Infectivity analysis of a blackgram isolate of Mungbean yellow mosaic virus and genetic assortment with MYMIV in selective hosts

Abstract

Yellow mosaic disease in grain legumes in Indian subcontinent is caused by two important virus species viz. Mungbean yellow mosaic virus (MYMV) and Mungbean yellow mosaic India virus (MYMIV), belonging to the genus Begomovirus of the family Geminiviridae. The genomic components of a begomovirus causing yellow mosaic disease in blackgram in southern India were cloned and sequenced. Nucleotide sequence comparison of DNA A component shows the virus isolate to be a variant of Mungbean yellow mosaic virus:-(MYMV-[IN:Vam:05]). However, DNA B component of the present virus isolate has greater similarity (92%) to Mungbean yellow mosaic India virus. Agroinoculations of the viral clones produced typical yellow mosaic symptoms in blackgram and mungbean, severe leaf curl and stunting in French bean, similar to blackgram isolate of MYMIV. Blackgram isolates of both the virus species were only mildly infectious on cowpea, produced atypical leaf curl symptoms and not yellow or golden mosaic. In agroinoculations done by exchanging genomic components, symptom expression was seen only in French bean. In cowpea, blackgram and mungbean there was no visible symptoms though viral DNA could be detected by PCR.

Breeding for Enhancing Legumovirus Resistance in Mungbean: Current Understanding and Future Directions

“…YMD caused by Begomoviruses (family Geminiviridae, genus Begomovirus) has emerged as the major threat. This disease currently is a major hazard to the flourishing production of mungbean in India, Sri Lanka, Pakistan, Bangladesh, Papua New Guinea, Philippines, and Thailand [6][7][8][9]. YMD was first reported in lima bean (Phaseolus lunatus) in 1940 [10] and was subsequently discovered in other plants, such as Dolichos bean (Dolichos lablab) [11,12] and mungbean [13].…”

Gamma rays induced urdbbean [Vigna mungo (L.) Hepper] mutants with YMV resistance, good batter quality and bold seeded type

“…Of these MYMIV and MYMV are most important as these infect large number of legumes in India. MYMIV is more predominant in northern, central and eastern regions of India (Usharani et al 2004) and MYMV in southern region (Karthikeyan et al 2004;Girish and Usha 2005;Haq et al 2011) to which Tamil Nadu state belongs.…”

Surveillance of Mungbean Yellow Mosaic Virus (MYMV) Incidence and Its Vector Population for Development of Yellow Mosaic Disease Forewarning

“…Correlation between whitefly population and disease incidence showed positive non-significant relation with each other in all the locations. The regression analysis revealed that, per cent contribution of weather factors on whitefly population and disease incidence at Kavalur, Raichur (Haq et al, 2011).…”