Induction of apoptosis and apoptotic mediators in balb/C splenic lymphocytes by dietary n−3 and n−6 fatty acids

Avula, C. P. Reddy; Zaman, Asif; Lawrence, Richard; Fernandes, Gabriel

doi:10.1007/s11745-999-0441-1

Cited by 44 publications

(28 citation statements)

References 70 publications

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“…Moreover, increased apoptosis was observed also in normal splenic lymphocytes obtained from Balb/c mice treated with a fish oil diet [158]. Either a decrease of Bcl-2 and an increase of FAS-ligand was observed in the lymphocytes, confirming in this case the possible induction of both the intrinsic and extrinsic apoptotic pathways by n-3 PUFAs.…”

Section: Other Cancer Cellsmentioning

confidence: 52%

Dietary polyunsaturated fatty acids as inducers of apoptosis: implications for cancer

et al. 2009

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It has recently become clear the role played by alterations in apoptosis during the development of several chronic diseases (i.e. inflammatory, neurodegenerative and neoplastic pathologies). For this reason, the research for possible therapeutic strategies involving the modulation of the apoptotic pathways has attracted considerable interest in the past few years. In particular, it has been shown that apoptosis may be induced or inhibited by a variety of nutritional compounds providing health benefits. The aim of this review is to examine the ability of different dietary polyunsaturated fatty acids (PUFAs) to induce apoptosis, especially in the cancer field. The molecular effects of different PUFAs found in dairy products, meat, fish, vegetable seeds and oils, and known to affect the incidence and progression of cancer and other chronic diseases, will be analyzed. To this aim, our effort will concentrate in critically reviewing the published works concerning the effects of: (a) the n-6 PUFAs gamma-linolenic acid, arachidonic acid, and conjugated linoleic acid; (b) the n-3 PUFAs eicosapentaenoic acid and docosahexaenoic acid on the apoptotic process. We will also pay attention to the recent findings regarding the possible role of PUFAs as regulators of the endoplasmic reticulum stress-pathway of apoptosis.

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Section: Other Cancer Cellsmentioning

confidence: 52%

Dietary polyunsaturated fatty acids as inducers of apoptosis: implications for cancer

et al. 2009

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“…In particular, it was shown that DHA was able to alter the expression and the activity of caspase-8 and -9 in colon cancer and HL-60 lymphoma cells [59,60]. Moreover, EPA induced an increased activity of caspase-3 and -9 in the lymphoma Ramos cell line [59] and dietary n-3 PUFAs were able to decrease BCL-2 and increase FAS-ligand expression in normal splenic lymphocytes of Balb/c mice [61]. Alteration of BCL-2 family proteins, cytochrome c release and mitochondrial membrane depolarization were induced by EPA and DHA in colon cancer cells [3,62,63].…”

Section: Discussionmentioning

confidence: 99%

Docosahexaenoic acid induces apoptosis in lung cancer cells by increasing MKP-1 and down-regulating p-ERK1/2 and p-p38 expression

et al. 2008

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Different agents able to modulate apoptosis have been shown to modify the expression of the MAP-kinase-phosphatase-1 (MKP-1). The expression of this phosphatase has been considered a potential positive prognostic factor in lung cancer, and smoke was shown to reduce the levels of MKP-1 in ferret lung. Our aim was to assess whether the n-3 polyunsaturated fatty acid docosahexaenoic acid (DHA), known to inhibit the growth of several cancer cells mainly inducing apoptosis, may exert pro-apoptotic effect in lung cancer cells by modifying MKP-1 expression. We observed that DHA increased MKP-1 protein and mRNA expression and induced apoptosis in different lung cancer cell lines (mink Mv1Lu adenocarcinoma cells, human A549 adenocarcinoma and human BEN squamous carcinoma cells). We inhibited the pro-apoptotic effect of DHA by treating the cells with the phosphatase inhibitor Na(3)VO(4) or by silencing the MKP-1 gene with the specific siRNA. This finding demonstrated that the induction of apoptosis by DHA involved a phosphatase activity, specifically that of MKP-1. DHA reduced also the levels of the phosphorylated MAP-kinases, especially ERK1/2 and p38. Such an effect was not observed when the MKP-1 gene was silenced. Altogether, the data provide evidence that the DHA-induced overexpression of MKP-1 and the resulting decrease of MAP-kinase phosphorylation by DHA may underlie the pro-apoptotic effect of this fatty acid in lung cancer cells. Moreover, they support the hypothesis that DHA may exert chemopreventive action in lung cancer.

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“…Animal studies have clearly demonstrated that the content of long-chain PUFA n-3 fatty acids and/or the ratio of n-6/n-3 fatty acids in the diet modulates many measures of Tcell function. These include the ability to proliferate in response to mitogen/antigen stimulation and to mount a delayed-type hypersensitivity response (68)(69)(70)(71)(72)(73). The physiological interpretation of how T-cell function is altered by these lipids is far from clear.…”

Section: Pufa and T-cell Function (Adult And Animals)mentioning

confidence: 99%

Polyunsaturated fatty acids and T‐cell function: Implications for the neonate

Field

Clandinin

Aerde

2001

Lipids

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Infant survival depends on the ability to respond effectively and appropriately to environmental challenges. Infants are born with a degree of immunological immaturity that renders them susceptible to infection and abnormal dietary responses (allergies). T-lymphocyte function is poorly developed at birth. The reduced ability of infants to respond to mitogens may be the result of the low number of CD45RO+ (memory/antigen-primed) T cells in the infant or the limited ability to produce cytokines [particularly interferon-y, interleukin (IL)-4, and IL-10. There have been many important changes in optimizing breast milk substitutes for infants; however, few have been directed at replacing factors in breast milk that convey immune benefits. Recent research has been directed at the neurological, retinal, and membrane benefits of adding 20:4n-6 (arachidonic acid; AA) and 22:6n-3 (docosahexaenoic acid; DHA) to infant formula. In adults and animals, feeding DHA affects T-cell function. However, the effect of these lipids on the development and function of the infant's immune system is not known. We recently reported the effect of adding DHA + AA to a standard infant formula on several functional indices of immune development. Compared with standard formula, feeding a formula containing DHA + AA increased the proportion of antigen mature (CD45RO+) CD4+ cells, improved IL-10 production, and reduced IL-2 production to levels not different from those of human milk-fed infants. This review will briefly describe T-cell development and the potential immune effect of feeding long-chain polyunsaturated fatty acids to the neonate.

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Induction of apoptosis and apoptotic mediators in balb/C splenic lymphocytes by dietary n−3 and n−6 fatty acids

Cited by 44 publications

References 70 publications

Dietary polyunsaturated fatty acids as inducers of apoptosis: implications for cancer

Dietary polyunsaturated fatty acids as inducers of apoptosis: implications for cancer

Docosahexaenoic acid induces apoptosis in lung cancer cells by increasing MKP-1 and down-regulating p-ERK1/2 and p-p38 expression

Polyunsaturated fatty acids and T‐cell function: Implications for the neonate

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