Infiltrating T-cell abundance combined with EMT-related gene expression as a prognostic factor of colon cancer

Abstract

EMT-related gene expression reportedly exhibits correlation with the anti-tumor immunity of T cells. In the present study, we explored the factors that might affect the efficacy of immunotherapy in colon cancer with treatment. In this regard, RNA-seq and clinical data of 469 colon cancer samples derived from the Cancer Genome Atlas (TCGA) database were used to calculate infiltrating T-cell abundance (ITA), to illustrate a pathway enrichment analysis, and to construct Cox proportional hazards (CPH) regression models. Subsequently, the RNA-seq and clinical data of 177 colon cancer samples derived from the GSE17536 cohort were used to validate the CPH regression models. We found that ITA showed correlation with EMT-related gene expression, and that it was not an independent prognostic factor for colon cancer. However, upon comparison of two groups with the same ITA, higher EMT expression helped predicted a worse prognosis, whereas a higher ITA could help predict a better prognosis upon comparison of two groups with the same EMT. Additionally, seven genes were found to be statistically related to the prognosis of patients with colon cancer. These results suggest that the balance between ITA and EMT-related gene expression is conducive to the prognosis of patients with colon cancer, and TPM1 is necessary to further explore the common target genes of immune checkpoint blockade.

Identification and Validation of an Anoikis-Related Gene Signature to Predict Prognosis in Colorectal Cancer

“…Previous studies showed that CXCL8 is involved in tumor angiogenesis and has been linked to promoting distant metastases in CRC(Karin 2018, Lukaszewicz-Zajac and Mroczko 2021). Our investigations revealed that CXCL-8 and TPM1, similar to classical tumor markers, predicted poor OS in patients with CRC, which is consistent with the results presented in other studies(Huang, Chen et al 2021, Luo, Tai et al 2022). KLF4 is a transcription factor that not only regulates physiological processes, such as cell proliferation, differentiation, and apoptosis, but also participates in the pathogenesis of in ammation and tumorigenesis(He, He et al 2023).…”

Exploration of the typical features of tubulovillous adenoma using in-depth quantitative proteomics analysis

“…EMT-related proteins were regarded as risk factors for CRC in the previous studies. Huang et al found that an EMT-related gene expression signature combining infiltrating T-cell abundance has showed promising prognostic value in colon cancer [ 37 ]. Further, EMT-related gene expression signature can also be applied as a risk stratified tool in hepatocellular cancer [ 38 ].…”