Induction of Somatic Embryos from Different Explants of Citrus sinensis

Kiong, Anna Ling Pick; Wan, Leong Sok; Hussein, Sobri; Ibrahim, Rusli

doi:10.3923/jps.2008.18.32

Cited by 5 publications

(2 citation statements)

References 37 publications

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“…Callus induction frequencies changed with the variation of hormonal concentrations in the same medium. Maximum frequency was 86.7% ± 3.4% in MSCIM media when supplemented with 3 mg/l 2,4-D, 83.3% ± 8.8% in N6CIM media when supplemented with 2.5 mg/l 2,4-D and 82.2% ± 1.9% in B5CIM medium when supplemented with 3.5 mg/l 2,4-D. Nearly similar results were found in previous reports [16] [21] [22]. Supplementation of NAA also showed varied results in callus induction but the frequency of callus induction was relatively lower than 2,4-D.…”

Section: Resultssupporting

confidence: 90%

“…The present research was carried out to develop a reproducible regeneration protocol for Citrus sinensis (L.) Osbeck from mature seed-derived calli. According to previous reports, supplementation of 2,4-D in the medium induce a high percentage of callus in Citrus [16]. High-frequency callus initiation has also been achieved using NAA [17] and also the combination of 2,4 D and NAA [18].…”

Section: Resultsmentioning

confidence: 99%

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In-Vitro Regeneration of Citrus sinensis (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

Hasan¹,

Hasan²,

Foysal³

et al. 2019

AJPS

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In-vitro callus induction and regeneration method was developed using different plant growth regulators (PGRs), and basal media (Murashige and Skoog (MS), CHU (N6) and Gamborg (B5) media) of Citrus sinensis (L.) Osbeck. Observations of the effect of PGRs were carried out using different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D),1-naphthalene acetic acid (NAA) and combinations of 2,4-D and NAA using different basal media. This study found Citrus sinensis (L.) Osbeck exhibited a high frequency of callus induction on MS medium supplemented with 3 mg/L 2,4-D and callus induction frequency was 86.7% ± 3.4% whereas N6 and B5 showed lower callus induction frequency of 83.3% ± 8.8% and 82.2% ± 1.9% respectively compared to that of MS media with supplementation of the same hormone. Among the induced calli, the morphological analysis showed only 40% -50% was embryogenic calli. Regeneration of plantlets from calli was done using different concentrations and combinations of auxin and cytokinin. The study showed that 3 mg/L 6-benzylaminopurine (BAP) supplemented medium has the maximum potential to promote regeneration of Citrus sinensis (L.) Osbeck from embryogenic calli with the frequency of 89.3% ± 8.8% but no regeneration occurred from the non-embryogenic calli. The regenerated plantlets were rooted on MS medium with supplementation of 5 mg/l NAA.These observations in Citrus sinensis (L.) Osbeck regeneration will be helpful for genetic improvement with desired traits.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

In-Vitro Regeneration of Citrus sinensis (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

Hasan¹,

Hasan²,

Foysal³

et al. 2019

AJPS

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Somatic Embryogenesis, In Vitro Selection and Plantlet Regeneration for Citrus Improvement

Kumar

Gill

Gosal

2018

Biotechnologies of Crop Improvement, Volume 1

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Development of Efficient Callus Initiation of Malta (Citrus sinensis) Through Tissue Culture

Azim

Rahman

Prodhan

et al. 2013

Int. J. Agril. Res. Innov. & Tech.

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The effects of different hormonal concentration on shoot formation and callus induction were studied on BARI Malta-1 (Citrus sinensis). Seeds containing seed coat and without seed coat was treated by Murashige and Skoog (MS) media supplemented with 6-benzyl adenine (BA) and Kinetin (KIN). Removal of seed coat showed an early response for shoot formation. The highest (70%) shoot formation was obtained from seeds without seed coat treated with MS basal media + BA 1.0 mg/l while KIN showed no response for shoot formation in any supplemented concentration. However, in case of callus induction internodes and apical shoot tips were used as explants and 2, 4-dichlorophenoxy acetic acid (2, 4-D) was used as callus inducing hormone. MS basal media supplemented with 2, 4-D, 2.0 mg/l showed highest (68%) callus induction.

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Induction of Somatic Embryos from Different Explants of Citrus sinensis

Cited by 5 publications

References 37 publications

<i>In-Vitro</i> Regeneration of <i>Citrus sinensis</i> (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

<i>In-Vitro</i> Regeneration of <i>Citrus sinensis</i> (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

Somatic Embryogenesis, In Vitro Selection and Plantlet Regeneration for Citrus Improvement

Development of Efficient Callus Initiation of Malta (Citrus sinensis) Through Tissue Culture

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Induction of Somatic Embryos from Different Explants of Citrus sinensis

Cited by 5 publications

References 37 publications

&lt;i&gt;In-Vitro&lt;/i&gt; Regeneration of &lt;i&gt;Citrus sinensis&lt;/i&gt; (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

&lt;i&gt;In-Vitro&lt;/i&gt; Regeneration of &lt;i&gt;Citrus sinensis&lt;/i&gt; (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

Somatic Embryogenesis, In Vitro Selection and Plantlet Regeneration for Citrus Improvement

Development of Efficient Callus Initiation of Malta (Citrus sinensis) Through Tissue Culture

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Product

Resources

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<i>In-Vitro</i> Regeneration of <i>Citrus sinensis</i> (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

<i>In-Vitro</i> Regeneration of <i>Citrus sinensis</i> (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media