Infection, propagation, distribution and stability of plant virus in hairy root cultures

In vitro propagation of plant virus using different forms of plant tissue culture and modes of culture operation

“…Neither application of shooty teratomas instead of hairy roots as the propagation host, nor nutrient supplementation using semi-continuous culture operation, was effective in reducing the extent of infectivity loss. It is known that high virus concentrations are maintained in hairy roots with already-established TMV infections after subculturing and further biomass growth (Shadwick and Doran, 2007b), suggesting that viral infectivity may be recovered depending on the culture conditions applied.…”