Infecção de sementes de trigo com Bipolaris sorokiniana pela técnica de restrição hídrica

Farias, C. R. J. de; Ponte, Emerson M. Del; Corrêa, Carla Lima; Afonso, Ana Paula; Pierobom, Carlos R.

doi:10.1590/s1982-56762010000400009

Cited by 6 publications

(5 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar results were observed in cotton, rice, sunflower, and common bean seeds [53,56,57]. Several studies reported that the incubation period to obtain inoculated seeds ranged between 24 and 144 h [58,59,60,61].…”

Section: Discussionsupporting

confidence: 76%

Molecular Detection of the Seed-Borne Pathogen Colletotrichum lupini Targeting the Hyper-Variable IGS Region of the Ribosomal Cluster

Pecchia

Caggiano

Lio

et al. 2019

Plants

View full text Add to dashboard Cite

Lupins anthracnose is a destructive seed and airborne disease caused by Colletotrichum lupini, affecting stems and pods. Primary seed infections as low as 0.01–0.1% can cause very severe yield losses. One of the most effective management strategies is the development of a robust and sensitive seed detection assay to screen seed lots before planting. PCR-based detection systems exhibit higher levels of sensitivity than conventional techniques, but when applied to seed tests they require the extraction of PCR-quality DNA from target organisms in backgrounds of saprophytic organisms and inhibitory seed-derived compounds. To overcome these limitations, a new detection protocol for C. lupini based on a biological enrichment step followed by a PCR assay was developed. Several enrichment protocols were compared with Yeast Malt Broth amended with ampicillin, streptomycin, and lactic acid were the most efficient. A species-specific C. lupini primer pair was developed based on rDNA IGS sequences. The specificity was evaluated against 17 strains of C. lupini, 23 different Colletotrichum species, and 21 different organisms isolated from seeds of Lupinus albus cv. Multitalia, L. luteus cv. Mister, and L. angustifolius cv. Tango. The protocol described here enabled the detection of C. lupini in samples artificially infected with less than 1/10,000 infected seed.

show abstract

Section: Discussionsupporting

confidence: 76%

Molecular Detection of the Seed-Borne Pathogen Colletotrichum lupini Targeting the Hyper-Variable IGS Region of the Ribosomal Cluster

Pecchia

Caggiano

Lio

et al. 2019

Plants

View full text Add to dashboard Cite

show abstract

“…In short, 24 hours proved to be long enough for P. tritici-repentis infection, penetrating easily through the protective tissues of wheat seeds. Farias et al (2010) found similar results studying wheat seeds at different water potentials and inoculation times, noting that infection by B. sorokiniana was induced with an average efficiency of 53 to 92%. Several other studies have shown that infection efficiency depends on the pathogen species.…”

Section: Exposure Time Of Wheat Seeds To P Tritici-repentis At Diffesupporting

confidence: 68%

“…Water stress is used to control or inhibit seed germination under different circumstances such as in health tests on paper substrate (CELANO et al, 2012) and potato dextrose agar medium (PDA) (GARCIA JÚNIOR et al, 2008), and in fungal inoculation tests (FARIAS et al, 2010), besides simulating water deficit (GIROTTO et al, 2012), osmotic and matric conditioning (QUEIROGA et al, 2011) for crops of interest. A water restriction condition can be obtained by adjusting water potential with osmotically active solutes, with the most common being mannitol, polyethylene glycol, maltose (GIROTTO et al, 2012), KCl, NaCl, sucrose (GARCIA JÚNIOR et al, 2008), or CaCl 2 (MACHADO NETO et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Water potential and time of Pyrenophora tritici-repentis inoculation in wheat seeds

Bertagnolli

Deuner

Frizon

et al. 2017

SCA

View full text Add to dashboard Cite

Wheat seeds infected with Pyrenophora tritici-repentis, the causal agent of tan spot, are partially responsible for outbreaks caused by this pathogen. Seed lots with a high incidence of P. tritici-repentis must be rapidly acquired for transmission and pathogen-control studies. Therefore, the aim of this study was to determine whether changes in water potential of culture medium and variations in inoculation time might favor the infection of wheat seeds by P. tritici-repentis without compromising seed viability. Colonies of P. tritici-repentis were grown on potato-dextrose-agar (PDA) culture medium, adjusted to a water potential of -0.36 MPa, under water stress induced by mannitol at potentials of -0.4, -0.6, -0.8, -1.0, and -1.2 MPa. Analyses were carried out to determine mycelial growth index and seed exposure time to the culture medium to start germination as a function of water potential. Afterwards, wheat seeds were placed in contact with colonies of P. tritici-repentis for 24, 48, and 72 hours at potentials of -0.4, -0.6, -0.8, -1.0, and -1.2 MPa. The experiment was arranged in a completely randomized design, in a factorial scheme (water potential × inoculation time). Rates of germination, seedling emergence in soil, and seed infection were assessed. Mycelial growth was stimulated at lower water potentials, which germinated faster. A 24-hour inoculation time and a -0.4 MPa water potential were efficient to infect wheat seeds with P. tritici-repentis, without hindering seedling germination and emergence under laboratory conditions.

show abstract

“…Lotes contaminados nem sempre estão disponíveis prontamente ou contém o tipo de patógeno e os níveis desejados de infecção, dificultando os estudos no âmbito da patologia de sementes (FARIAS et al, 2010). Contudo, com a modificação do potencial osmótico do substrato, técnica esta denominada restrição hídrica (COUTINHO et al, 2001;MACHADO;LANGERAK, 2002;MACHADO et al, 2008) é possível promover diferentes níveis de infecção por fungos nas sementes, além de inibir a germinação das mesmas (MACHADO et al, 2004, ARAUJO et al, 2006.…”

Section: Introductionunclassified

Danos causados por diferentes potenciais de inóculo de Aspergillus ochraceus no vigor de sementes de soja

et al. 2014

View full text Add to dashboard Cite

ResumoA incidência e a severidade de Aspergillus ochraceus pode reduzir a qualidade fisiológica das sementes. Neste trabalho, objetivou-se estudar a qualidade fisiológica de sementes de soja naturalmente infectadas com A. ochraceus. Sementes de soja cv. Conquista com diferentes níveis de inóculo de A. ochraceus foram obtidas por meio da técnica de restrição hídrica e separadas em três níveis (N0, N1 e N2) de acordo com a área superfícial coberta com estruturas fúngicas. Para compor as testemunhas foram retiradas do tratamento N0 duas amostras de 200 sementes de soja, sendo a primeira tratada com os fungicidas carboxin + thiram e a segunda amostra submetida à desinfestação superficial por imersão em solução de hipoclorito de sódio. A seguir, avaliou-se o vigor das sementes por meio do teste de emergência em bandejas e pelo teste de tetrazólio. As avaliações do comprimento de plântulas, massa fresca e massa seca da parte aérea e da raiz foram realizadas aos 21 dias após a semeadura. Observou-se redução do índice de velocidade de emergência e da emergência de plântulas com níveis de até 50% de inóculo em comparação ao tratamento com fungicida. O comprimento das plântulas (44%), a massa fresca da parte aérea e da raiz (82%), a massa seca da parte aérea (81%) e a massa seca da raiz (76%) também foram reduzidos. Sementes de soja com incidência acima de 50% de A. ochraceus apresentaram perda total do vigor e deterioração dos tecidos. Palavras-chave: Deterioração de semente, fungos de armazenamento, Glycine max AbstractThe incidence and severity of Aspergillus ochraceus can reduce seed quality. In this study, we aimed to study the physiological quality of soybean seeds naturally infected with A. ochraceus. Seeds of soybean cv. Conquest with different levels of inoculum of A. ochraceus were obtained by the technique of water and separated into three classes (N0, N1 and N2) according to the constraint surface area covered with fungal structures. The control samples were taken from the N0 treatment, two samples of 200 soybean seeds, the first being treated with fungicides carboxin + thiram and the second sample subjected to surface disinfestation by immersion in a solution of sodium hypochlorite. Next, we assessed the vigour of seeds through the emergency test in trays and the tetrazolium test. The length, seedling fresh and dry weight of shoot and root were evaluated at 21 days after sowing. There was a reduction in the rate

show abstract

Infecção de sementes de trigo com Bipolaris sorokiniana pela técnica de restrição hídrica

Cited by 6 publications

References 8 publications

Molecular Detection of the Seed-Borne Pathogen Colletotrichum lupini Targeting the Hyper-Variable IGS Region of the Ribosomal Cluster

Molecular Detection of the Seed-Borne Pathogen Colletotrichum lupini Targeting the Hyper-Variable IGS Region of the Ribosomal Cluster

Water potential and time of Pyrenophora tritici-repentis inoculation in wheat seeds

Danos causados por diferentes potenciais de inóculo de Aspergillus ochraceus no vigor de sementes de soja

Contact Info

Product

Resources

About