Infection process in resistant and susceptible maize (Zea mays L.) genotypes to Cercospora zeae-maydis (type II)

Lyimo, H.J.F.; Pratt, Richard C.; Mnyuku, R.S.O.W.

doi:10.17221/57/2011-pps

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…We noted that both RIL387 and RIL165 had differential expression of terpenoid biosynthetic genes that provide the building blocks for phytoalexin production. Phytoalexins have been proposed to provide resistance to C. zeina infection [ 93 ], and therefore we investigated the induction of phytoalexin biosynthetic gene expression and phytoalexin accumulation in both genotypes using a glass house time course analysis of maize leaves inoculated with C. zeina . Both families of maize phytoalexins accumulated in response to C. zeina , albeit, zealexins were more abundantly elicited than kauralexins in both genotypes.…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq analysis of resistant and susceptible sub-tropical maize lines reveals a role for kauralexins in resistance to grey leaf spot disease, caused by Cercospora zeina

et al. 2017

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Background Cercospora zeina is a foliar pathogen responsible for maize grey leaf spot in southern Africa that negatively impacts maize production. Plants use a variety of chemical and structural mechanisms to defend themselves against invading pathogens such as C. zeina, including the production of secondary metabolites with antimicrobial properties. In maize, a variety of biotic and abiotic stressors induce the accumulation of the terpenoid phytoalexins, zealexins and kauralexins.Results C. zeina-susceptible line displayed pervasive rectangular grey leaf spot lesions, running parallel with the leaf veins in contrast to C. zeina-resistant line that had restricted disease symptoms. Analysis of the transcriptome of both lines indicated that genes involved in primary and secondary metabolism were up-regualted, and although different pathways were prioritized in each line, production of terpenoid compounds were common to both. Targeted phytoalexin analysis revealed that C. zeina-inoculated leaves accumulated zealexins and kauralexins. The resistant line shows a propensity toward accumulation of the kauralexin B series metabolites in response to infection, which contrasts with the susceptible line that preferentially accumulates the kauralexin A series. Kauralexin accumulation was correlated to expression of the kauralexin biosynthetic gene, ZmAn2 and a candidate biosynthetic gene, ZmKSL2. We report the expression of a putative copalyl diphosphate synthase gene that is induced by C. zeina in the resistant line exclusively.DiscussionThis study shows that zealexins and kauralexins, and expression of their biosynthetic genes, are induced by C. zeina in both resistant and susceptible germplasm adapted to the southern African climate. The data presented here indicates that different forms of kauralexins accumulate in the resistant and susceptible maize lines in response to C. zeina, with the accumulation of kauralexin B compounds in a resistant maize line and kauralexin A compounds accumulating in the susceptible line.Electronic supplementary materialThe online version of this article (10.1186/s12870-017-1137-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

RNA-Seq analysis of resistant and susceptible sub-tropical maize lines reveals a role for kauralexins in resistance to grey leaf spot disease, caused by Cercospora zeina

et al. 2017

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“…In Ethiopia, reported that yield losses due to gray leaf spot on resistant, moderately resistant, and susceptible varieties were between 0-14. 9%, 13.7-18.3% and 20.8-49.5%, respectively in Bako and its surrounding areas (Lyimo et al, 2013;.…”

Section: Introductionmentioning

confidence: 91%

“…Gray leaf spot caused by Cercospora zeae maydis has become one of the major yield-limiting diseases of maize in eastern Africa including Ethiopia ( Bekeko et al, 2018). This disease is most severe and damaging when periods of high relative humidity extended, slowdrying of dews and prolonged late-season fogs (Lyimo et al, 2013). Increased incidence of gray leaf spot in Ethiopia has been associated with cultural practices such as reduced tillage, continuous cultivation of maize, and use of susceptible maize cultivars (Wegary et al, 2008;Worku et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Participatory evaluation of maize varieties for the management of maize gray leaf spot disease (Cercospora zeae maydis) at Gondar Zuria district, northwest Ethiopia

Mengist¹,

Moges²

2019

J. Dryland Agric.

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Gray leaf spot is one of the major constraints of maize production in Ethiopia where warm humid environmental condition is prevailing. A field experiment was conducted in Gondar zuria district of western Ethiopia during 2016/17 and 2017/18 cropping season with the aim to evaluate effective maize varieties for the management of maize gray leaf spot disease. The experiment was laid out in Randomized Complete Block Design (RCBD) in three replications. Nine improved varieties, that is, SBRH, Gibie 2, Gibie 3, Argene, Jibat, BH-546, SPRH, Wonji, AMR-852 with one local maize were tested for GLS resistance. The genotype significantly varied and there were significant differences (P<0.05) in the overall mean of GLS incidence, severity, AUDPC%/day, yield and yield component among varieties. Data were analyzed using SAS version 9.2. The result indicated that the maximum disease incidence and area under disease progress curve (AUDPC), 58.8 and 214.83% per day respectively were recorded from local maize while the minimum incidence and AUDPC value, 22.7 and 150.49% per day, respectively were recorded from Gibie 2 maize variety. The maximum grain yield of 8611.7 kg/ha was recorded from Gibie 2 variety while the minimum grain yield of 4542.3 kg/ha was recorded from local maize. The study suggested that variety of Gibie 2 showed minimum grain yield loss which result to negligible loss of 1.47%, respectively and showed significant reduction of maize GLS incidence, with a corresponding increased grain yield of maize.

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Combining ability and nature of gene action in maize (Zea mays L) inbred lines for resistance to gray leaf spot disease (Cercospora zeae maydis) in Ethiopia

Bekeko

Fininsa

Wegary³

et al. 2018

Crop Protection

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Infection process in resistant and susceptible maize (Zea mays L.) genotypes to Cercospora zeae-maydis (type II)

Cited by 5 publications

References 39 publications

RNA-Seq analysis of resistant and susceptible sub-tropical maize lines reveals a role for kauralexins in resistance to grey leaf spot disease, caused by Cercospora zeina

RNA-Seq analysis of resistant and susceptible sub-tropical maize lines reveals a role for kauralexins in resistance to grey leaf spot disease, caused by Cercospora zeina

Participatory evaluation of maize varieties for the management of maize gray leaf spot disease (Cercospora zeae maydis) at Gondar Zuria district, northwest Ethiopia

Combining ability and nature of gene action in maize (Zea mays L) inbred lines for resistance to gray leaf spot disease (Cercospora zeae maydis) in Ethiopia

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