Infection dynamics and genetic variability of Mycoplasma hyopneumoniae in self-replacement gilts

Takeuti, Karine Ludwig; Barcellos, David Emílio Santos Neves de; Andrade, Caroline Pinto de; Almeida, Laura Lopes de; Pieters, Maria

doi:10.1016/j.vetmic.2017.07.007

Cited by 18 publications

(13 citation statements)

References 21 publications

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“…hyopneumoniae infection dynamics in which piglets were also negative at weaning (Takeuti et al, 2017a(Takeuti et al, , 2017b.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies described a high intra-farm genetic diversity (Stakenborg., 2006;Nathues et al, 2011;dos Santos et al, 2015;Tamiozzo et al, 2015;Michiels et al, 2017;Takeuti et al, 2017a) whereas others concluded that the variability was limited within the same farm (Stakenborg et al, 2005;Mayor et al, 2007Mayor et al, , 2008Vranckx et al, 2012;Charlebois et al, 2014;Galina-Pantoja L. et al, 2016). Results from the current study showed high M. hyopneumoniae variability within the farm.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of vaccination protocols against Mycoplasma hyopneumoniae during the gilt acclimation period

Garza-Moreno

Pieters

López-Soria

et al. 2019

Veterinary Microbiology

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This study evaluated different gilt vaccination protocols against Mycoplasma (M.) hyopneumoniae at acclimation and their effect on the genetic diversity. A total of 180 M. hyopneumoniae naïve gilts were selected 1 week post-entry (wpe) at the acclimation barn in a clinically affected M. hyopneumoniae farm. Gilts were distributed according to the M. hyopneumoniae antibodies levels into three different vaccination schedules: A) four doses of a M. hyopneumoniae commercial vaccine at 2, 4, 6 and 8 wpe; B) two vaccine doses at 2 and 6 wpe and PBS at 4 and 8 wpe; and C) four PBS doses at the same wpe. Detection of M. hyopneumoniae (rt-PCR) and antibodies (ELISA) were assessed in gilts at 1, 14, 27 and 34 wpe and in 6 of their piglets at weaning. rt-PCR positive gilts were detected at 14 wpe, being the proportion significantly lower in groups A and B (3/120, 3%) than C (27/60, 45%). Seroconversion was detected at 14 wpe, showing significant differences in percentage of inhibition (PI) between groups A (median 4.9, range 3.1-19.9) and B (5.5, 3.7-13.5), and C (14.3, 3.3-53.2). Gilts remained seropositive over the study; significant differences in PI were detected between groups A and B versus C. Piglets were rt-PCR negative; proportion of seropositive piglets coming from vaccinated gilts was significantly higher than the non-vaccinated group. M. hyopneumoniae characterization showed high variability. Hence, gilt vaccination with 2 or 4 doses significantly decreased the pathogen infectious pressure, variability, and provided high antibody levels to gilts and their offspring.

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“…hyopneumoniae infection dynamics in which piglets were also negative at weaning (Takeuti et al, 2017a(Takeuti et al, , 2017b.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparison of vaccination protocols against Mycoplasma hyopneumoniae during the gilt acclimation period

Garza-Moreno

Pieters

López-Soria

et al. 2019

Veterinary Microbiology

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“…As identified in Mycoplasma spp., house‐keeping genes have been described to be highly conserved, resulting in limited variation among strains (Dumke, Catrein, Pirkil, Herrmann, & Jacobs, ). The MLVA assay is a typing method that identifies the number of VNTRs within surface proteins and has been employed for the molecular characterization of M. hyopneumoniae in Spain, Argentina, Belgium, United States, Mexico and Brazil (Table ; de Castro et al, ; Michiels et al, ; Dos Santos et al, ; Takeuti et al, ; Tamiozzo et al, ; Vranckx et al, ). Several techniques have described the M. hyopneumoniae variability using different loci numbers (e.g.…”

Section: Molecular Characterization Methods Of Mycoplasmasmentioning

confidence: 99%

“…Furthermore, in swine dense geographical areas, multiple M. hyopneumoniae VNTR types have been identified in endemically infected herds and even in individual pigs (Michiels et al, ; Nathues et al, ; Vranckx et al, ). Greater homogeneity and/or identical M. hyopneumoniae VNTR types in herds that are of close geographical proximity or herds that belong to the same production flow, have also been shown (Charlebois et al, ; Mayor, Zeeh, Frey, & Kuhnert, ; Pantoja, Pettit, Santos, Tubbs, & Pieters, ; Rebaque et al, ; Takeuti et al, ). In all cases, one distinct M. hyopneumoniae VNTR type along with multiple clonal types (i.e.…”

Section: Mycoplasma Hyopneumoniae Variabilitymentioning

confidence: 95%

“…temporal changes) for M. hyopneumoniae genomic heterogeneity across different geographical locations has not been investigated. Several M. hyopneumoniae VNTR types have been identified (Vranckx et al, ) within a country and individual state, suggesting a wide circulation of M. hyopneumoniae VNTR types in swine populations (Dos Santos et al, ; Takeuti, Barcellos, Andrade, Almeida, & Pieters, ; Vranckx et al, ). Among all VNTR types detected in different countries (i.e.…”

Section: Mycoplasma Hyopneumoniae Variabilitymentioning

confidence: 99%

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Mycoplasma hyopneumoniaevariability:Current trends and proposed terminology for genomic classification

Betlach

Maes

Garza-Moreno

et al. 2019

Transbound Emerg Dis

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Mycoplasma hyopneumoniae (M. hyopneumoniae) is the aetiologic agent of enzootic pneumonia in swine, a prevalent chronic respiratory disease worldwide. Mycoplasma hyopneumoniae is a small, self-replicating microorganism that possesses several characteristics allowing for limited biosynthetic abilities, resulting in the fastidious, hostspecific growth and unique pathogenic properties of this microorganism. Variation across several isolates of M. hyopneumoniae has been described at antigenic, proteomic, transcriptomic, pathogenic and genomic levels. The microorganism possesses a minimal number of genes that regulate the transcription process. Post-translational modifications (PTM) occur frequently in a wide range of functional proteins. The PTM by which M. hyopneumoniae regulates its surface topography could play key roles in cell adhesion, evasion and/or modulation of the host immune system. The clinical outcome of M. hyopneumoniae infections is determined by different factors, such as housing conditions, management practices, co-infections and also by virulence differences among M. hyopneumoniae isolates. Factors contributing to adherence and colonization as well as the capacity to modulate inflammatory and immune responses might be crucial. Different components of the cell membrane (i.e. proteins, glycoproteins and lipoproteins) may serve as adhesins and/or be toxic for the respiratory tract cells. Mechanisms leading to virulence are complex and more research is needed to identify markers for virulence. The utilization of typing methods and complete or partial-gene sequencing for M. hyopneumoniae characterization has increased in diagnostic laboratories as control and elimination strategies for this microorganism are attempted worldwide. A commonly employed molecular typing method for M. hyopneumoniae is Multiple-Locus Variable number tandem repeat Analysis (MLVA). The agreement of a shared terminology and classification for the various techniques, specifically MLVA, has not been described, which makes inferences across the literature unsuitable. Therefore, molecular trends for M. hyopneumoniae have been outlined and a common terminology and classification based on Variable Number Tandem Repeats (VNTR) types has been proposed. K E Y W O R D S genomic classification, Mycoplasma hyopneumoniae, terminology, variability, Variable Number Tandem Repeats | 1841 BETLACH ET AL.

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Effect of sow mass vaccination against Mycoplasma hyopneumoniae on the humoral immune response of newborn piglets

Conti

Takeuti²,

Fiúza³

et al. 2022

Trop Anim Health Prod

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Infection dynamics and genetic variability of Mycoplasma hyopneumoniae in self-replacement gilts

Cited by 18 publications

References 21 publications

Comparison of vaccination protocols against Mycoplasma hyopneumoniae during the gilt acclimation period

Comparison of vaccination protocols against Mycoplasma hyopneumoniae during the gilt acclimation period

Mycoplasma hyopneumoniaevariability:Current trends and proposed terminology for genomic classification

Effect of sow mass vaccination against Mycoplasma hyopneumoniae on the humoral immune response of newborn piglets

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