Induction of CAF-1 Expression in Response to DNA Strand Breaks in Quiescent Human Cells

Nabatiyan, Arman; Szüts, Dávid; Krude, Torsten

doi:10.1128/mcb.26.5.1839-1849.2006

Cited by 41 publications

(38 citation statements)

References 50 publications

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“…Figure 7 shows that PCNA colocalizes partially with both WRN and CAF-1 following HU treatment. This is in agreement with previous reports that PCNA associates with CAF-1 in DNA replication (Moggs et al, 2000), after UV exposure (Green and Almouzni, 2003) process of double-strand breaks repair (Nabatiyan et al, 2006), and that PCNA interacts with WRN during replication (Rodriquez-Lopez et al, 2003). However, how WRN and PCNA coordinately interact with CAF-1 remains to be elucidated.…”

Section: Discussionsupporting

confidence: 92%

The Werner syndrome protein is required for recruitment of chromatin assembly factor 1 following DNA damage

et al. 2006

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The Werner syndrome protein (WRN) and chromatin assembly factor 1 (CAF-1) are both involved in the maintenance of genome stability. In response to DNAdamaging signals, both of these proteins relocate to sites where DNA synthesis occurs. However, the interaction between WRN and CAF-1 has not yet been investigated. In this report, we show that WRN interacts physically with the largest subunit of CAF-1, hp150, in vitro and in vivo. Although hp150 does not alter WRN catalytic activities in vitro, and the chromatin assembly activity of CAF-1 is not affected in the absence of WRN in vivo, this interaction may have an important role during the cellular response to DNA replication fork blockage and/or DNA damage signals. In hp150 RNA-mediated interference (RNAi) knockdown cells, WRN partially formed foci following hydroxyurea (HU) treatment. However, in the absence of WRN, hp150 did not relocate to form foci following exposure to HU and ultraviolet light. Thus, our results demonstrate that WRN responds to DNA damage before CAF-1 and suggest that WRN may recruit CAF-1, via interaction with hp150, to DNA damage sites during DNA synthesis.

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Section: Discussionsupporting

confidence: 92%

The Werner syndrome protein is required for recruitment of chromatin assembly factor 1 following DNA damage

et al. 2006

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“…While it is possible that chromatin remodeling rather than gross chromatin alterations such as chromatin disassembly and reassembly may be sufficient for short patch BER, long patch BER is likely to require chromatin disassembly and reassembly. Accordingly, the histone chaperone CAF-1 colocalizes with the BER machinery in repair foci in cells undergoing base excision repair [61]. This study cleverly examined repair foci in quiescent cells rather than dividing cells in order to ensure that they were not observing foci of CAF-1 due to its role in DNA replication.…”

Section: Chromatin Assembly During Excision Repairmentioning

confidence: 99%

“…Specifically, CAF-1 localizes to repair foci in response to the alkylating agent MNNG [67] and the radiomimetic agent bleomycin [61]. Data has also been published showing that CAF-1 is specifically recruited to immobilized DNA templates following gamma-irradiation [17].…”

Section: Chromatin Disassembly and Assembly During Double-strand Dna mentioning

confidence: 99%

“…The evidence for a role for CAF-1 in NHEJ comes from its colocalization with the NHEJ component XRCC4 in tissue culture cells [61]. Furthermore, the additional inactivation of CAF-1 in a yeast strain that is deleted for RAD52 (disabling homologous recombination) leads to increased DNA damage sensitivity as compared to a strain deleted for RAD52 only [41].…”

Section: Chromatin Disassembly and Assembly During Double-strand Dna mentioning

confidence: 99%

“…Quiescent cells normally only have trace amounts of the CAF-1 protein, but CAF-1 levels are greatly upregulated in response to the radiomimetic agent bleomycin and to ionizing radiation [61]. It is not known whether increased CAF-1 levels after damage are a result of transcriptional induction or stabilization of this normally very unstable protein complex.…”

Section: Regulation Of Histone Chaperones By the Dna Damage Checkpointmentioning

confidence: 99%

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Chromatin disassembly and reassembly during DNA repair

Linger

Tyler

2007

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

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Current research is demonstrating that the packaging of the eukaryotic genome together with histone proteins into chromatin is playing a fundamental role in DNA repair and the maintenance of genomic integrity. As is well established to be the case for transcription, the chromatin structure dynamically changes during DNA repair. Recent studies indicate that the complete removal of histones from DNA and their subsequent reassembly onto DNA accompanies DNA repair. This review will present evidence indicating that chromatin disassembly and reassembly occur during DNA repair and that these are critical processes for cell survival after DNA repair. Concomitantly, candidate proteins utilized for these processes will be highlighted.

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Histone chaperone CAF-1: essential roles in multi-cellular organism development

Liu

Deng

et al. 2014

Cell. Mol. Life Sci.

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More and more studies have shown chromatin remodelers and histone modifiers play essential roles in regulating developmental patterns by organizing specific chromosomal architecture to establish programmed transcriptional profiles, with implications that histone chaperones execute a coordinating role in these processes. Chromatin assembly factor-1 (CAF-1), an evolutionarily conserved three-subunit protein complex, was identified as a histone chaperone coupled with DNA replication and repair in cultured mammalian cells and yeasts. Interestingly, recent findings indicate CAF-1 may have important regulatory roles during development by interacting with specific transcription factors and epigenetic regulators. In this review, we focus on the essential roles of CAF-1 in regulating heterochromatin organization, asymmetric cell division, and specific signal transduction through epigenetic modulations of the chromatin. In the end, we aim at providing a current image of facets of CAF-1 as a histone chaperone to orchestrate cell proliferation and differentiation during multi-cellular organism development.

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Induction of CAF-1 Expression in Response to DNA Strand Breaks in Quiescent Human Cells

Cited by 41 publications

References 50 publications

The Werner syndrome protein is required for recruitment of chromatin assembly factor 1 following DNA damage

The Werner syndrome protein is required for recruitment of chromatin assembly factor 1 following DNA damage

Chromatin disassembly and reassembly during DNA repair

Histone chaperone CAF-1: essential roles in multi-cellular organism development

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