Induced protein polymorphisms and nutritional quality of gamma irradiation mutants of sorghum

Mehlo, Luke; Mbambo, Zodwa; Bado, Souleymane; Lin, Johnson; Moagi, Sydwell M.; Buthelezi, Sindisiwe; Stoychev, Stoyan; Chikwamba, Rachel

doi:10.1016/j.mrfmmm.2013.05.002

Cited by 31 publications

(17 citation statements)

References 35 publications

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“…Nonetheless, in conformity with the results reported by Laskar et al 27 on Lens, we were able to make use of SDS-PAGE as a tool for determining genetic relationships within a mutagenized population of Vigna unguiculata. The variations in the electrophoresis patterns of the seed proteins after mutagen treatments have also been reported in various pulse crops [35][36][37][38][39] . Hong et al 40 advocated that the response of mutants towards a wide range of environmental stresses can also be determined using SDS-PAGE analysis and would be useful in screening the stress tolerant mutants with high yield.…”

Section: Seed Protein and Mineral Element Contentsmentioning

confidence: 74%

Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Raina

Laskar

Tantray

et al. 2020

Sci Rep

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cowpea, Vigna unguiculata (L.) Walp. is an important grain legume grown in the dry agro-ecologies of the tropics with considerably low yield due to lack of improved varieties, aggravated by prevalent narrow genetic base. thus, induced mutagenesis was employed using sodium azide and gamma rays to increase genetic variability in cowpea genotypes that resulted in isolation of eleven high yielding mutant lines at the M 4 generation from the genetic background of cowpea varieties Gomati VU-89 and Pusa-578. In order to analyze the induced genetic divergence among the mutant lines and parent genotypes, biochemical and molecular characterization was carried out with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), simple sequence repeat (SSR) and cAAt box derived polymorphism (cBDp) markers. Activity of nitrate reductase (nR) and content of chlorophyll, carotenoid, protein and mineral were found to be significantly high in the selected mutant lines compared to their respective parent genotypes. SDS-PAGE profile of seed proteins generated 54 and 28 polymorphic bands and a total polymorphism of 62.06 and 41.17% in Gomati VU-89 and Pusa-578, respectively. SSR primers amplified a total of 16 and 24 polymorphic bands with an average polymorphism of 20.69 and 50.74% in Gomati VU-89 and Pusa-578, respectively. CBDP markers, used for the first time in mutagenized population, generated 175 bands with 77 bands being polymorphic in Gomati VU-89 and 121 bands with 59 bands being polymorphic in Pusa-578. Physiological, biochemical and molecular profiling of the selected promising mutants lines showed that Gomati VU-89-G and Pusa-578-C are genetically most diverged high yielding genotypes with significant increase in protein and micronutrient content, therefore, could be recommended for further research considerations. thus, the favorable combination of genes induced in the novel cowpea mutants selected through the present study are valuable to correspond farmers requirements for new improved cultivars (direct or hybrids).Pulses are the chief components of the agricultural system, effectively boosting food and nutrition, revenue and environment across the globe and hence assumed ideal for acquiring food security in the developing world including India. Even though India is the leading producer of pulses with 26% of the total world production harvested at about 35% of the total world area, yet a prominent yield gap of 18% exists and one of the leading importer nation 1 . Cowpea [Vigna unguiculata (L.) Walp.] is a vital leguminous crop growing in the semi-arid tropics of Asia, Africa, Southern Europe, Southern United States, and Central and South America 2 . Cowpea leaves, green pods, and grains are excellent dietary source of protein, thus used as food and feed for healthy growth of both humans and livestock 3 . Cowpea is reportedly considered to be first originated and domesticated in Southern Africa, and later spread to east and West Africa and Asia 4 . Worldwide about 6.5 million metric tons of cowpea are produc...

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Section: Seed Protein and Mineral Element Contentsmentioning

confidence: 74%

Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Raina

Laskar

Tantray

et al. 2020

Sci Rep

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“…On the other hand, most of the remaining protein did not change significantly. Mehlo et al [21] reported that albumin and globulin content of sorghum flour decreased significantly even with low doses of irradiation which helps the protein to aggregate and might decrease its solubility.…”

Section: Resultsmentioning

confidence: 99%

Low gamma irradiation effects on protein profile, solubility, oxidation, scavenger ability and bioavailability of essential minerals in black and yellow Indian soybean (Glycine max L.) varieties

Krishnan

Singh

Thimmegowda

et al. 2015

J Radioanal Nucl Chem

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Effect of low doses of gamma irradiation (0.25, 0.5 and 1.0 kGy) on protein oxidation, profile, solubility, ROS scavenging and in vivo bioavailability of minerals in black (BS1) and yellow (BRAGG) soybean varieties were investigated. Increased oxidation, altered protein profile with decreased solubility was observed higher in BRAGG compared with BS1. The most significant ROS scavenging effect, antioxidant activity, least phytate content and improved bioavailability was found at 0.5 kGy in BS1 than BRAGG due to anthocyanins, and phenolics. Still 1.0 kGy is considered as toxicologically and microbiologically safe but it causes biochemical alterations and thus 0.5 kGy can be the optimum dose with enriched nutraceutical properties.

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“…Our study primarily focused on investigating the distribution, accumulation patterns and localisation profiles of a selected group of macro-and microelements in a mutant population of sorghum generated through gamma irradiation. We previously reported that this mutant population displayed protein polymorphisms and significant changes in protein and amino acid profiles beneficial to human and animal health (Mehlo et al, 2013).…”

Section: Introductionmentioning

confidence: 92%

“…Even though very little is known of the molecular mechanisms and the biology of accumulation of elements in plants, several methods have been employed to alter the accumulation dynamics, concentrations, and forms of elements in plants and their quantification. Making use of proton induced X-ray emission (PIXE) and electron microscopy, alterations in the profiles and elemental distribution of 9 elements, and the protein-and starch-body ultra-structure of gamma-irradiation mutants of sorghum have been resolved (Mehlo et al, 2013). These results prove that induced mutations can be a useful tool, in addition to other techniques like genetic engineering and breeding for traits associated with elemental accumulation.…”

Section: Introductionmentioning

confidence: 99%

8. Proton induced X-ray emission and electron microscopy analysis of induced mutants of sorghum

Mbambo¹,

Minnis‐Ndimba²,

Pineda³

et al. 2014

Mutagenesis: Exploring Novel Genes and Pathways

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Gamma irradiation induced alterations in the spatial distribution and localisation profiles of Fe, Zn, K, P, Cl, Ca, S, Mn, and Cu in mutant seeds of sorghum were determined using PIXE. The changes included enhanced or diminished accumulation of elements in preferential accumulation tissues and entire changes in cellular localisation. Transmission and scanning electron microscopy of the mutants resolved changes in size, shape, ultra-structure and packed cell volumes of proteinand starch bodies. The combined data suggest that induced mutations are an effective tool suitable for simultaneously targeting changes in multiple agronomic and nutritional traits that are crucial for human and animal health in important crops.

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Induced protein polymorphisms and nutritional quality of gamma irradiation mutants of sorghum

Cited by 31 publications

References 35 publications

Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Low gamma irradiation effects on protein profile, solubility, oxidation, scavenger ability and bioavailability of essential minerals in black and yellow Indian soybean (Glycine max L.) varieties

8. Proton induced X-ray emission and electron microscopy analysis of induced mutants of sorghum

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