Induction of Cell Cycle Arrest and Apoptotic Response of Head and Neck Squamous Carcinoma Cells (Detroit 562) by Caffeic Acid and Caffeic Acid Phenethyl Ester Derivative

Abstract

Natural polyphenols have been observed to possess antiproliferative properties. The effects, including apoptotic potential of bioactive phenolic compounds, caffeic acid (CA) and its derivative caffeic acid phenethyl ester (CAPE), on cell proliferation and apoptosis in human head and neck squamous carcinoma cells (HNSCC) line (Detroit 562) were investigated and compared. Cancer cells apoptosis rates and cell cycle arrests were analysed by flow cytometry. Exposure to CA and CAPE was found to result in a dose-dependent decrease in the viability of Detroit 562 cells at different levels. CA/CAPE treatment did significantly affect the viability of Detroit 562 cells (MTT results). CAPE-mediated loss of viability occurred at lower doses and was more pronounced, with the concentrations which inhibit the growth of cells by 50% estimated at 201.43 μM (CA) and 83.25 μM (CAPE). Dead Cell Assay with Annexin V labelling demonstrated that CA and CAPE treatment of Detroit 562 cells resulted in an induction of apoptosis at 50 μM and 100 μM doses. The rise of mainly late apoptosis was observed for 100 μM dose and CA/CAPE treatment did affect the distribution of cells in G0/G1 phase. A combination of different phenolic compounds, potentially with chemotherapeutics, could be considered as an anticancer drug.

Antagonistic Effects of CAPE (a Component of Propolis) on the Cytotoxicity and Genotoxicity of Irinotecan and SN38 in Human Gastrointestinal Cancer Cells In Vitro

“…These results correlate with those of Yu et al [45], which demonstrated the ability of CAPE activate caspase-3 and induce apoptosis in YD15, HSC-4 and HN22 cells. Similar results were obtained by Kabała-Dzik et al [46] and Dziedzic et al [47]; CAPE successfully induced apoptosis after 24 h incubation in MDA-MB-231 and NHSCC cell lines. In our work, HT29 was the most resistant to CAPE, which may be due to a mutation in the p53 gene affecting their resistance to apoptosis.…”

Synergistic Anticancer Activity of Combined Use of Caffeic Acid with Paclitaxel Enhances Apoptosis of Non-Small-Cell Lung Cancer H1299 Cells in Vivo and in Vitro

“…Studies have shown that CA has various pharmacological activities, including anti-inflammatory, antioxidant, and immunomodulatory activities [7][8][9]. CA also exhibits an anti-cancer effect against human cervical carcinoma, head and neck squamous carcinoma, colon cancer, and renal carcinoma [12][13][14]26]. A previous study showed that CA promoted NSCLC A549 cell proliferation and enhanced PTX-induced proliferation inA549 cells [17], suggesting that it has the potential to facilitate A549 growth through the p53 pathway and enhance the protective effect of PTX.…”

Caspase Inhibitor Diminishes Caffeic Acid-induced Apoptosis in Osteosarcoma Cells

“…The results also support other previously reported studies of the potential of caffeic in inducing apoptosis in cancer cells. (8,11) In the complex mechanism of apoptosis, caspase plays a very crucial role. In the present study, we found a significant difference in the number of apoptotic cells between caffeic acid-treated group and Z-VAD-pretreated group (p<0.05), in which the percentage of apoptotic cells was lower in the Z-VAD pretreated group.…”

“…(4)(5)(6) Caffeic acid significantly reduces the activity of nuclear factor κB (NFκB), a cell signaling pathway involved in osteoclastogenesis (5), in which NFκB inhibition can cause tumor cells to stop proliferating or becoming more sensitive to the actions of anti-tumor agents. (7) Caffeic acid and related polyphenolic compounds today attract much special attention because of its ability to protect the human body from oxidative stress that can lead to cancer (8), aging (9), and cardiovascular disease (10). A previous study has shown that caffeic acid can induce apoptosis in MG63 osteosarcoma cells and show the presence of caspase-8, -9 and -3 in the induction of apoptosis.…”