Induction and partial purification of bacteriophages from Desulfovibrio vulgaris (Hildenborough) and Desulfovibrio desulfuricans ATCC 13541

Seyedirashti, Seyedreza; Wood, Charles; Akagi, J. M.

doi:10.1099/00221287-137-7-1545

Cited by 13 publications

(5 citation statements)

References 25 publications

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“…The absence of common lysogenic phage and near identity to strain Hildenborough suggested that strain DePue might serve as a host for the temperate bacteriophage previously shown to be induced in strain Hildenborough (Handley et al ., 1973; Seyedirashti et al ., 1991; 1992). These phages could not be propagated on other Desulfovibrio or enteric bacteria ( Escherichia coli or Salmonella typhimurium ) as they failed to form plaques on these strains (Seyedirashti et al ., 1991). Using a standard plaque assay, we recovered infectious particles from plaques formed upon bacterial lawns of strain DePue.…”

Section: Resultsmentioning

confidence: 99%

“…Genes coding for tail‐fibre proteins, Cro/cI‐transcriptional regulators and phage‐integrase proteins suggest the presence of two other undefined bacteriophage within D. vulgaris Hildenborough. This annotation is consistent with earlier studies that demonstrated the induction of phage‐like particles using mitomycin C or UV radiation (Handley et al ., 1973; Seyedirashti et al ., 1991). Although molecular characterization demonstrated that these particles were comprised of at least two distinct viruses (Seyedirashti et al ., 1992), the lack of a suitable indicator strain prevented further studies of Desulfovibrio bacteriophage biology.…”

Section: Introductionmentioning

confidence: 99%

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Recovery of temperate Desulfovibrio vulgaris bacteriophage using a novel host strain

Walker

Stolyar

Pinel

et al. 2006

Environmental Microbiology

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A novel sulfate-reducing bacterium (strain DePue) closely related to Desulfovibrio vulgaris ssp. vulgaris strain Hildenborough was isolated from the sediment of a heavy-metal impacted lake using established techniques. Although few physiological differences between strains DePue and Hildenborough were observed, pulse-field gel electrophoresis (PFGE) revealed a significant genome reduction in strain DePue. Comparative whole-genome microarray and polymerase chain reaction analyses demonstrated that the absence of genes annotated in the Hildenborough genome as phage or phage-related contributed to the significant genome reduction in strain DePue. Two morphotypically distinct temperate bacteriophage from strain Hildenborough were recovered using strain DePue as a host for plaque isolation.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Recovery of temperate Desulfovibrio vulgaris bacteriophage using a novel host strain

Walker

Stolyar

Pinel

et al. 2006

Environmental Microbiology

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“…Nonexposed bacterial cultures served as control. The UV-dose was measured with the culture diluting method (Seyedirashcti et al 1991(Seyedirashcti et al , 1992. Two samples were examined: an LD 50 cell suspension and another sample, after 1 min of UV irradiation.…”

Section: Induction Of Prophagementioning

confidence: 99%

Significance of lysogeny for the metabolism ofDesulfovibriospp. strains isolated from aquatic environments of Georgia

Balarjishvili¹,

Kvachadze²,

Tevdoradze³

et al. 2021

J of Applied Microbiology

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Aims: Sulphate-reducing bacteria (SRB) are ecologically important group of anaerobic micro-organisms that can reduce sulphate to form hydrogen sulphide-a toxic gas causing iron corrosion on metal surfaces. In this work, SRB strains were isolated from aquatic environments in the country of Georgia to determine their lysogenicity and the role of temperate phages in host metabolism. Methods and Results: SRB strains were isolated in samples from the Black Sea coast of Georgia. Based on their genetic, cytological and physiological properties of bacteria, 10 Georgian isolates were assigned to the genus Desulfovibrio. Temperate bacteriophages were induced from three out of ten strains by UV-exposure. Comparison of metal (Fe and Cr) reduction and utilization of various carbon sources by the wild-type (lysogenic) bacterial strains and their UV-irradiated counterparts was done. Conclusions: Temperate phage in the cells of SRB could alter significant functions of bacteria and may have a contribution in the acquisition of different traits by SRB. Significance and Impact of the Study: This article pointed to a significant role for temperate bacteriophages in the metabolism and metabolic potential of host strains of SRB, which were first isolated from the aquatic environment of Georgia.

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“…At the level of pure cultures, traditional recovery approaches using mitomycin C or UV radiation has provided evidence that phages are capable to infect the model strain Desulfovibrio vulgaris str. Hildenborough [ 20 , 21 , 22 ]. Further reports on co-culture of phages extracted from D. vulgaris str.…”

Section: Introductionmentioning

confidence: 99%

Ecophysiological Features Shape the Distribution of Prophages and CRISPR in Sulfate Reducing Prokaryotes

et al. 2021

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Sulfate reducing prokaryotes (SRP) are a phylogenetically and physiologically diverse group of microorganisms that use sulfate as an electron acceptor. SRP have long been recognized as key players of the carbon and sulfur cycles, and more recently, they have been identified to play a relevant role as part of syntrophic and symbiotic relations and the human microbiome. Despite their environmental relevance, there is a poor understanding about the prevalence of prophages and CRISPR arrays and how their distribution and dynamic affect the ecological role of SRP. We addressed this question by analyzing the results of a comprehensive survey of prophages and CRISPR in a total of 91 genomes of SRP with several genotypic, phenotypic, and physiological traits, including genome size, cell volume, minimum doubling time, cell wall, and habitat, among others. Our analysis discovered 81 prophages in 51 strains, representing the 56% of the total evaluated strains. Prophages are non-uniformly distributed across the SRP phylogeny, where prophage-rich lineages belonged to Desulfovibrionaceae and Peptococcaceae. Furthermore, our study found 160 CRISPR arrays in 71 SRP, which is more abundant and widely spread than previously expected. Although there is no correlation between presence and abundance of prophages and CRISPR arrays at the strain level, our analysis showed that there is a directly proportional relation between cellular volumes and number of prophages per cell. This result suggests that there is an additional selective pressure for strains with smaller cells to get rid of foreign DNA, such as prophages, but not CRISPR, due to less availability of cellular resources. Analysis of the prophage genes encoding viral structural proteins reported that 44% of SRP prophages are classified as Myoviridae, and comparative analysis showed high level of homology, but not synteny, among prophages belonging to the Family Desulfovibrionaceae. We further recovered viral-like particles and structures that resemble outer membrane vesicles from D. vulgaris str. Hildenborough. The results of this study improved the current understanding of dynamic interactions between prophages and CRISPR with their hosts in both cultured and hitherto-uncultured SRP strains, and how their distribution affects the microbial community dynamics in several sulfidogenic natural and engineered environments.

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Induction and partial purification of bacteriophages from Desulfovibrio vulgaris (Hildenborough) and Desulfovibrio desulfuricans ATCC 13541

Cited by 13 publications

References 25 publications

Recovery of temperate Desulfovibrio vulgaris bacteriophage using a novel host strain

Recovery of temperate Desulfovibrio vulgaris bacteriophage using a novel host strain

Significance of lysogeny for the metabolism ofDesulfovibriospp. strains isolated from aquatic environments of Georgia

Ecophysiological Features Shape the Distribution of Prophages and CRISPR in Sulfate Reducing Prokaryotes

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