Induction and development of nodular cluster cultures in<i>Vriesea reitzii</i>(Leme and Costa), an endangered bromeliad from the Brazilian Atlantic Forest

Vesco, Lírio Luiz Dal; Vieira, Paulo Freire; Corredor, Jenny Paola; Pescador, Rosete; Welter, Leocir José; Guerra, Miguel Pedro

doi:10.1080/14620316.2014.11513118

Cited by 11 publications

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“…On the other hand, the induction of NCs from leaf explants of V. reitzii began with the formation of protrusions at the base of the leaf. Alves et al [ 5 ], Dal Vesco et al [ 6 ] and Dal Vesco and Guerra [ 3 ] also reported the formation of NCs in the basal parts of leaf explants in V. reitzii . The formation of similar structures in this region of the explant has also been observed in other species of bromeliads, including Dickia macedoi [ 36 ], Neoregelia cruenta [ 37 ], V. friburgensis var.…”

Section: Discussionmentioning

confidence: 99%

“…NCs are globular meristematic cell clusters of a greenish-yellow color and with friable or slightly compact texture. NCs are regarded as an alternative morphogenetic pathway in plant tissue cultures [ 3 4 ], and as such, the development of nodular cultures has been described in several bromeliads, such as Vriesea reitzii [ 3 5 6 ], Bilbergia zebrina [ 4 ], and, more recently, Ananas comosus var. comosus [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…One strategy for inducing NCs in bromeliads primarily uses leaf bases [ 3 6 7 ], although induction from nodal segments [ 4 ] and seeds has also been reported [ 12 ]. Moreover, similar structures were induced from floral segments [ 13 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)

Corredor-Prado

Schmidt

Guerra

et al. 2015

J Microsc Ultrastruct

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Micropropagation via induction, multiplication and development of nodular cultures (NCs) is an efficient regeneration system for Bromeliaceae, a family of endangered monocot plants with ornamental value. Therefore, the present work aimed to induce NCs from seeds and leaf explants of Vriesea in order to characterize the morphological and histochemical aspects of induction and formation of these cultures. Seeds of Vriesea friburgensis var. paludosa were sterilized and inoculated into liquid culture media supplemented with different concentrations and combinations of growth regulators. Leaf explants of Vriesea reitzii were inoculated into medium supplemented with 4 μM α-naphthalene acetic acid (NAA) and 2 μM 6-benzylaminopurine (BAP). The addition of NAA (4 μM) in the culture medium used for seeds led to an induction rate of 72% in NCs. First, the embryo began to germinate, and afterwards, nodular structures started to form. While NCs formed from seeds is associated with root and shoot meristems, the formation of NCs from leaf explants involves the intercalary meristem. Meristematic cells generate an appropriate response in the induction medium, producing NCs by the proliferation of small cells with meristematic characteristics and large vacuolated cells. These results provide a better understanding of morphogenetic responses in bromeliads and, hence, the opportunity to develop optimized micropropagation protocols.Abbreviations: BAP, 6-benzylaminopurine; 2-iP, N6 (2-isopentyl) adenine; CBB, Coomassie Brilliant Blue; CLSM, confocal laser scanning microscopy; MSB, MS basal medium; NAA, α-Naphthalene acetic acid; NCs, nodular cultures; PAS, Periodic Acid-Schiff; SEM, scanning electron microscopy; TDZ, N-phenyl-N’-1,2,3-thidiazol-5-ylurea; TB-O, Toluidine Blue O; TEM, Transmission electron microscopy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)

Corredor-Prado

Schmidt

Guerra

et al. 2015

J Microsc Ultrastruct

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“…NCs are defined as groups or conglomerates of organogenic nodules with high regenerative efficiency Dal Vesco et al, 2011). Strategies for induction of NCs in bromeliads include nodal segments (Dal Vesco, Stefenon, Welter, Guerra, & Scherer, 2011), leaf segments (Alves, Dal Vesco, & Guerra, 2006;Scherer et al, 2013;Dal Vesco, Vieira, Corredor Prado, Guerra, & Elter, 2014b) and seeds (Dal Vesco, Pescador, Corredor Prado, Welter, & Guerra, 2014a;Corredor-Prado et al, 2015). This work has established a system of NCs induction and regeneration of shoots in response to different types and combinations of plant growth regulators (PGRs).…”

Section: Introductionmentioning

confidence: 99%

Dynamics of proteins, carbohydrates and global DNA methylation patterns during induction of nodular cluster cultures from seeds of Vriesea reitzii

et al. 2020

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Tissue culture techniques have been employed for bromeliad mass propagation by means of the morphogenetic route of nodular cluster cultures (NCs). This study aimed to assess protein, carbohydrate and global DNA methylation (GDM) level dynamics during NCs induction from Vriesea reitzii seeds. Seeds were inoculated into Murashige and Skoog (MS) liquid medium supplemented with 4 µM α-naphthaleneacetic acid (NAA) to induce NCs and in culture medium without plant growth regulators to form normal seedlings. Samples collected at 0, 3, 7, 10, 14, and 21 days of culture were analyzed. All parameters assessed showed the same variation pattern. However, seeds inducing NCs showed significantly lower starch (6.0 mg g-1 FM), carbohydrate (10.7 mg g-1 FM) and GDM (11.0%) levels than seeds forming normal seedlings after 21 days in culture. On the other hand, the protein content (9.1 mg g-1 FM) was significantly higher during induction. NCs induction process through seeds is the result of gene reprogramming in the explant, which leads to morphological, biochemical and metabolic alterations. This involves dedifferentiation, high cell proliferation, high energy demand and protein synthesis, which is related to elevated metabolic activity.

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“…y N. procerum Lindman (Lopes da Silva et al 2012), Ananas comosus (L.) Merr. (Al-saif et al 2011, Scherer et al 2013, Vriesea reitzii Leme & A. Costa (Dal-Vesco et al 2014), Dyckia distachya Hassler (Pompelli & Guerra 2005) y más específicamente en Puya, en especies como Puya chilensis Molina (1995 citado por Fuenzalida 2003, Lopéz et al 2001citado por Cabello & Suazo 2007, Puya santosii Cuatrec. (Pedroza & Bejarano 2008) y Puya berteroniana Mez.…”

Section: Introductionunclassified

Micropropagación y determinación del número cromosómico de Puya trianae con fines de conservación y uso ornamental

2018

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Puya trianae se caracteriza por desarrollar una vistosa inflorescencia que la caracteriza como planta de uso ornamental. Aunque actualmente no se considera amenazada, debido al rápido deterioro de los ecosistemas donde habita, la supervivencia de sus poblaciones sí está amenazada; por dichas razones se desarrolló un protocolo de micropropagación, aplicable para su conservación y para producción masiva de plántulas con fines ornamentales, así como a la determinación de su número cromosómico. Para la multiplicación se utilizaron plántulas provenientes de semillas germinadas in vitro realizando cuatro ensayos para determinar tipo, concentración y tiempo de exposición de algunos reguladores, además de la escisión parcial o total de explantes. Durante la fase de enraizamiento se cultivaron brotes en MS/2 solo y/o suplementado con AIB y ANA; la aclimatización de las plántulas obtenidas se realizó en diferentes mezclas de tierra:turba:capote, en cuarto de crecimiento e invernadero. Los resultados mostraron que en MS+2.0 mgL-1 de TDZ durante 45 días se produjo la mayor cantidad de yemas y brotes que terminaron su desarrollo después de 90 días. Los brotes enraizaron en MS/2 cuantificándose 70% de enraizamiento. Se registró una viabilidad del 90% y una longitud de plántulas de 3.6 cm en tierra:turba después de 60 días de aclimatización. El conteo cromosómico se realizó en células meristemáticas radicales tratadas con colchicina al 0.5%, fijadas en carnoy, hidrolizadas con HCL y con enzimas y teñidas con orceína acética, obteniéndose un número cromosómico de 50, con cromosomas muy pequeños (aprox. 1.2μm).

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Induction and development of nodular cluster cultures inVriesea reitzii(Leme and Costa), an endangered bromeliad from the Brazilian Atlantic Forest

Cited by 11 publications

References 1 publication

Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)

Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)

Dynamics of proteins, carbohydrates and global DNA methylation patterns during induction of nodular cluster cultures from seeds of Vriesea reitzii

Micropropagación y determinación del número cromosómico de Puya trianae con fines de conservación y uso ornamental

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