes [23]. CENP-T has been identified as substrate of Haspin by consensus site prediction (see below), and its phosphorylation on several sites confirmed by in vitro kinase assay [11].…”
Section: Haspin Substratesmentioning
confidence: 99%