Induction of apoptosis by ethanol extract of &lt;i&gt;Evodia rutaecarpa&lt;/i&gt; in HeLa human cervical cancer cells &lt;i&gt;via&lt;/i&gt; activation of AMP-activated protein kinase

Park, Seon-Young; Park, Cheol; Park, Shin‐Hyung; Kim, Gi‐Young; Hong, Su‐Hyun; Choi, Yung Hyun

doi:10.5582/bst.2016.01170

Cited by 21 publications

(9 citation statements)

References 51 publications

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“…AMPK, which also known as AMP-activated protein Kinase, has been generally considered as a tumor suppressor, and its important features have been summarized by a recent study. 29 AMPK has been found to have the ability to deactivate mTOR, which further supports our observations for the adverse prognosis related miRNAs. In addition, many activators of AMPK have been found to be associated with tumor suppressing function.…”

Section: Discussionsupporting

confidence: 88%

“…In the enrichment analyses, we observed that predicted target genes of hs‐miR‐600 are related to the enriched AMPK pathway. AMPK, which also known as AMP‐activated protein Kinase, has been generally considered as a tumor suppressor, and its important features have been summarized by a recent study . AMPK has been found to have the ability to deactivate mTOR, which further supports our observations for the adverse prognosis related miRNAs.…”

Section: Discussionmentioning

confidence: 99%

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Integrative miRNA analysis identifies hsa‐miR‐3154, hsa‐miR‐7‐3, and hsa‐miR‐600 as potential prognostic biomarker for cervical cancer

Zeng

Wang²,

et al. 2017

J of Cellular Biochemistry

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MicroRNAs, which can interfere with the translation of target mRNAs, caught a particular interest as their functions are related to human cancers. As the most common cancer in women, cervical cancer remains one of the leading cancer-related causes of death. In this study, we performed an integrative miRNA analysis to identify prognosis related miRNAs for cervical cancer. In addition, we developed an n-miRNA expression signature (risk score) to comprehensively assess the prognosis of cervical cancer, especially for survival time. Furthermore, we performed target predictions and functional enrichment analyses for the identified miRNAs to investigate their potential role in the development of disease. Univariate Cox regression models were used to assess the association between miRNAs and prognosis of cervical cancer. Three miRNAs were identified to be significantly associated with survival time of patients. Hsa-miR-3154 and hsa-miR-7-3 were significantly associated with shortened survival time and more death cases, whereas expression level of hsa-miR-600 was significantly associated with prolonged survival time. The function enrichment analysis showed the target genes of poor prognosis related miRNAs were mainly enriched in the mTOR pathway, whereas target genes of positive prognosis related miRNAs were mainly enriched in the AMPK pathway. In summary, a 3-miRNA expression signature was identified which can predict cervical cancer patient survival. The potential functions of this 3-miRNA expression signature and individual miRNAs as prognostic targets of cervical cancer were revealed by this study. Furthermore, these findings may have important implications in the understanding of the potential therapeutic method for the cervical cancer patients.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Integrative miRNA analysis identifies hsa‐miR‐3154, hsa‐miR‐7‐3, and hsa‐miR‐600 as potential prognostic biomarker for cervical cancer

Zeng

Wang²,

et al. 2017

J of Cellular Biochemistry

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“…Natural products and their derivatives are gaining interest as novel therapeutic agents to treat several types of cancer because these substances can exert multiple anticancer effects and are associated with few side effects, unlike existing anticancer drugs. Among medicinal plants, ER has been reported in various studies to show anticancer effects [11,12]. Several studies on ER derivatives have already shown that evodiamine has an anticancer effect in ovarian cancer cells [34,35].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have reported that ER and its derivatives exhibit multiple biological activities, including anti-inflammatory, anti-obesity, antihypertensive, and anti-allergic effects [8][9][10]. Recently, two studies have reported that the activation of caspases and AMPactivated protein kinase by an ethanol extract of ER led to apoptosis of cervical cancer cells and benign prostatic hyperplasia epithelial cells, respectively [11,12]. The finding that the ER extract inhibits proliferation in various cell lines indicates that the plant or its components may have anticancer activity.…”

Section: Introductionmentioning

confidence: 99%

Role of limonin in anticancer effects of Evodia rutaecarpa on ovarian cancer cells

Bae

Park

Suh

et al. 2020

BMC Complement Med Ther

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Background: Ovarian cancer therapy generally involves systemic chemotherapy with anticancer drugs; however, chemotherapy with a platinum-based drug has often been shown to cause adverse reactions and drug resistance in ovarian cancer patients. Evodia rutaecarpa (ER) reportedly shows anticancer activity against various types of cancer cells. However, the effects of ER have not yet been fully uncovered in ovarian cancer. Methods: In the present study, we investigated the anticancer effects of an ER extract and its components against the ovarian cancer cell lines SKOV-33, A2780, RMUG-S and a cisplatin-resistant SKOV-3 cell line (Cis R SKOV-3). Cell viability and colony formation assays along with subcellular fractionation analysis, immunoblotting, and immunofluorescence staining were performed. Results: ER treatment led to a significant reduction in the viability of SKOV-3 cells. Moreover, limonin, a compound found in ER, reduced the viability of both serous-type (SKOV-3 and A2780) and mucinous-type (RMUG-S) ovarian cancer cells by inducing apoptosis via activation of the p53 signaling pathway. Furthermore, limonin reversed the drug resistance through activation of apoptosis in Cis R SKOV-3. Conclusion: Taken together, our findings suggest that limonin contributes to the anti-ovarian cancer effects of ER by inducing apoptosis via activation of the p53 signaling pathway.

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“…An E. rutaecarpa extract was also effective in alleviating alcohol-induced hangover symptoms in a mouse model [ 6 ]. Furthermore, an ethanol extract of E. rutaecarpa had antitumor activity in cervical cancer cells [ 7 ]. Based on the multiple therapeutic activities of E. rutaecarpa , including anti-inflammatory, antioxidative, and antiproliferative effects, a potential therapeutic action of E. rutaecarpa against a variety of diseases is expected.…”

Section: Introductionmentioning

confidence: 99%

Ethanol Extract of Evodia rutaecarpa Attenuates Cell Growth through Caspase-Dependent Apoptosis in Benign Prostatic Hyperplasia-1 Cells

et al. 2018

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The dried fruits of Evodia rutaecarpa Bentham have been used widely as a herbal medicine for the treatment of inflammatory disorders and abdominal pain. Benign prostatic hyperplasia (BPH) is a nonmalignant disease characterized by overgrowth of prostates. Despite the pharmacological efficacy of the fruits of E. rutaecarpa against various diseases, their effects against BPH have not been reported. Here, we investigated the inhibitory activity of a 70% ethanol extract of E. rutaecarpa (EEER) against BPH, and its underlying mechanisms regarding cell growth of BPH using BPH-1 cells. An in vitro 5α-reductase activity assay showed that EEER exhibited inhibitory activity against 5α-reductase. In BPH-1 cells, EEER treatment inhibited cell viability and reduced the expression of the proliferating cell nuclear antigen proliferating cell nuclear antigen (PCNA), cyclin D1, and phosphor-ERK1/2 proteins. Moreover, EEER also induced apoptosis, with chromatin condensation, apoptotic bodies, and internucleosomal DNA fragmentation. Regarding its underlying mechanisms, EEER exacerbated the activation of caspase-8 and caspase-3 in a concentration-dependent manner and eventually caused the cleavage of PARP. Taken together, these data demonstrated that EEER had a potent 5α-reductase inhibitory activity and that EEER treatment in BPH-1 cells inhibited cell viability via caspase-8- and caspase-3-dependent apoptosis. Therefore, EEER may be a potential phytotherapeutic agent for the treatment of BPH.

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Induction of apoptosis by ethanol extract of <i>Evodia rutaecarpa</i> in HeLa human cervical cancer cells <i>via</i> activation of AMP-activated protein kinase

Cited by 21 publications

References 51 publications

Integrative miRNA analysis identifies hsa‐miR‐3154, hsa‐miR‐7‐3, and hsa‐miR‐600 as potential prognostic biomarker for cervical cancer

Integrative miRNA analysis identifies hsa‐miR‐3154, hsa‐miR‐7‐3, and hsa‐miR‐600 as potential prognostic biomarker for cervical cancer

Role of limonin in anticancer effects of Evodia rutaecarpa on ovarian cancer cells

Ethanol Extract of Evodia rutaecarpa Attenuates Cell Growth through Caspase-Dependent Apoptosis in Benign Prostatic Hyperplasia-1 Cells

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