Induction of apoptosis against cancer cell lines by four ascomycetes (endophytes) from Malaysian rainforest

Hazalin, Nurul Aqmar Mohamad Nor; Ramasamy, Kalavathy; Lim, Siong Meng; Cole, A. L. J.; Majeed, Abu Bakar Abdul

doi:10.1016/j.phymed.2012.01.007

Cited by 14 publications

(7 citation statements)

References 43 publications

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“…With the advancement in research, endophytic fungi have emerged as a huge treasure of natural drug screening due to the ability to produce many novel, drug‐efficient compounds . At the same time, endophytic fungi may be this alternative resource based on their recently identified ability to produce the same or similar compounds as those found in their host plants .…”

Section: Introductionmentioning

confidence: 99%

Changes of volatile organic compounds and bioactivity of Alternaria brassicae GL07 in different ages

et al. 2019

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Plant endophytes are rich in secondary metabolites and are widely used in medicine, chemical, food, agriculture, and other fields. Here, an endophytic fungus is isolated from Ginkgo biloba L. leaves and identified as Alternaria brassicae GL07 through genotypic characterizations. It can produce fruity scented volatiles. The analysis of volatile organic compounds (VOCs) was done by gas chromatography–mass spectrometry. A total of 32 components were identified; and at different culture times, the composition of VOCs was different. It had more components in the first two weeks, but a fewer components on the 21st day. More olefins, ketone, aldehyde, and alcohol were found in the growth period and more amines and esters were found in the decline period. Also, 2,5‐dihydroxyacetophenone, β‐ionone, and nonanal were found. They were the same ingredients in Ginkgo essential oils and some of them were isolated for the first time in the volatile constituents of endophytes. The antioxidant activity and whitening activities of all extracts were also evaluated. When cultured for 10 days, it had the strongest 2,2‐diphenyl‐2‐picrylhydrazyl radical (IC50, 0.56 g/L), hydroxyl radical scavenging ability (IC50, 0.47 g/L), reducing ability, and tyrosinase inhibition ability (IC50, 5.18 g/L), which may be due to a large amount of ketones and alcohols produced during the log phase. This demonstrates the potential of A. brassicae GL07 to produce bioactive compounds and to be used for perfume and cosmetic industries.

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Section: Introductionmentioning

confidence: 99%

Changes of volatile organic compounds and bioactivity of Alternaria brassicae GL07 in different ages

et al. 2019

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“…The cytocompatibility of the respective formulated bead [Alg‐XG‐β‐CD(0.5)‐Ch, Alg‐XG‐β‐CD(0.8)‐Ch and Alg‐XG‐β‐CD(1.0)‐Ch] against BJ cells was assessed using the MTT assay as described previously by Hazalin et al . (). Briefly, BJ cells were seeded onto the 96 flat bottom well plates at a density of 1 × 10 4 cells per well and incubated overnight to allow attachment.…”

Section: Methodsmentioning

confidence: 97%

Microencapsulation of Lactobacillus SP. Using Chitosan‐Alginate‐Xanthan Gum‐β‐Cyclodextrin and Characterization of its Cholesterol Reducing Potential and Resistance Against pH, Temperature and Storage

Fareez

Lim

et al. 2016

J Food Process Engineering

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Probiotics are increasingly recognized for their cholesterol removal ability. Their vulnerability to harsh environmental factors during gastrointestinal transit and industrial processing remains a major challenge. This study addressed these issues by immobilizing Lactobacillus plantarum LAB12 within polymeric matrix comprised of sodium alginate (NaAlg), xanthan gum (XG) and chitosan (Ch) coating, with supplementation of b-cyclodextrin (b-CD), another potential cholesterol reducing agent. LAB12 encapsulated in Alg-XG-b-CD-Ch exhibited excellent survivability (95%) at pH 1.8 with facilitated release (>9 log CFU/g) at pH 6.8. While minimizing the loss of viable microencapsulated LAB12 at 75 and 908C, the microcapsules yielded viable counts >7 log CFU/g over a 4-week storage at 48C. Cholesterol lowering effects of b-CD supplemented beads and LAB12 against total cholesterol from egg yolk were also studied. These findings implied the potential use of Alg-XG-b-CD-Ch as protective micro-transport for probiotics and additional means of cholesterol reduction. PRACTICAL APPLICATIONSThe Alg-XG-b-CD-Ch beads yielded in this study exhibited characteristics of an ideal probiotic vehicle whereby they enhanced protection of free lactic acid bacteria (LAB) against harsh environments, facilitated targeted-cell release and conferred health benefits to the host upon consumption. The technical applications of this formulation include its use as heat-and pH-stable polymeric beads that carry L. plantarum LAB12 (the LAB model). The beads are acid tolerant (pH 1.8) under gastric conditions with the capability of targeted release at the large intestine (pH 6.8). In addition, it is also heat resistant, enabling microorganisms to survive at high temperature of up to 908C (essential during product manufacturing). The bound b-CD within the formulation is also able to enhance the cholesterol reducing ability of the LAB. Alg-XG-b-CD-Ch have successfully widen the applications of probiotics especially in health, food and agro industries.

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“…The target rDNA region, including the internal transcribed spacer (ITS1, ITS2) regions and 5.8S gene, was amplified using ITS1 (5 0 -TCCGTAGGTGAACCTGCGG-3 0 ), a specific primer for fungi, and ITS4 (5 0 -TCCTCCGC TTATTGATATGC-3 0 ), a universal primer [27]. Cultures were incubated, without shaking, at 281C for 3-5 days.…”

Section: Dna Amplification Pcr Amplification and Sequencingmentioning

confidence: 99%

Apoptosis induced by desmethyl-lasiodiplodin is associated with upregulation of apoptotic genes and downregulation of monocyte chemotactic protein-3

Hazalin

Lim²,

Cole

et al. 2013

Anti-Cancer Drugs

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There is growing interest in the discovery of bioactive metabolites from endophytes as an alternative source of therapeutics. Identification of their therapeutic targets is essential in understanding the underlying mechanisms and enhancing the resultant therapeutic effects. As such, bioactive compounds produced by endophytic fungi from plants at the National Park, Pahang, Malaysia, were investigated. Five known compounds were identified using LC-UV-MS-NMR and they include trichodermol, 7-epi-brefeldin A, (3R,4S)-4-hydroxymellein, desmethyl-lasiodiplodin and cytochalasin D. The present study went on to investigate the potential anticancer effects of these compounds and the corresponding molecular mechanisms of the lead compound against human breast adenocarcinoma, MCF-7. For the preliminary screening, the cytotoxicity and apoptotic effects of these compounds against MCF-7 were examined. The compounds were also tested against noncarcinogenic hepatocytes (WRL68). The differential cytotoxicity was then determined using the MTT assay. Desmethyl-lasiodiplodin was found to suppress the growth of MCF-7, yielding an inhibitory concentration (IC50) that was seven-fold lower than that of the normal cells. The cytotoxic effect of desmethyl-lasiodiplodin was accompanied by apoptosis. Subsequent analysis demonstrated increased expression levels of caspase 3, c-myc and p53. Further, desmethyl-lasiodiplodin resulted in inhibition of monocyte chemotactic protein (MCP)-3, a cytokine involved in cell survival and metastasis. Hence, this study proposed that desmethyl-lasiodiplodin inhibited growth and survival of MCF-7 through the induction of apoptosis. This anticancer effect is mediated, in part, by upregulation of apoptotic genes and downregulation of MCP-3. As desmethyl-lasiodiplodin elicited minimal impact against normal hepatocytes, our findings also imply its potential use as a specific apoptotic agent in breast cancer treatment.

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Induction of apoptosis against cancer cell lines by four ascomycetes (endophytes) from Malaysian rainforest

Cited by 14 publications

References 43 publications

Changes of volatile organic compounds and bioactivity of Alternaria brassicae GL07 in different ages

Changes of volatile organic compounds and bioactivity of Alternaria brassicae GL07 in different ages

Microencapsulation of Lactobacillus SP. Using Chitosan‐Alginate‐Xanthan Gum‐β‐Cyclodextrin and Characterization of its Cholesterol Reducing Potential and Resistance Against pH, Temperature and Storage

Apoptosis induced by desmethyl-lasiodiplodin is associated with upregulation of apoptotic genes and downregulation of monocyte chemotactic protein-3

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