Induction of settlement in larvae of the mussel Mytilus galloprovincialis using neuroactive compounds

Sánchez-Lazo, Clara; Martínez‐Pita, Inés

doi:10.1016/j.aquaculture.2012.03.021

Cited by 36 publications

(23 citation statements)

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“…Individuals that maintained firm attachment to the substratum were considered settled, and those moving freely with no resistance were considered unsettled. This technique provides a reliable and larvae and is well-established in the literature (Ganesan et al 2010(Ganesan et al , 2012Alfaro et al 2011;Young et al 2011;Sánchez-Lazo et al 2012;Wilkens et al 2012;McDonald et al 2014). Furthermore, we have found 205 this method to be consistently more reliable than other methods (e.g.…”

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confidence: 60%

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Putative involvement of adrenergic receptors in regulation of mussel (Perna canaliculus) larval settlement

Young

Sánchez-Lazo

Robertson

2015

Marine Biology Research

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Settlement responses were investigated for mussel (Perna canaliculus) larvae after exposure to catecholamines and their precursor metabolites. Settlement and mortality assays were conducted in Petri plates with chemical treatments (L-phenylalanine, L-tyrosine, L-DOPA, dopamine hydrochloride and epinephrine at various concentrations) and controls. 15The proteinogenic amino acids L-phenylalanine and L-tyrosine both were effective inducers (~65%) of larval settlement at 10 −5 mol L −1 compared with controls (4%). Exposure of larvae to L-DOPA, dopamine and epinephrine resulted in maximum settlement induction (50, 60 and 51%, respectively) at 10 −5 mol L −1 . Larval mortalities were low (comparable to controls) across all concentrations of L-phenylalanine and L-tyrosine treatments whereas high mortalities (>60%) were observed for L-DOPA, dopamine and epinephrine at concentrations ≥ 10 −4 mol L −1. Our results indicate that P. canaliculus 20 larval settlement is under endogenous regulation by a catecholaminergic mechanism. Furthermore, the inductive effects of all tested metabolites in the epinephrine biosynthesis pathway point to a putative involvement of adrenergic-type receptors in regulation of larval settlement in this mussel species.

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“…Stock solutions (10× concentrates) of each treatment level were prepared by dissolving compounds in 0.45 μm filtered seawater (FSW) 160 immediately prior to settlement assays. These concentrations were chosen based on a pilot study and our experience with other mussel species (Alfaro et al 2011;Sánchez-Lazo et al 2012). .…”

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confidence: 99%

Putative involvement of adrenergic receptors in regulation of mussel (Perna canaliculus) larval settlement

Young

Sánchez-Lazo

Robertson

2015

Marine Biology Research

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“…It is unclear whether this effect was caused by a coupling of the metamorphic gene regulatory network to the induced pre-metamorphic behavior/process prior to the event, or whether acetylcholine is directly involved in the morphogenic signalling pathway during the event. While acetylcholine is a potent inducer of larval settlement in some bivalve species (Yu et al 2008;Alfaro et al 2011;Young et al 2011;Sánchez-Lazo et al 2012), no effect on attachment or metamorphosis has been observed in the five Haliotis spp. tested thus far (H. discus [Fukazawa et al 2001], H. discus hannai [Akashige et al 1981], H. diversilcolor supertexta [Yu et al 2010], H. gigantea [Fukazawa et al 2001], H. rufescens [Morse et al 1979]).…”

Section: Discussionmentioning

confidence: 99%

“…It is unclear whether this effect was caused by a coupling of the metamorphic gene regulatory network to the induced pre-metamorphic behavior/process prior to the event, or whether acetylcholine is directly involved in the 17 morphogenic signalling pathway during the event. While acetylcholine is a potent inducer of larval settlement in some bivalve species (Yu et al 2008;Alfaro et al 2011;Young et al 2011;Sánchez-Lazo et al 2012) Resembling the effects of GABA, KCl, acetylcholine and dopamine, glutamine also reduced swimming activity in H. iris larvae within the first two days of exposure. Although there was some indication of induced attachment at high concentrations (10 -4 mol L -1 ), high mortalities (ca.…”

Section: Discussionmentioning

confidence: 99%

Neurophysiological control of swimming behaviour, attachment and metamorphosis in black-footed abalone (Haliotis iris) larvae

Alfaro

Young

Bowden

2014

New Zealand Journal of Marine and Freshwater Research

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Neurophysiological control of swimming behavior, attachment and metamorphosis in black-footed abalone (Haliotis iris) larvae AbstractExperiments were conducted to test the effect of a range of chemicals on larval responses in swimming behavior, attachment, and metamorphosis of the black-footed abalone (Haliotis iris). The effect of antibiotics on larval survival was first tested within negative (filtered seawater) and positive (GABA at 10 -5 , 10 -4 , and 10 -3 mol L -1 ) control assays over 3days. This experiment corroborated the effectiveness of using antibiotics to improve survival of larvae without obvious synergistic interactions with the GABA inducer or confounding effects of potential bacterial interactions. Chemical treatments (acetylcholine, potassium chloride, dopamine, and glutamine) were then tested at various concentrations for their ability to modulate swimming behavior and induce larval attachment and metamorphosis over 14 days. Generally, larval state shifted from swimming to attached, and from attached to metamorphosed in the control and treatments over time. However, the peak percentage of attached and metamorphosed larvae varied in time among chemicals and concentrations.While overall percent metamorphosis was minimally enhanced after 14 days of exposure to some chemical treatments at certain concentrations, all treatments displayed significant capacities to down-regulate larval swimming and induce early attachment and metamorphosis. Mortality was recorded throughout the duration of the experiment, and was generally low (<20%) across controls and most treatments for exposures of less than 12 days.Interpretations of specific results from this study are used to elucidate neurophysiological control of larval activities for this abalone species. Comparisons with other marine 3 invertebrates highlight the specificities of chemical cues and endogenous regulatory mechanisms across relatively closely related taxa. IntroductionChemoreception is believed to facilitate larval settlement processes and metamorphosis of a great number of marine invertebrate taxa, including polychaetes ( algae where bacterial and diatom biofilms are in abundance. However, the involvement of biofilms in the facilitation of settlement and metamorphosis by crustose coralline algae is questionable for some abalone species (Huggett et al. 2005;Roberts et al. 2007.Furthermore, specific settlement-inducing compounds within these natural surfaces have rarely been identified (but see Morse et al. 1980;Suenaga et al. 2004).Another approach to investigate chemoreception and associated transduction mechanisms has been to test specific pharmacologically active compounds for their ability to induce settlement and metamorphosis. The amino acid and neurotransmitter γ-amino butyric acid (GABA) consistently has been shown to induce larval attachment and metamorphosis of several abalone species (Morse 1990;Roberts & Nicholson 1997; Bryan & Qian 1998; Moss 4 1999). While a pure-form of GABA is unlikely to be an environmental cue (Kaspar & M...

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“…실험에 이용한 신경전달물질은 serotonine (5-HT, sigma USA), gamma amino butyric acid (GABA, sigma USA), et al, 1985;Bonar et al, 1990;Coon et al 1990a,b;Walch et al, 1999;Fang et al, 2001;Kang et al, 2004;García-Lavandeira et al, 2005;Gao and Liu, 2006;Yang et al, 2008;Yang et al, 2011;Sánchez-Lazo et al, 2012;Teh et al, 2012;Mesías-Gansbiller et al, 2013;Yang et al, 2013 (Pawlik, 1990;Couper and Leise, 1996;Leise et al, 2001), 진주조개류 인 Pinctada maxima (Zhao et al, 2003), Pinctada fucata martensii (Yu et al, 2008), Pteria penguin …”

Section: 부착유인 효과mentioning

confidence: 99%

The Effect of Neuroactive Compounds on Settlement of Pacific Oyster, Crassostrea gigas Pediveliger Larvae

Hur¹,

Cho²,

Byun³

et al. 2013

The Korean Journal of Malacology

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We determined the effects of neuroactive compounds known as synthetic larval settlement inducers on the settlement of the Pacific oyster C. gigas pediveliger on the larval collector. Six types of the inducers, serotonin (5-HT), γ-amino butyric acid (GABA), L-3,4-dihydroxyphenylalanine (L-DOPA), norepinephrine, epinephrine and methyl bromide (MB) were tested. All the chemicals induced larval settlement, MB being the most effective with settlement rate of 42.7 ± 2.7%, followed by GABA (35.4 ± 2.0%), 5-HT (29.1 ± 2.2%), L-DOPA (19.2 ± 2.1%), epinephrine (15.2 ± 0.9%), and norepinephrine (11.0 ± 1.2%). The chemicals γ-amino butyric acid and methyl bromide were also better in terms of settled density on the collector with their respective density of 1.97 ± 1.42 and 2.37 ± 1.86 ind/cm 2 , reminiscent of being most effective candidates for a larval settlement inducer in the oyster hatchery. Keywords

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Induction of settlement in larvae of the mussel Mytilus galloprovincialis using neuroactive compounds

Cited by 36 publications

References 43 publications

Putative involvement of adrenergic receptors in regulation of mussel (Perna canaliculus) larval settlement

Putative involvement of adrenergic receptors in regulation of mussel (Perna canaliculus) larval settlement

Neurophysiological control of swimming behaviour, attachment and metamorphosis in black-footed abalone (Haliotis iris) larvae

The Effect of Neuroactive Compounds on Settlement of Pacific Oyster, Crassostrea gigas Pediveliger Larvae

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