Induction of Osteogenesis in Rat Bone Tissue Using Cryogenically Structured Porous 3D Materials Containing a Bioregulator

Krasnov, M. S.; Shaikhaliev, A. I.; Korshakov, E. V.; Efimenko, M.V.; Soloshenkov, P. P.; Davidova, T. R.; Zvukova, N. D.; Sinitskaya, E. S.; Yamskova, V. P.; Yamskov, I. A.; Lozinsky, V. I.

doi:10.1007/s10517-019-04657-z

Cited by 9 publications

(4 citation statements)

References 11 publications

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“…[49] Due to that the polarized ceramic surface is charged, the negative surface will absorb the positive charged ions in the culture medium to form a positive charged layer. [50] Because the protein molecules and cells are negatively charged, they would be attracted and enriched by the positive surface. The protein layer…”

Section: Biological Propertiesmentioning

confidence: 99%

Preparation and properties of artificial bone with lead-free piezoelectric materials

Cheng¹

2023

MatLab

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In order to fabricate the artificial bone with high bioactive property, lead-free barium titanate (BaTiO3, BT) piezoelectric material added hydroxyapatite (HA) composites were prepared in this study. Compared with the pure HA materials, the introduction of BT can increase the electrical properties of the samples while ensuring good biological properties. The electrical properties, such as piezoelectric constant d33, hysteresis loop and dielectric spectrum were measured, respectively. The bending strength, Vickers hardness, cytotoxicity and osteogenic property of the BT/HA composites were also discussed. It is revealed that the non-toxic sample with 95 wt% BT and 5 wt% HA (95BT+5HA) has the best osteoinductivity, the piezoelectric constant d33 of which is 79.2 pC N-1. The bending strength and Vickers hardness of the 95BT+5HA sample are 138.3 MPa and 472.4, respectively, realizing the desired mechanical properties of human bones. Comprehensive analyses of various properties show that the 95BT+5HA composite can meet the requirements of artificial bone, and is expected to be a promising generation of substitute bone materials.

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Section: Biological Propertiesmentioning

confidence: 99%

Preparation and properties of artificial bone with lead-free piezoelectric materials

Cheng¹

2023

MatLab

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“…are of significance. Both manual measurements and computer-assisted approaches have been used for the mathematical treatment of the images obtained with the aid of various types of microscopy (optical [ 170 , 171 , 172 , 206 , 225 , 230 , 231 , 232 , 233 , 234 , 237 , 238 , 240 , 241 , 243 , 244 , 245 , 246 , 250 , 251 , 252 , 256 , 257 , 258 , 259 , 261 , 262 , 263 , 264 , 265 , 267 , 268 , 269 , 270 , 272 , 380 , 383 , 384 , 390 , 393 ], laser confocal [ 369 , 370 , 371 , 372 , 392 ], and SEM [ 173 , 174 , 177 , 179 , 181 , 182 , 184 , 196 , …”

Section: The Main Effects and Trends Characteristic Of The Cryostrmentioning

confidence: 99%

“…All types of the bioregulator-loaded matrices promoted active bone repair, which manifested in the restoration of the dense bone tissue, formation of the bone marrow, and the recovery of osteons. In contrast, as for the animals that received the implantation of reference sponges without the bioregulator, only the formation of the imperfectly dense fibrous tissue and spongy bone was observed [ 393 ]. The studies in this direction are in progress now.…”

Section: Applied Potential Of Some Cryogenically-structured Polymementioning

confidence: 99%

Cryostructuring of Polymeric Systems. 55. Retrospective View on the More than 40 Years of Studies Performed in the A.N.Nesmeyanov Institute of Organoelement Compounds with Respect of the Cryostructuring Processes in Polymeric Systems

Lozinsky

2020

Gels

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The processes of cryostructuring in polymeric systems, the techniques of the preparation of diverse cryogels and cryostructurates, the physico-chemical mechanisms of their formation, and the applied potential of these advanced polymer materials are all of high scientific and practical interest in many countries. This review article describes and discusses the results of more than 40 years of studies in this field performed by the researchers from the A.N.Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences—one of the key centers, where such investigations are carried out. The review includes brief historical information, the description of the main effects and trends characteristic of the cryostructuring processes, the data on the morphological specifics inherent in the polymeric cryogels and cryostructurates, and examples of their implementation for solving certain applied tasks.

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“…Of the peptide/protein bioregulators of this group, many of them are glycosilated derivatives and consist of peptide-protein complexes with similar physico-chemical properties exhibiting close biological activity [42,43]. The first studies on the use of similar bioregularors loaded in serum-albumin-derived cryogels for the induction of bone tissue regeneration have shown rather promising potential of such delivery systems [44]. In the respective experiments this type bioregulator-loaded sponge has been implanted into the model bone defects in rats thus inducing the restoration of dense bone tissue, the formation of bone marrow, and the recovery of osteons.…”

Section: Introductionmentioning

confidence: 99%

Cryostructuring of Polymeric Systems. 57. Spongy Wide-Porous Cryogels Based on the Proteins of Blood Serum: Preparation, Properties and Application as the Carriers of Peptide Bioregulators

Sidorskii

Krasnov

Yamskova

et al. 2020

Gels

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Wide-pore proteinaceous freeze–thaw spongy gels were synthesized via the cryotropic gelation technique using the bovine blood serum or its diluted solutions as the protein-containing precursors. The feed systems also included the denaturant (urea) and the thiol-reductant (cysteine). The gel-fraction yield decreased and the swelling degree of the walls of macropores in such heterophase matrices increased with decreasing the initial protein concentration. The optimum freezing temperature was found to be within a rather narrow range from −15 to −20 °C. In this case, the average size of the macropores in the resultant cryogels was 90–110 μm. The suitability of such soft wide-pore gel materials for the application as the carriers of peptide bioregulators was demonstrated in the in vitro experiments, when the posterior segments of the Pleurodeles waltl adult newts’ eyes were used as a model biological target. It was shown that a statistically reliable protective effect on the state of the sclera, vascular membrane and retinal pigment epithelium, as well as on the viability of fibroblasts, was inherent in the proteinaceous cryogels loaded with the peptide bioregulator (Viophtan-5™) isolated from the bovine eye sclera.

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Induction of Osteogenesis in Rat Bone Tissue Using Cryogenically Structured Porous 3D Materials Containing a Bioregulator

Cited by 9 publications

References 11 publications

Preparation and properties of artificial bone with lead-free piezoelectric materials

Preparation and properties of artificial bone with lead-free piezoelectric materials

Cryostructuring of Polymeric Systems. 55. Retrospective View on the More than 40 Years of Studies Performed in the A.N.Nesmeyanov Institute of Organoelement Compounds with Respect of the Cryostructuring Processes in Polymeric Systems

Cryostructuring of Polymeric Systems. 57. Spongy Wide-Porous Cryogels Based on the Proteins of Blood Serum: Preparation, Properties and Application as the Carriers of Peptide Bioregulators

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