Industrial Processes Using Biocatalysts

Kleinbeck, Florian; Mahut, Marek; Schlama, Thierry

doi:10.1002/9783527824465.ch15

Cited by 2 publications

(1 citation statement)

References 58 publications

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“…Being enzymes composed of a sequence of (chiral) amino acids, the actual place in which (bio)catalysis occurs -the so-called enzyme's active siteresults to be a multi-chiral cavity, in which enantio-, regioand chemo-selective reactions take place. This is particularly important for pharmaceutical chemistry, where highly functionalized molecules demanding delicate approaches are frequently targeted [12][13][14][15][16][17][18][19] The set-up of a biocatalytic process requires interdisciplinary research, covering areas like molecular biology, organic synthesis, process engineering, bioinformatics, and market analysis. This ensures the holistic management of the entire pipeline, from enzyme discovery to scale-up and market penetration (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Biocatalysis for the asymmetric synthesis of Active Pharmaceutical Ingredients (APIs): this time is for real

Gonzalo

Alcántara

Marı́a³

et al. 2022

Expert Opinion on Drug Discovery

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Introduction: Biocatalysis has emerged as a powerful and useful strategy for the synthesis of active pharmaceutical ingredients (APIs). The outstanding developments in molecular biology techniques allow nowadays the screening, large-scale production, and designing of biocatalysts, adapting them to desired reactions. Many enzymes can perform reactions both in aqueous and non-aqueous media, broadening even further the opportunities to integrate them in complex pharmaceutical multi-step syntheses.Areas covered: This paper showcases several examples of biocatalysis in the pharmaceutical industry, covering examples of different enzymes, such as lipases, oxidoreductases, and transaminases, to deliver active drugs through complex synthetic routes. Examples are critically discussed in terms of reaction conditions, motivation for using an enzyme, and how biocatalysts can be integrated in multi-step syntheses. When possible, biocatalytic routes are benchmarked with chemical reactions. Expert Opinion: The reported enzymatic examples are performed with high substrate loadings (>100 g L −1 ) and with excellent selectivity, making them inspiring strategies for present and future industrial applications. The combination of powerful molecular biology techniques with the needs of the pharmaceutical industry can be aligned, creating promising platforms for synthesis under more sustainable conditions.

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Section: Introductionmentioning

confidence: 99%

Biocatalysis for the asymmetric synthesis of Active Pharmaceutical Ingredients (APIs): this time is for real

Gonzalo

Alcántara

Marı́a³

et al. 2022

Expert Opinion on Drug Discovery

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A Chemoenzymatic Hydroaminomethylation Strategy for the Selective Synthesis of Linear Primary Amines from Olefins by Sequential and Tandem Processes

Manske,

Nicolai,

Bloomer

et al. 2024

ACS Catal.

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Methods that form amines from feedstock chemicals are an important component of industrial chemistry. Many methods for the synthesis of amines yield mixtures of primary, secondary, and tertiary amines, necessitating costly downstream separations. We present a chemoenzymatic approach to hydroaminomethylation that addresses these challenges by combining hydroformylation catalyzed by a phosphine-bound rhodium complex with enzymatic transamination catalyzed by an ω-transaminase from Vibrio fluvialis (VfTA). This sequential chemoenzymatic hydroaminomethylation reaction converts olefins to linear primary amines with high regioselectivity and chemoselectivity for the linear primary amine, and we demonstrate that this process occurs with a series of olefins with varying structures. Furthermore, we report progress toward a tandem process that incorporates a biocatalytic cascade for recycling an intermediate amine donor, which is required for the transaminase enzyme to use an ammonium salt as the terminal nitrogen source. This study illustrates the potential to combine chemo- and biocatalytic reactions to produce valuable materials from readily available feedstocks in both tandem and sequential processes, with selectivities that have not been achieved with transition-metal catalysts.

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Industrial Processes Using Biocatalysts

Cited by 2 publications

References 58 publications

Biocatalysis for the asymmetric synthesis of Active Pharmaceutical Ingredients (APIs): this time is for real

Biocatalysis for the asymmetric synthesis of Active Pharmaceutical Ingredients (APIs): this time is for real

A Chemoenzymatic Hydroaminomethylation Strategy for the Selective Synthesis of Linear Primary Amines from Olefins by Sequential and Tandem Processes

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