Induced genome maintenance pathways in pre-cancer tissues describe an anti-cancer barrier in tumor development

Simão, Éder Maiquel; Sinigaglia, Marialva; Bugs, Cristhian Augusto; Castro, Mauro A. A.; Librelotto, Giovani Rubert; Alves, Ronnie; Mombach, José C. M.

doi:10.1039/c2mb25242b

Cited by 11 publications

(14 citation statements)

References 36 publications

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“…Expression of three target genes (H2AFX, RUNX3 and MTHFD1L) of miR-133a and one target gene ( FYB ) of miR-206 was upregulated while expression of these two miRNAs ( miR-133a and miR-206) were significantly downregulated in cancer tissues (Table 4). H2AFX is one of the histone family member gene which are involved in DNA double strand break repair pathway23. Although methylation, copy number alterations, deletion and single nucleotide polymorphisms in H2AFX are well-linked with different types of cancer, expression deregulation of the gene is also reported in a few studies24252627.…”

Section: Discussionmentioning

confidence: 99%

Expression deregulation of mir31 and CXCL12 in two types of oral precancers and cancer: importance in progression of precancer and cancer

Chattopadhyay

Ray

Roy

et al. 2016

Sci Rep

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Oral cancer generally progresses from precancerous lesions such as leukoplakia (LK), lichen planus (LP) and oral submucous fibrosis (OSMF). Since few of these precancers progress to cancers; it is worth to identify biological molecules that may play important roles in progression. Here, expression deregulation of 7 miRNAs (mir204, mir31, mir31*, mir133a, mir7, mir206 and mir1293) and their possible target genes in 23 cancers, 18 LK, 12 LP, 23 OSMF tissues compared to 20 healthy tissues was determined by qPCR method. Expression of mir7, mir31, mir31* and mir1293 was upregulated and that of mir133a, mir204 and mir206 was downregulated in cancer. Expression of most of these miRNAs was also upregulated in LK and LP tissues but not in OSMF. Expression deregulation of some of the target genes was also determined in cancer, LK and LP tissues. Significant upregulation of mir31 and downregulation of its target gene, CXCL12, in cancer, LK and LP tissues suggest their importance in progression of precancer to cancer. Expression upregulation of mir31 was also validated using GEO data sets. Although sample size is low, novelty of this work lies in studying expression deregulation of miRNAs and target genes in oral cancer and three types of precancerous lesions.

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Section: Discussionmentioning

confidence: 99%

Expression deregulation of mir31 and CXCL12 in two types of oral precancers and cancer: importance in progression of precancer and cancer

Chattopadhyay

Ray

Roy

et al. 2016

Sci Rep

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“…In all these instances, DDR checkpoint activation was accompanied by evidence of DNA double-strand breaks, as assessed by γH2AX expression. In addition, DDR has been observed in pre-cancerous lesions prior to the onset of the genomic instability that characterizes invasive cancer, indicating that widespread allelic imbalances were not the underlying basis for checkpoint activation within the epithelium (19). …”

Section: Discussionmentioning

confidence: 99%

Expression of the γ-phosphorylated histone H2AX in gastric carcinoma and gastric precancerous lesions

Guo

Pei

Si³

et al. 2015

Oncology Letters

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The histone γH2AX is a marker of activated DNA damage that is overexpressed in various cancers and corresponding precursor lesions, indicating that γH2AX is a component in oncogenic transformation. The present study aimed to determine whether the immunohistochemical expression of γH2AX is involved in the progression between superficial gastritis (n=20), atrophic gastritis (n=24) and gastric carcinoma (n=79). There was no increase in γH2AX expression between superficial and atrophic gastritis, but there was a significant increase in γH2AX expression between these two conditions and gastric carcinoma (χ2=68.712; P<0.001). The expression of γH2AX in moderately-differentiated gastric adenocarcinoma (n=49) was evidently higher compared with poorly-differentiated gastric adenocarcinoma (n=26; χ2=14.241; P<0.01). Staining for γH2AX did not reveal a significant association between the expression of the histone and patient age, depth of invasion, lymph node metastasis or the tumor-node-metastasis stage of the gastric carcinoma. Overall, the present study demonstrated that enhanced γH2AX expression may be closely associated with gastric carcinoma, but is less likely to be involved in the genesis of gastric carcinoma.

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“…where, and are the activity of the α pathway associated with experiment and control, respectively 20 , in which nα values are restricted between . For a group of genes, relative 0 ≤ ≤ 1 diversity was used to compare data distribution associated with the pathway in relation to the control, through the following relation:…”

Section: Relative Activity and Diversity Calculationmentioning

confidence: 99%

The response of genes and pathways of immunological system induced by irradiation

Ribeiro

Silveira

Simão

et al. 2019

Preprint

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Current studies have shown that ionizing radiation (IR) could increase the efficiency of radiation therapy by the stimulation of the immune system. This occurs in low-dose radiation as well as doses within hypofractionated range usually used in radiotherapy. However, the elucidation of the mechanisms of immunogenic modulation reported at these doses remain an issue. In this study, we analyzed transcriptome data available in Gene Expression Omnibus (GEO) database related to B cells isolated from whole blood of 95 donors and then irradiated with 10 Gy. The aim of this study is to investigate the regulation of genes and pathways of the immune system considering the B7-CD28/CTLA4 superfamily, CD40-CD40LG molecules, and cytokines expressed by B cells irradiated. The connection between genes and pathways is established by the Reactome database. Relative activity and diversity of pathways were calculated to determine the modulation of the immune system response to irradiation. Analysis of variance (ANOVA) with repeated measures and Bonferroni's method were used to determine differentially expressed genes. It was observed that IR up-modulates the response of pathways and genes considered in this study, which indicates that 10 Gy can enhance antitumor immune responses.Acknowledgments: This research was partially supported by CAPES. This work is based in a MSc thesis part submitted for the partial fulfillment of the requirements of the graduate programme in physics at the Federal University of Rio Grande, Rio Grande, Rio Grande do Sul, Brazil.

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Induced genome maintenance pathways in pre-cancer tissues describe an anti-cancer barrier in tumor development

Cited by 11 publications

References 36 publications

Expression deregulation of mir31 and CXCL12 in two types of oral precancers and cancer: importance in progression of precancer and cancer

Expression deregulation of mir31 and CXCL12 in two types of oral precancers and cancer: importance in progression of precancer and cancer

Expression of the γ-phosphorylated histone H2AX in gastric carcinoma and gastric precancerous lesions

The response of genes and pathways of immunological system induced by irradiation

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