Induction of c‐kit Molecules on Human CD34
 +
 /c‐kit
 &lt;low
 Cells: Evidence for CD34
 +
 /c‐kit
 &lt;low
 Cells as Primitive Hematopoietic Stem Cells

Sogo, Shinji; Inaba, Muneo; Ogata, Hiroaki; Hisha, Hiroko; Adachi, Yasushi; Mori, Shinichiro; Toki, Junko; Yamanishi, Kazuya; Kanzaki, Hideharu; Adachi, Masakazu; Ikehara, Susumu

doi:10.1002/stem.150420

Cited by 29 publications

(20 citation statements)

References 46 publications

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“…Fresh CD34-positive cells with either a Thy-1 pos , c-kit low/neg , AC133 pos or CD38 neg phenotype are enriched for more primitive progenitor cells. [44][45][46][47][48][49][50] Adhesion molecules like L-selectin, VLA-4 and VLA-5, and the chemokine receptor CXCR4 are likely associated with engraftment. [51][52][53] No significant changes occurred in the expression of early markers after 7 days under all conditions.…”

Section: Discussionmentioning

confidence: 99%

Storage of unprocessed G-CSF-mobilized whole blood in a modified Leibovitz's L15 medium preserves clonogenic capacity for at least 7 days

Kreuk

Jonkhoff

Zevenbergen

et al. 2001

Bone Marrow Transplant

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Summary:Autologous stem cell transplantation using unprocessed, G-CSF-mobilized whole blood (WB) is a simple, cost-reducing procedure and supports high-dose chemotherapy regimens not exceeding 72 h. Thereafter, clonogenic capacity rapidly decreases if routine anticoagulants are used for storage. In order to increase clinical applicability, we investigated the requirements for optimal preservation of unprocessed WB for 7 days. During storage at 22؇C in CPDA-1, a decrease in pH was noted, which was at least partially responsible for the low recovery of clonogenic cells. Subsequently, WB cells were stored in various cell culture media (RPMI 1640, ␣-MEM, X-VIVO15, CellGro SCGM and Leibovitz's L15 medium) containing either serum, serum-free substitutes or no additives. Leibovitz's L15 showed significantly better CFU-GM recoveries than the other media. Using a calcium-free modification of L15 medium (added 3:10 to WB), 94 ؎ 24% of CD34 ؉ cells, 41 ؎ 14% of BFU-E, 56 ؎ 17% CFU-GM and 90 ؎ 14% of LTC-IC were preserved during storage for 7 days at 22؇C. Storage at 4؇C was also feasible, but showed less optimal recoveries of 52 ؎ 29% (CD34), 32 ؎ 10% (BFU-E), 13 ؎ 7% (CFU-GM) and 58 ؎ 9% (LTC-IC). The expression of CD38, Thy-1, c-kit, AC133, L-selectin and CXCR4 on CD34-positive cells remained unchanged. In conclusion, a modified Leibovitz's L15 medium better meets the metabolic requirements of a high-density cell culture and allows safe storage of G-CSF mobilized WB for at least 7 days. The results encourage further exploration of WB transplants stored for 7 days for clinical use. Bone Marrow Transplantation (2001) 28, 145-155.

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Section: Discussionmentioning

confidence: 99%

Storage of unprocessed G-CSF-mobilized whole blood in a modified Leibovitz's L15 medium preserves clonogenic capacity for at least 7 days

Kreuk

Jonkhoff

Zevenbergen

et al. 2001

Bone Marrow Transplant

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“…The cell surface antigens analyzed include HLA-DR, the receptor tyrosine kinases c-kitR (CD117) and Tie2 (CD202b), the interleukin-3 cytokine receptor (IL-3R, CD123) and CD33. Although all of these antigens have been well documented to be expressed by various classes of human hematopoietic PCs, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57 the number of studies investigating their expression by human HSCs is sparse. 58, 59, 60, 61, 62 …”

Section: Introductionmentioning

confidence: 99%

Further phenotypic characterization of the primitive lineage− CD34+CD38−CD90+CD45RA− hematopoietic stem cell/progenitor cell sub-population isolated from cord blood, mobilized peripheral blood and patients with chronic myelogenous leukemia

Wisniewski

Affer

Willshire

et al. 2011

Blood Cancer Journal

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The most primitive hematopoietic stem cell (HSC)/progenitor cell (PC) population reported to date is characterized as being Lin−CD34+CD38−CD90+CD45R. We have a long-standing interest in comparing the characteristics of hematopoietic progenitor cell populations enriched from normal subjects and patients with chronic myelogenous leukemia (CML). In order to investigate further purification of HSCs and for potential targetable differences between the very primitive normal and CML stem/PCs, we have phenotypically compared the normal and CML Lin−CD34+CD38−CD90+CD45RA− HSC/PC populations. The additional antigens analyzed were HLA-DR, the receptor tyrosine kinases c-kit and Tie2, the interleukin-3 cytokine receptor, CD33 and the activation antigen CD69, the latter of which was recently reported to be selectively elevated in cell lines expressing the Bcr-Abl tyrosine kinase. Notably, we found a strikingly low percentage of cells from the HSC/PC sub-population isolated from CML patients that were found to express the c-kit receptor (<1%) compared with the percentages of HSC/PCs expressing the c-kitR isolated from umbilical cord blood (50%) and mobilized peripheral blood (10%). Surprisingly, Tie2 receptor expression within the HSC/PC subset was extremely low from both normal and CML samples. Using in vivo transplantation studies, we provide evidence that HLA-DR, c-kitR, Tie2 and IL-3R may not be suitable markers for further partitioning of HSCs from the Lin−CD34+CD38−CD90+CD45RA− sub-population.

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“…8). Therefore, we conclude that human cord blood contains CD34 + /c‐kit <low cells that correspond to mouse P‐HSCs (38).…”

Section: Human P‐hscsmentioning

confidence: 71%

Pluripotent Hemopoietic Stem Cells in Mice and Humans

Ikehara

2000

Proc Soc Exp Biol Med

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Abstract. Although it has been reported previously that pluripotent hemopoietic stem cells (P‐HSCs) express c‐kit, the receptor for stem cell factor (steel factor), we and other groups have recently shown that P‐HSCs do not express c‐kit. In this review, we provide evidence that c‐kit 2 years) and the capacity to form colony‐forming units in spleen (CFU‐S) on Day 16, although c‐kitlow HSCs or c‐kit+ HSCs have LTRA less than 1.5 years and the capacity to form CFU‐S on Day 14 or on Day 10, respectively. In addition, we have found that there is a major histocompatibility complex (MHC) restriction between P‐HSCs and stromal cells; normal P‐HSCs can proliferate and differentiate efficiently in collaboration with MHC class I‐compatible (but not MHC class I‐incompatible) stromal cells. In humans, we also show that c‐kit

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Induction of c‐kit Molecules on Human CD34 ⁺ /c‐kit ^<low Cells: Evidence for CD34 ⁺ /c‐kit ^<low Cells as Primitive Hematopoietic Stem Cells

Cited by 29 publications

References 46 publications

Storage of unprocessed G-CSF-mobilized whole blood in a modified Leibovitz's L15 medium preserves clonogenic capacity for at least 7 days

Storage of unprocessed G-CSF-mobilized whole blood in a modified Leibovitz's L15 medium preserves clonogenic capacity for at least 7 days

Further phenotypic characterization of the primitive lineage− CD34+CD38−CD90+CD45RA− hematopoietic stem cell/progenitor cell sub-population isolated from cord blood, mobilized peripheral blood and patients with chronic myelogenous leukemia

Pluripotent Hemopoietic Stem Cells in Mice and Humans

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Induction of c‐kit Molecules on Human CD34 + /c‐kit <low Cells: Evidence for CD34 + /c‐kit <low Cells as Primitive Hematopoietic Stem Cells

Cited by 29 publications

References 46 publications

Storage of unprocessed G-CSF-mobilized whole blood in a modified Leibovitz's L15 medium preserves clonogenic capacity for at least 7 days

Storage of unprocessed G-CSF-mobilized whole blood in a modified Leibovitz's L15 medium preserves clonogenic capacity for at least 7 days

Further phenotypic characterization of the primitive lineage− CD34+CD38−CD90+CD45RA− hematopoietic stem cell/progenitor cell sub-population isolated from cord blood, mobilized peripheral blood and patients with chronic myelogenous leukemia

Pluripotent Hemopoietic Stem Cells in Mice and Humans

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Resources

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Induction of c‐kit Molecules on Human CD34 ⁺ /c‐kit ^<low Cells: Evidence for CD34 ⁺ /c‐kit ^<low Cells as Primitive Hematopoietic Stem Cells