2009
DOI: 10.1007/s00418-009-0647-4
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Induction and cellular expression of tartrate resistant acid phosphatase during dextran sodium sulphate induced colitis in rats

Abstract: The aim of this study was to investigate the cellular and molecular expression of tartrate resistant acid phosphatase (TRAP) as a marker of activated macrophages in macrophage dependent dextran sulphate sodium (DSS)-induced colitis in rats. In normal colon, TRAP+/CX(3)CR(1)+ macrophages were located in the upper part of the lamina propria. In the early stage (day 1-3) of acute colitis prior to histopathological changes, induction of the cytokines TNFalpha, IL-12 and IFN gamma occurred concomitant with increase… Show more

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Cited by 5 publications
(4 citation statements)
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“…Based on the data shown in the preceding paragraph it was suggestive to attribute the reduced cell growth to a reduced level of biologically active RANKL due to the observed higher expression level of OPG , in response to silicate exposure. It had been described that RANKL is required for the differentiation and proliferation of RAW 264.7 cells [Lång et al, 2009; Witte, 2010]. In order to determine the differentiation state of the RAW 264.7 cells, the established osteoclast marker protein TRAP was analyzed.…”
Section: Resultsmentioning
confidence: 99%
“…Based on the data shown in the preceding paragraph it was suggestive to attribute the reduced cell growth to a reduced level of biologically active RANKL due to the observed higher expression level of OPG , in response to silicate exposure. It had been described that RANKL is required for the differentiation and proliferation of RAW 264.7 cells [Lång et al, 2009; Witte, 2010]. In order to determine the differentiation state of the RAW 264.7 cells, the established osteoclast marker protein TRAP was analyzed.…”
Section: Resultsmentioning
confidence: 99%
“…TR-AP is a serum marker for activated macrophages and chronic inflammation and is being explored for diagnosis of chronic inflammatory diseases (54). TR-AP-positive macrophages reside in the lamina propria of the healthy colon, and a histopathological increase of TR-AP expression has been found in colitis-induced rats (55). NEC has to our knowledge not previously been linked to TR-AP.…”
Section: Discussionmentioning
confidence: 99%
“…Characterization of J8 shows that J8 exhibits a macrophage phenotype dissimilar from the parental RAW264.7, one that is unable to fully differentiate to an OC. However, macrophage phenotypes are diverse and plastic (e.g., in vivo also TRAP-positive macrophages are common [35][36][37]). Therefore, J8 does not represent macrophages in general.…”
Section: Discussionmentioning
confidence: 99%