Inducing oocyte maturation, ovulation and spawning in the African catfish, Heterobranchus longifilis Valenciennes (Pisces: Clariidae), using frog pituitary extract

Abstract

Abstract. Oocyte maturation, ovulation and spawning were successfully achieved in the African catfish, Heterobranchus longifilis Valenciennes, using frog pituitary extract. During artificial spawning, oocyte maturation and ovulation were induced at a dosage of 7mg per kg of individual fish weight. Latency period varied from 7 h at 29°C to 11 h at 25°C. Dead eggs became opaque between 8 and 10h after fertilization while embryogenesis was found to be temperature dependent. Mean hatching and larval deformity rates were 63·08%± 7·56 and 8·08%± 1·93 respectively. During induced natural spawning, the extract was applied at a dosage of 35 mg per kg of female fish weight. Spawning occurred between 10 and 11 h after hypophysation. Mean hatching and larval deformity rates were 41·38%± 8·48 and 10·00%± 2·00 respectively.

Role of Synthetic Hormones on Reproductive Performance in Etroplussuratensis

“…Nwokoye et al [16] stated that ovaprim recorded low deformities when compared with homoplastic hormone. In a similar study, Nwadukwe [19] using frog pituitary extract to induce breed H. longifilis reported a similar result to that of Nwokoye et al [16]. Studies of this present report and the earlier reports revealed that hormones have correlating effect on spawning parameters, as the most hormones are promoters or inhibitors, depending on dosage.…”

Induced propagation of African clariid catfish, Heterobranchus bidorsalis (Geoffrey Saint Hillarie, 1809) using synthetic and homoplastic hormones

“…Percentage deformity followed the same pattern -(0.12%) and (0.39%) respectively. In a similar study, Nwadukwe (1993) using frog pituitary extract to induce breed H. longifilis reported a survival rate of 66 -90% for hatchlings after one week. The slight difference in these results when compared with those in the present study may be attributed to species of fish and hormonal material used.…”

“…The percentage of dead eggs was (1.69%) for females injected with ovaprim and 3.99% for females injected with homoplastic hormone. In another study, Nwadukwe (1993) induced breeding H. longifilis with frog hormone recorded higher dead percentage (29%). The lower percentage recorded in the present study may be attributed to the efficacy of the hormones.…”

“…Richter and Van der Hurk (1982) reported that the problem of inadequate supply of fish seed can only be solved through induced breeding by the application of various inducement materials. Various types of fishes have been induced to spawn, using various hormonal materials (Nwadukwe, 1993;Eyo, 1997Eyo, , 1998Eyo, , 2000Nwuba and Aguigwo, 2002). Some of these hormonal materials (natural and synthetic) include cHCG (Eyo, 1997;1998), HCG (Eyo, 2002); clomipgene citrate (Aguigwo, 1991), pituitary extract (Janssen, 1985;Haniffa et al, 2000) and Ovaprim (Manosroi et al, 2004;AbolMunafi et al, 2006).…”

Evaluating the Efficacy of Pituitary Gland Extracts and Ovaprim in Induced Breeding and Fry Quality of Clarias gariepinus, Burchell (Pisces: Claridae)

“…Induced breeding involves the use of some exogenous ovulating agents, which trigger the ripening of mature eggs (Ajah, 2007). In Africa, various hormonal substances (Ovaprim, carp pituitary, human chorionic gonadotropin, frog pituitary extracts, etc,) have been used to induce breeding in fish with varying magnitudes of success (Nwadukwe 1993;Okoro et al, 2007). There is, therefore, the need to carry out comparative studies on the effectiveness of these induction agents in order to define the viable options.…”