Induction of somatic embryogenesis and biochemical characterization of Cordyline australis (G. Forst.) Endl. ‘Red Star’ callus

Warchoł, Marzena; Skrzypek, Edyta; Kusibab, Tadeusz; Dubert, F.

doi:10.1016/j.scienta.2015.05.010

Cited by 14 publications

(12 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During the different stages of somatic embryo's development, no statistical differences were detected in total soluble sugar levels, with mean rates of approximately 70 µg/mg MS. Contrary to results in current study, Gomes et al (2014) and Warchoł et al (2015) reported that during the somatic embryo development of Elaeis guineensis and Cordyline australis, respectively, total soluble sugar concentrations in the cultures' tissues increased gradually, rising to higher rates at the torpedo somatic embryo and regenerating somatic embryo stages, the last two development stages.…”

Section: Soluble Sugarscontrasting

confidence: 99%

“…On the other hand, during the somatic embryo's stages of differentiation and maturing, no statistical differences were registered in total protein levels, with mean rates of approximately 160 µg/mg MS. In C. australis's somatic embryogenesis, Warchoł et al (2015) failed to detect any significant differences in total protein levels during formation and development of somatic embryos. Jariteh al.…”

Section: Total Proteinsmentioning

confidence: 80%

See 1 more Smart Citation

Biochemical events during somatic embryogenesis in Coffea arabica L.

et al. 2018

View full text Add to dashboard Cite

Several biochemical components associated with different stages of somatic embryogenesis in coffee ( L.) are investigated using foliar explants. Soluble sugar, starch, free amino acids and total proteins were extracted and quantified at different stages of somatic embryogenesis, such as foliar segments (initial explants), primary calluses, embryogenic calluses, globular embryos, torpedoes, cotyledonary embryos and mature fruit zygotic embryos. Total soluble sugar levels increased sixfold at the initial stages of somatic embryogenesis induction. During this period, total soluble sugar in the cultures contained approximately 99.3% glucose and fructose. At 67.4 μg/mg MS, no significant changes were observed in total sugar content during the embryo's somatic maturation and regeneration. During this stage, total soluble sugar was composed of 60% sucrose. After primary callus formation, starch contents increased gradually until the culture's conclusion. Total free amino acids, particularly arginine, lysine, methionine, asparagine, glutamine and histidine, revealed a higher synthesis until the formation of the primary callus, after which they remain statistically constant up to the end of the process. During the induction of calluses, a gradual increase of total proteins occurred, which, in the differentiating and maturing of somatic embryos, did not differ statistically till the formation of a cotyledonary embryo, when rates decreased 21.8%.

show abstract

Section: Soluble Sugarscontrasting

confidence: 99%

Section: Total Proteinsmentioning

confidence: 80%

Biochemical events during somatic embryogenesis in Coffea arabica L.

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Throughout the process of regeneration and differentiation of embryogenic calli from the 60 days of cultivation, the amount of sugars in the bacterised embryogenic calli treated with Z78 sonicated cells (T3) significantly increased, achieving as high as 1.96 mg (g FW) -1 for the first 40 days of cultivation, but gradually reduced at D 50 and D 60 . Gomes et al (2014) and Warchol et al (2015) also observed similar results with the increase of total soluble sugar levels during the early stage of maturation for the somatic embryos in Elaeis guineensis and Cordyline australis. The result in this present study can be explained, at least in part, through the rapid cell differentiation that only occurred for the bacterised embryogenic calli with Z78 sonicated cells (T3), whereby the bacterised embryogenic calli started to evolve into compressed embryo, globular and finger like appearance after 30 days of cultivation.…”

Section: Total Sugar Levelssupporting

confidence: 55%

BIOCHEMICAL PROFILE OF BACTERISED CALLI AND EMBRYOGENIC CALLI OF OIL PALM (Elaeis guineensis Jacq.)

Lim¹

2018

JOPR

View full text Add to dashboard Cite

show abstract

“…The auxin NAA was successfully used for somatic embryo germination in many plant species such as Mondia whitei, Bambusa arundinacea and Cordyline australis. 28,29,30 In date palm, NAA was used alone, 21 in combination with BAP, 11 or in combination with other PGRs 18 to induce somatic embryo germination. The findings of the present study showed that combining 5 μM NAA and 5 μM BAP results in the highest somatic embryo germination frequency (26%).…”

Section: Discussionmentioning

confidence: 99%

Impact of Osmotica and Plant Growth Regulators on Somatic Embryogenesis of Date Palm

et al. 2019

View full text Add to dashboard Cite

An efficient somatic embryogenesis system is reported for date palm cv. Al-Fayda, a genotype resistant to the bayoud disease. Callus induction was achieved from adventitious bud explants cultured for 6 months on semi-solid Murashige and Skoog (MS) medium containing 4.5 μM 6-(dimethylallylamino) purine (2iP) and various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) or picloram. The highest somatic embryogenesis frequency (89%) was obtained on MS medium supplemented with 225 μM 2,4-D. Subsequently, embryogenic cultures were transferred to agitated liquid MS medium (maturation medium) containing various concentrations of mannitol, polyethylene glycol (PEG) or sorbitol. The highest rate of somatic embryo maturation (71.4 mature embryos per 100 mg callus) was achieved on the medium supplemented with 40 g l-1 PEG. Mature somatic embryos were then transferred to MS medium supplemented with gibberellic acid (GA3) or 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) at various concentrations. The highest frequency of germination and conversion (26%) was obtained on the medium containing 5 μM NAA and 5 μM BAP. The developed plants were then transferred to ex vitro conditions, where a survival rate of 77.02% was observed. The regeneration protocol established in the present investigation will be used for mass propagation of date palm cv. Al-Fayda.

show abstract

Induction of somatic embryogenesis and biochemical characterization of Cordyline australis (G. Forst.) Endl. ‘Red Star’ callus

Cited by 14 publications

References 42 publications

Biochemical events during somatic embryogenesis in Coffea arabica L.

Biochemical events during somatic embryogenesis in Coffea arabica L.

BIOCHEMICAL PROFILE OF BACTERISED CALLI AND EMBRYOGENIC CALLI OF OIL PALM (Elaeis guineensis Jacq.)

Impact of Osmotica and Plant Growth Regulators on Somatic Embryogenesis of Date Palm

Contact Info

Product

Resources

About