Induction of Apoptosis by Methyl Alcohol Extract of &lt;i&gt;Enteromorpha linza&lt;/i&gt; (Linnaeus) J Agardh in U937 Human Leukemia Cells

Choi, E-O; Hs, Kim; Han, M.J.; Park, C; Bw, Kim; Hwang, Ja Young; Choi, Yung Hyun; Hwang, H-J

doi:10.4314/tjpr.v13i6.8

Cited by 3 publications

(5 citation statements)

References 26 publications

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“…A marked decrease in cell viability was observed under DNX, paralleling the results acquired from the MTT analysis. Interestingly, such effects can potentially be ascribed to DNX's pro-apoptotic attributes, as delineated in the previous literature [40,41,45,65]. Despite this, in a control setting, HFF-1 cell viability remained high at 91% with a 20 µM DNX concentration.…”

Section: Discussionmentioning

confidence: 70%

Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

Kuzucu

2023

CIMB

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This research delved into the protective capacities of deinoxanthin, a carotenoid present in Deinococcus radiodurans, against UVA- and UVB-mediated skin damage using human fibroblast foreskin cells (HFF-1). Using the MTT assay, HFF-1 cells treated with 10 µM DNX displayed 20% and 31.7% higher viability than the positive (Vitamin C-treated) and negative (DNX-untreated) control groups, respectively, upon 100 mJ/cm2 UVB exposure. At 24 J/cm2 UVA, 20 µM DNX-treated cells showed 80.6% viability, exceeding the positive and negative control groups by 28.6% and 33.6%, respectively. Flow cytometry analysis revealed that cells treated with DNX and exposed to 24 J/cm2 UVA exhibited a 69.32% reduction in apoptotic processes compared to untreated cells. Similarly, when exposed to 100 mJ/cm2 UVB, DNX-treated cells demonstrated a 72.35% decrease in apoptotic processes relative to their untreated counterparts. DNX also displayed dose-dependent inhibition on tyrosinase activity. The study emphasized DNX’s antioxidative capacity, evident in its modulation of superoxide dismutase activity and measurements of Malondialdehyde and intracellular reactive oxygen species levels. DNX-treated cells exhibited higher hydroxyproline levels, suggesting healthier collagen production. Additionally, the wound-healing assay method confirmed an accelerated healing rate in DNX-treated cells. Conclusively, DNX offers significant protection against UV-induced skin damage, emphasizing its potential for skincare and therapeutics.

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Section: Discussionmentioning

confidence: 70%

Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

Kuzucu

2023

CIMB

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“…Thus, the compounds that have a potential to restore apoptosis have a great potential to develop as anticancer drugs (Sanjeewa et al 2017). Choi et al (2014) attempted to evaluate the anticancer properties of methyl alcohol extract separated from E. linza on human leukemia cells (U937). In this study, the authors demonstrated the extract has a potential to induce apoptosis in U937 cell via upregulated expression of death receptors 4 and 5 and downregulated expression of antiapoptotic proteins such as Bcl-2 and Bcl-xL and IAP family proteins (50~300 μg/mL).…”

Section: Anticancer Propertiesmentioning

confidence: 99%

Nutrients and bioactive potentials of edible green and red seaweed in Korea

2018

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Background: Traditionally, East-Asians (Korea, Japan, and China) utilize seaweeds as a food source and ingredient in traditional medicine. Korea is one of the biggest seaweed producer and consumer in the global trade. Especially, side dishes made from seaweeds are very popular in the traditional Korean cuisines. Seaweeds are popular as fresh vegetable salads and soup or eaten as snacks. Main body: Seaweeds are rich in essential nutrients, minerals, and vitamins as well as a promising source of novel bioactive compounds. The compounds (polysaccharides, polyphenols, and sterols) present in the edible Korean seaweeds possess important bioactive properties such as antioxidant, anti-inflammation, anticancer, anti-diabetic, and anticoagulant properties. Thus, the long-term consumption of seaweed has a potential to reduce the risk of cancer, diabetes, obesity, and inflammation-related complications. However, seaweed consumption is limited to the small population around the globe. Thus, it is important to increase the awareness of the health benefits of seaweeds consumption among the general population. Short conclusion: In the present study, we discussed some popular green and red edible Korean seaweeds and their health-promoting properties. This study might be useful to increase the public awareness of the consumption of seaweed as a food source.

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“…There are more than 600 different carotenoids in nature, and they are common natural pigments [19]. Although some natural carotenoids, such as astraxanthin, β-carotene, and lycopene have been used as food coloring for many years, it has recently been an area of intense study in investigating its potential to prevent epithelial cancer and chronic diseases, with the discovery of antioxidant activity [20,21].…”

Section: Introductionmentioning

confidence: 99%

“…Although some natural carotenoids, such as astraxanthin, β-carotene, and lycopene have been used as food coloring for many years, it has recently been an area of intense study in investigating its potential to prevent epithelial cancer and chronic diseases, with the discovery of antioxidant activity [20,21]. Carotenoids are found in many bacteria and are found in a wide variety of phyla, not only photosynthetic but also non-photosynthetic species [19].…”

Section: Introductionmentioning

confidence: 99%

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Deinoksantinin HER2 Pozitif Meme Kanserinde Tamoksifenin Antiproliferatif Aktivitesi Üzerindeki Agonistik Etkileri: MDA-MB-453 Üzerine Bir In vitro Çalışma

GÜNAY>

Kuzucu

2023

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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A good understanding of the pathogenesis of breast cancer, in which in 2020 an estimated 684,996 women across the world died, plays a vital role in the development of treatment methods. In recent years, estrogen receptor (ER), progesterone receptor (PR), and Epidermal Growth Factor receptor-2 (HER2) expression levels have been substantial prognostic markers. The combinational therapy approach is the use of two or more active ingredients or methods. The most important benefit of this treatment method is to reduce the development of drug resistance since the probability of the carcinoma being resistant to more than one drug at the same time is low. Tamoxifen, an estrogen receptor competitive and nonsteroidal drug, has been used for nearly 20 years to treat patients with hormone receptor-positive breast cancer. Deinoxanthin is a xanthophyll derivative purified from the cell wall of a radiation-resistant bacterium, Deinococcus radiodurans. This xanthine derivative has been shown in some studies to have proapoptotic and antiproliferative effects on some types of cancer. Within the study's scope, it aimed to increase the effectiveness of Tamoxifen with deinoxanthin. Using the ACTB gene as a reference to investigate the synergistic effect of Tamoxifen and deinoxanthin on the MDA-MB-453 breast cancer cell line; Expression levels of BAX, CASP-3, BCL-2, and HER2 genes were examined by RT-qPCR method. The ELISA method determined the amounts of BAX, CASP-3, BCL-2, and HER2 proteins and compared them with RT-qPCR results. It was determined that the antiproliferative effect of Tamoxifen on the HER2 positive breast cancer cell line was increased with the combination of deinoxanthin.

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Induction of Apoptosis by Methyl Alcohol Extract of <i>Enteromorpha linza</i> (Linnaeus) J Agardh in U937 Human Leukemia Cells

Abstract: Purpose: To investigate the anti-cancer effect of methyl alcohol extract of Enteromorpha linza (Linnaeus) J. Agardh (MEEL) in U937 human leukemia cells. Methods: Cytotoxicity was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium

Cited by 3 publications

References 26 publications

Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

Nutrients and bioactive potentials of edible green and red seaweed in Korea

Deinoksantinin HER2 Pozitif Meme Kanserinde Tamoksifenin Antiproliferatif Aktivitesi Üzerindeki Agonistik Etkileri: MDA-MB-453 Üzerine Bir In vitro Çalışma

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