Induction of B Cell Responses upon Experimental Infection of Neonatal Calves with Mycobacterium avium subsp. paratuberculosis

Stabel, Judith R.; Bannantine, John P.; Eda, Shigetoshi; Robbe‐Austerman, Suelee

doi:10.1128/cvi.00058-11

Cited by 20 publications

(17 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As reviewed by Chase et al (2008), IgM + B cells may comprise less than 5% of circulating lymphocytes, but early antigen encounters stimulate clonal expansion of this subset within weeks. Young calves that were experimentally infected intratonsillarly with MAP from 2 to 5 wk of age exhibited humoral reactivity to a ~50-kDa antigen of MAP (Waters et al, 2003), and calves exhibited B-cell responses to the pathogen by 3 mo of age (Stabel et al, 2011), demonstrating capabilities for early response. It is known that CD4 + and CD8 + T cells comprise about 20 and 10% of peripheral neonatal lymphocytes, respectively, and maintain a 2:1 ratio of relative abundance through adulthood (Chase et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…The PBMC and mesenteric node lymphocytes (MNL) were cultured in replicate 48-well flat-bottomed plates (Nunc Technologies, Rochester, NY) at 39°C in 5% CO 2 in a humidified atmosphere. For each mononuclear cell sample, 1 plate was cultured for 24 h, 1 plate was cultured for 3 d, and 1 plate was cultured for 6 d. Duplicate wells were set up for each animal for each of the following in vitro treatments: medium alone [nonstimulated (NS)], concanavalin A (10 μg/ mL; Sigma, St. Louis, MO), pokeweed mitogen (10 μg/ mL; Sigma), and phytohemagglutinin (PHA; 10 μg/ mL; Sigma), or a whole-cell sonicate preparation of MAP (MPS; 10 μg/mL; Stabel et al, 2011). After 24 h, 1 set of plates was removed and centrifuged at 400 × g for 5 min.…”

Section: Isolation and Culture Of Peripheral Blood Mononuclear Cells mentioning

confidence: 99%

See 1 more Smart Citation

Gamma delta T cells are early responders to Mycobacterium avium ssp. paratuberculosis in colostrum-replete Holstein calves

Krueger

Beitz

Humphrey

et al. 2016

Journal of Dairy Science

View full text Add to dashboard Cite

Peripheral blood mononuclear cells (PBMC) and mesenteric node lymphocytes (MNL) were obtained from 30 calves that were assigned randomly at birth to 1 of 6 treatment groups with 5 calves per treatment in a 14-d study: (1) colostrum-deprived (CD), no vitamins; (2) colostrum-replacer (CR), no vitamins; (3) CR, vitamin A; (4) CR, vitamin D; (5) CR, vitamin E; (6) CR, vitamins A, D, E. Calves were injected with appropriate vitamin supplements and fed pasteurized whole milk (CD calves) or fractionated colostrum replacer (CR calves) at birth. Thereafter, all calves were fed pasteurized whole milk fortified with vitamins according to treatment group. Calves were orally inoculated with 10 cfu of Mycobacterium avium ssp. paratuberculosis (MAP) on d 1 and 3. The PBMC and MNL harvested on d 13 were analyzed by flow cytometry as fresh cells, after 3-d culture with phytohemagglutinin (PHA), and after 6-d culture with a whole-cell sonicate of MAP (MPS). Peripheral γδ T cells were a predominant lymphocyte subset in neonatal calves, with a decreased percentage noted in CD calves compared with CR calves. As well, CD25 expression was higher in γδ T cells compared with other cell subsets, regardless of treatment group. Stimulation of PBMC with PHA resulted in increased CD4 and CD8 subsets, whereas MNL response was dominated by expansion of B-cell subpopulations. Stimulation with PHA and MPS decreased the relative abundance of PBMC γδ T cells, but MNL γδ T cells increased upon stimulation with MPS. These results identify γδ T cells as key early responders to intracellular infection in neonatal calves and suggest that colostrum may be an important mediator of this response.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Isolation and Culture Of Peripheral Blood Mononuclear Cells mentioning

confidence: 99%

Gamma delta T cells are early responders to Mycobacterium avium ssp. paratuberculosis in colostrum-replete Holstein calves

Krueger

Beitz

Humphrey

et al. 2016

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…Previously, we observed that CD25 and CD26 expression was upregulated on T and B cells within 1 month after experimental infection of calves with M. avium subsp. paratuberculosis (27,28). Expression levels of both of these activation markers were also increased on CD4, CD8, and ␥␦ T cells after stimulation of PBMCs from M. bovis-infected cattle with either a recombinant early secretory antigenic target 6 and culture filtrate protein 10 fusion protein (rESAT-6:CFP-10) or M. bovis PPD (29).…”

Section: Discussionmentioning

confidence: 85%

“…CD26 also serves as a maturation marker for T cells, and its expression is highly correlated with Th1-mediated immunity and inflammation (29,30). Further, CD26 is linked with CD45 on the surface of T cells, resulting in the induction of T-cell signal transduction pathways (28,31). The expression of CD26 appears to be preferentially upregulated on CD4 ϩ CD45RO ϩ memory T cells (32).…”

Section: Discussionmentioning

confidence: 99%

Disparate Host Immunity to Mycobacterium avium subsp.paratuberculosisAntigens in Calves Inoculated with M. avium subsp.paratuberculosis, M. avium subsp.avium, M. kansasii, and M. bovis

Stabel

Waters

Bannantine

et al. 2013

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

The cross-reactivity of mycobacterial antigens in immune-based diagnostic assays has been a major concern and a criticism of the current tests that are used for the detection of paratuberculosis. In the present study, Mycobacterium avium subsp. paratuberculosis recombinant proteins were evaluated for antigenic specificity compared to a whole-cell sonicate preparation ( A major struggle in the field diagnosis of paratuberculosis has been the cross-reactivity of serologic diagnostic tests with other mycobacteria. Of particular concern is the potential for false-positive identification of Mycobacterium avium subsp. paratuberculosis infection in animals that are either infected or have been exposed to M. avium subsp. avium (M. avium) or other environmental mycobacteria when using serum-based paratuberculosis diagnostic tests (1). Genomic studies have demonstrated a 98 to 99% homology between M. avium subsp. paratuberculosis and M. avium, making it difficult to identify antigens that will distinguish between exposures or infections with the 2 subspecies in a specific and sensitive manner (2, 3). The presence of M. avium in a wide breadth of environmental sources, such as water, soil, biofilms, and plants, as well as the contamination of feedstuffs and bedding by birds, makes cross-reactivity with current diagnostics for M. avium subsp. paratuberculosis a staunch reality (4, 5).In recent years, the reemergence of M. bovis infection in U.S. dairy herds has contributed to concerns about the cross-reactivity of M. avium subsp. paratuberculosis diagnostics in animals that are infected with or exposed to M. bovis. The development of new serodiagnostic tests based upon M. bovis antigens has improved the ability to distinguish between animals that have been either vaccinated or infected with M. avium subsp. paratuberculosis from those with bovine tuberculosis (6,7,8,9,10,11). Mycobacterium kansasii infections occur rarely in cattle, and cross-reactivity might be more of a concern with a bovine tuberculosis diagnosis; however, reports suggest that M. kansasii might share some epitopes with M. avium subsp. paratuberculosis, thereby confounding the diagnostic tests for paratuberculosis (12,13,14,15,16).Although numerous studies have evaluated M. avium subsp. paratuberculosis recombinant proteins as potential diagnostic tools, the ability of M. avium subsp. paratuberculosis proteins to discriminate between different mycobacterial infections has not been adequately addressed (17,18). This dearth of information is particularly evident for measures of cell-mediated immunity in animals exposed to different mycobacteria, as most studies report only serologic data based upon antigenspecific antibody responses. The present study is the first of its kind to compare immune responses to M. avium subsp. paratuberculosis antigens in calves infected with either live M. avium subsp. paratuberculosis, M. avium, M. bovis, or M. kansasii. Recombinant M. avium subsp. paratuberculosis proteins were compared to a whole-cell sonicate preparation of ...

show abstract

“…Although little is known about B cell contributions to immunity during M. avium subsp. paratuberculosis infections, it has been shown that B cells are highly activated in the early stages of infection, expressing CD5, a marker of antigen recognition (10,11). B cells also may become activated upon linkage of CD40 on the cell surface with CD40L present on activated T cells.…”

mentioning

confidence: 99%

Clinical Disease Upregulates Expression of CD40 and CD40 Ligand on Peripheral Blood Mononuclear Cells from Cattle Naturally Infected with Mycobacterium avium subsp. paratuberculosis

Khalifeh

Stabel

2013

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

Induction of B Cell Responses upon Experimental Infection of Neonatal Calves with Mycobacterium avium subsp. paratuberculosis

Abstract: The objective of this study was to determine if experimental infection of neonatal calves with

Cited by 20 publications

References 26 publications

Gamma delta T cells are early responders to Mycobacterium avium ssp. paratuberculosis in colostrum-replete Holstein calves

Gamma delta T cells are early responders to Mycobacterium avium ssp. paratuberculosis in colostrum-replete Holstein calves

Disparate Host Immunity to Mycobacterium avium subsp.paratuberculosisAntigens in Calves Inoculated with M. avium subsp.paratuberculosis, M. avium subsp.avium, M. kansasii, and M. bovis

Clinical Disease Upregulates Expression of CD40 and CD40 Ligand on Peripheral Blood Mononuclear Cells from Cattle Naturally Infected with Mycobacterium avium subsp. paratuberculosis

Contact Info

Product

Resources

About