Induced Stress on Red Blood Cell Promotes Red Blood Cell-Endothelial Adhesion

Mahalakshmi, Vijayaraghavan; Sengupta, Prattusha; Sumantran, Venil N.; Suganya, Natarajan; Chatterjee, Suvro

doi:10.1134/s1990519x20060085

Cited by 1 publication

(2 citation statements)

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“…Previously we had reported that cholesterol-depleted RBC adheres to EC pertaining to stress caused by the removal of cholesterol in RBC (30). In our present study, we observed similar RBC-EC adhesion abnormality when RBCs were treated with higher concentrations of band 3 inhibiting agent (DIDS).…”

Section: Sem Analysis Of Stressed Rbcs and Recovery By 8 Bromo-cgmpsupporting

confidence: 79%

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Nitric oxide donor 8-bromo-cGMP suppresses cholesterol depleted RBCs band 3 dysfunction

Vijayaraghavan¹,

Sengupta²,

Chatterjee³

et al. 2022

Cell Mol Biol (Noisy-le-grand)

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Red blood cells (RBCs) carry large cholesterol fractions and imbalance in them leads to several vascular complications. RBCs band 3 protein plays an important role in maintaining membrane integrity and there are many reports on cholesterol and band 3 protein interaction. Yet, RBCs band 3 protein role in regulating cholesterol homeostasis needs to be investigated. In this study, we induced cholesterol-depletion and band 3 inhibition in RBCs; both of which cause stress by decreasing band 3 channel activity with an increase in RBCs adhesion to endothelial cells (EC) by elevating band 3 phosphorylation (Tyr21), methemoglobin level and decreasing nitric oxide level. We hypothesized that nitric oxide (NO), a prominent determinant for RBC structural stability, would protect RBCs from stressors. To estimate this, we used three NO donors (SpNO, Sildenafil citrate and 8-Bromo-cGMP) and found that all 3 NO donors were able to recover, with 8-Bromo-cGMP being the most effective as it not only increased band 3 channel activity but also decreased RBC-EC adhesiveness and methemoglobin level in both stressors. Whereas NO donor's treatment did not display an ameliorative impact when both stresses were combined. Overall, these findings may shed light on the role of 8-bromo-cGMP in regulating RBC cholesterol homeostasis by maintaining band 3 function. Further studies in this direction might help identify targets for the therapeutic use of NO donors in the treatment of blood disorders.

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Section: Sem Analysis Of Stressed Rbcs and Recovery By 8 Bromo-cgmpsupporting

confidence: 79%

“…In several disease severity cases; RBCs were observed to be adherent to the endothelial cells (27,30). The chemicals used to inhibit band 3 demonstrated its ability to promote RBCs adhesion to EC under in vitro static conditions (Fig 3a).…”

Section: Attenuation Of Stressed Rbc Adhesion To Ec With No Donorsmentioning

confidence: 98%