Induction of Complete Desiccation Tolerance in Carrot (Daucus carota) Embryoids

Tetteroo, F.A.A.; Hoekstra, Folkert A.; Karssen, C.M.

doi:10.1016/s0176-1617(11)81901-2

Cited by 43 publications

(30 citation statements)

References 32 publications

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“…It was reported previously that slow drying leads to the accumulation of the trisaccharide umbelliferose in carrot somatic embryos (Tetteroo et al, 1994(Tetteroo et al, , 1995. This accumulation does not occur when the embryos are subjected to fast drying.…”

Section: Effect Of Drying Rate On the Composition Of Soluble Carbohydmentioning

confidence: 86%

“…Carrot somatic embryos acquire the capacity to become desiccation tolerant by the addition of ABA to the maturation medium, but the actual tolerance requires slow drying for several days (Tetteroo et al, 1995). Fast drying within a few hours results in poor survival.…”

Section: Discussionmentioning

confidence: 99%

“…Somatic embryos were produced, dried, and germinated as described previously (Tetteroo et al, 1995). Slow and fast drying were carried out as follows.…”

Section: Production Drying and Germination Of Somatic Embryosmentioning

confidence: 99%

“…Before imbibition the embryos inside of the closed Petri dish were prehumidified in moisture-saturated air for 4 h to prevent possible imbibitional damage (Hoekstra et al, 1989). After this treatment, 1 mL of B 5 medium (Gamborg et al, 1968) was added to the embryos (Tetteroo et al, 1995). The Petri dish was sealed with laboratory film (Parafilm, American National Can, Greenwich, CT) and placed in an incubator with a 16 h d Ϫ1 photoperiod at 25°C.…”

Section: Production Drying and Germination Of Somatic Embryosmentioning

confidence: 99%

“…Carrot (Daucus carota) somatic embryos can be rendered tolerant to severe desiccation by a proper combination of treatments (Tetteroo et al, 1994(Tetteroo et al, , 1995(Tetteroo et al, , 1996(Tetteroo et al, , 1998. Addition of ABA at the proper stage of development, a sufficient slow-drying time (at least 4 d), and a subtle rehydration are the main requirements for the acquisition of desiccation tolerance.…”

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confidence: 99%

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Changed Properties of the Cytoplasmic Matrix Associated with Desiccation Tolerance of Dried Carrot Somatic Embryos. An in Situ Fourier Transform Infrared Spectroscopic Study1

Wolkers

Tetteroo²,

Alberda

et al. 1999

Plant Physiology

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Abscisic acid-pretreated carrot (Daucus carota) somatic embryos survive dehydration upon slow drying, but fast drying leads to poor survival of the embryos. To determine whether the acquisition of desiccation tolerance is associated with changes in the physical stability of the cytoplasm, in situ Fourier transform infrared microspectroscopy was used. Although protein denaturation temperatures were similar in the embryos after slow or fast drying, the extent of the denaturation was greater after fast drying. Slowly dried embryos are in a glassy state at room temperature, and no clearly defined glassy matrix was observed in the rapidly dried embryos. At room temperature the average strength of hydrogen bonding was much weaker in the rapidly dried than in the slowly dried embryos. We interpreted the molecular packing to be "less tight" in the rapidly dried embryos. Whereas sucrose (Suc) is the major soluble carbohydrate after fast drying, upon slow drying the trisaccharide umbelliferose accumulates at the expense of Suc. The possibly protective role of umbelliferose was tested on protein and phospholipid model systems, using Suc as a reference. Both umbelliferose and Suc form a stable glass with drying: They depress the transition temperature of dry liposomal membranes equally well, they both prevent leakage from dry liposomes after rehydration, and they protect a polypeptide that is desiccation sensitive. The similar protection properties in model systems and the apparent interchangeability of both sugars in viable, dry somatic embryos suggest no special role of umbelliferose in the improved physical stability of the slowly dried embryos. Also, during slow drying LEA (late-embryogenesis abundant) transcripts are expressed. We suggest that LEA proteins embedded in the glassy matrix confer stability to these slowly dried embryos.Desiccation tolerance is the capacity of an organism or tissue to regain vital metabolism after almost complete dehydration. Seeds, pollens, resurrection plants, mosses and ferns, nematodes, tardigrades, yeasts, fungi spores, and bacteria have this capacity (for review, see Crowe et al., 1992Crowe et al., , 1997aVertucci and Farrant, 1995).Carrot (Daucus carota) somatic embryos can be rendered tolerant to severe desiccation by a proper combination of treatments (Tetteroo et al., 1994(Tetteroo et al., , 1995(Tetteroo et al., , 1996(Tetteroo et al., , 1998. Addition of ABA at the proper stage of development, a sufficient slow-drying time (at least 4 d), and a subtle rehydration are the main requirements for the acquisition of desiccation tolerance. Fast drying within a few hours leads to an almost complete loss of viability. These rapidly dried somatic embryos have a considerably greater leakage of K ϩ and soluble carbohydrates than slowly dried embryos. The excessive leakage of cytoplasmic components upon rehydration is associated with irreversible changes in the plasma membranes. Formation of irreversible protein aggregates and an increased T m have been detected in plasma membranes isolated...

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Section: Effect Of Drying Rate On the Composition Of Soluble Carbohydmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

“…Somatic embryos were produced, dried, and germinated as described previously (Tetteroo et al, 1995). Slow and fast drying were carried out as follows.…”

Section: Production Drying and Germination Of Somatic Embryosmentioning

confidence: 99%