Induction of stable linkage between the deoxyribonucleic acid dependent ribonucleic acid polymerase and d(A-T)<sub>n</sub>·d(A-T)<sub>n</sub> by ultraviolet light

Strniste, G.F.; Smith, David A.

doi:10.1021/bi00700a014

Cited by 51 publications

(18 citation statements)

References 41 publications

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“…This tends to support the idea that primary photoreactions are responsible for the observed cross-linking (see Section 4). Strniste and Smith (1974) also presented evidence, based on CsCI equilibrium density gradient experiments, that the cross-linking can involve either the u or a subunit of the RNA polymerase.…”

Section: 22b Rna Polymerasementioning

confidence: 97%

“…Strniste and Smith (1974) studied the photoinduced binding of E. coli RNA polymerase to poly d(A-T)n:d(A-T)n' while Hillel and Wu (1978) have scrutinized the UVinduced cross-linking of E. coli RNA polymerase to DNA from T7 phage. Strniste and Smith (1974) used filter-binding assays and CsCI sedimentation equilibrium gradients to study the UV -induced cross-linking of RNA polymerase to poly d(A-T)n: d(A-T)n. They found that irradiation with a UV germicidal lamp led to a complex that was stable to high salt concentrations, to KOH at a pH of 12.6 for 1 hr, and to heating at 93°C for 15 mins. The irradiation of poly d(A-T)n:d(A-T)n and RNA polymerase separately, following by mixing, did not lead to the formation of complexes stable to high salt concentrations.…”

Section: 22b Rna Polymerasementioning

confidence: 99%

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Cross-Linking of Proteins to Nucleic Acids by Ultraviolet Light

Shetlar

1980

Photochemical and Photobiological Reviews

100

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Section: 22b Rna Polymerasementioning

confidence: 97%

Section: 22b Rna Polymerasementioning

confidence: 99%

Cross-Linking of Proteins to Nucleic Acids by Ultraviolet Light

Shetlar

1980

Photochemical and Photobiological Reviews

100

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“…A variety of proteins cross-link to DNA or polynucleotides upon irradiation by UV light; these include, for example, histones (6)(7)(8)(9)(10)(11)(12)(13), Escherichia coli DNA polymerase I (14), E. coli RNA polymerase (15)(16)(17)(18), fd phage gene-5 protein (19), single-stranded DNA binding protein from E. coli (20), four of the component proteins of the DNA replication complex of T4 phage (21), A1 heterogeneous nuclear ribonucleoprotein (22), E. coli uracil (Ura)-DNA glycosylase (23) and rat DNA polymerase ␤ (24,25).…”

Section: Introductionmentioning

confidence: 99%

Ring Opening Photoreactions of Cytosine and Uracil with Ethylamine

Hom¹,

Strahan²,

Shetlar³

2007

Photochemistry and Photobiology

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The photochemical reactions of cytosine (Cyt) and uracil (Ura) with ethylamine, an analog of the side chain of the amino acid lysine, have been studied. After irradiation of Cyt in aqueous ethylamine at ‫؍‬ 254 nm, N-(N-ethylcarbamoyl)-3-aminoacrylamidine (Ia) and N-(N-ethylcarbamoyl)-3-ethylaminoacrylamidine (Ib) were isolated as products, while irradiation of Ura gave N-(N-ethylcarbamoyl)-3-aminoacrylamide (IIa) and N-(N-ethylcarbamoyl)-3-ethylaminoacrylamide (IIb) as products. Studies in which Ia and IIa were incubated with ethylamine at various pH values indicate that Ib and IIb are secondary products produced via thermal reactions of Ia and IIa with ethylamine. Heating of Ia and Ib leads to ring closure with the resultant formation of 1-ethylcytosine; small amounts of 1-ethyluracil are also produced. Heating of IIa and IIb produces 1-ethyluracil as the sole product. Spectroscopic properties were determined for each of these opened ring products, as well as for N-(N-ethylcarbamoyl)-3-amino-2-methylacrylamidine (III) and N-(N-ethylcarbamoyl)-3-amino-2-methylacrylamide (IV). Quantum yield measurements showed that Ia was formed with a ⌽ of 1.6 ؋ 10 Ϫ4 at pH 9.8, while ⌽ for formation of IIa was 7.2 ؋ 10 Ϫ4 at pH 11.5. A profile of the relative quantum yield for formation of Ia, determined as a function of pH, showed that the maximum quantum yield occurs at around pH 9.5; the analogous profile for IIa shows a maximum quantum yield at pH 11.3 and above. Acetone sensitization does not produce Ia in the Cyt-ethylamine system, which indicates that the known triplet state of Cyt is not involved in reactions leading to this opened ring product.

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“…It was shown previously that ultraviolet light can induce the formation of crosslinks between DNA and protein (14). More recently the formation of stable DNA polymerase-DNA adducts was reported (15) and also of RNA polymerase-poly [d(A-T)j (16). In the latter complex the covalent crosslinking was demonstrated by its resistance to dissociate in high salt concentration or by treatment with moderate alkali or by heating.…”

Section: C) Information Retrieval Limited 1 Falconberg Court London Wmentioning

confidence: 99%

Subunit topography of RNA polymerase (E. coli) in the complex with DNA

Okada¹,

Vergne²,

Brahms³

1978

Nucl Acids Res

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E. Coli RNA polymerase binding to different DNAs (from E. Coli, 5-bromodeoxyuridine (BrdUrd) substituted DNA and poly [d(BrU-A)] was induced with ultraviolet (U.V.) light to form protein-DNA crosslinked complexes. Two independent methods of analysis, polyacrylamide gel electrophoresis in SDS and chloroform extraction indicated the formation of a stable complex between the enzyme and DNA. The complexes were formed under different ionic strength conditions, at low enzyme to DNA ratios in order to approach the conditions of specific binding. In contrast there was no crosslinking of the complex in 1 M KCl solution which dissociates the enzyme from DNA. The efficiency of formation of strongly bound complex was found to be much higher with holoenzyme than with core enzyme. The following results were obtained : 1) The large subunits beta and beta' were found to be bound to DNA. 2) Relatively small amount of sigma subunit were bound to DNA while alpha subunits were essentially not attached to DNA. The high binding affinity of beta and beta' subunits was also observed in the studies of isolated subunits. These results lead to a model of enzyme-DNA complex in which the large beta and beta' subunits provide the contacts between the RNA polymerase and the DNA.

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Induction of stable linkage between the deoxyribonucleic acid dependent ribonucleic acid polymerase and d(A-T)_n·d(A-T)_n by ultraviolet light

Cited by 51 publications

References 41 publications

Cross-Linking of Proteins to Nucleic Acids by Ultraviolet Light

Cross-Linking of Proteins to Nucleic Acids by Ultraviolet Light

Ring Opening Photoreactions of Cytosine and Uracil with Ethylamine

Subunit topography of RNA polymerase (E. coli) in the complex with DNA

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Induction of stable linkage between the deoxyribonucleic acid dependent ribonucleic acid polymerase and d(A-T)n·d(A-T)n by ultraviolet light

Cited by 51 publications

References 41 publications

Cross-Linking of Proteins to Nucleic Acids by Ultraviolet Light

Cross-Linking of Proteins to Nucleic Acids by Ultraviolet Light

Ring Opening Photoreactions of Cytosine and Uracil with Ethylamine

Subunit topography of RNA polymerase (E. coli) in the complex with DNA

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Product

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Induction of stable linkage between the deoxyribonucleic acid dependent ribonucleic acid polymerase and d(A-T)_n·d(A-T)_n by ultraviolet light