Infinite Coordination Polymer Nanoparticles Used for Fluorescence Turn-On Sensing of Ascorbic Acid

Abstract

In this work, a “turn-on” fluorescence assay with sequential recognition based on infinite coordination polymer (ICP) nanoparticles is developed for the highly selective analysis of ascorbic acid (AA). The ICP nanoparticles are easily prepared by self-assembly of reactive Ce4+, fluorescent Tb3+, and the bridging ligand adenosine monophosphate (AMP), which are denoted as AMP-Ce­(IV)/Tb ICP nanoparticles. The weak fluorescence of the AMP-Ce­(IV)/Tb ICP nanoparticles can be selectively switched on by AA via two sequential recognitions: (1) Ce4+ in the AMP-Ce­(IV)/Tb ICPs is regarded as the first recognition component to react with AA through a redox reaction, and (2) Tb3+ is the second recognition site that will be sensitized by the oxidation product of AA, thus emitting strong fluorescence. The principle of dual recognition endows the proposed assay with a high specificity, which can well eliminate the interference caused by similar reductive biomolecules in the detection of AA. The as-proposed turn-on fluorescence assay possesses a wide linear range of 0 to 100 μM and a low detection limit of 190 nM for AA. Furthermore, the levels of AA in vitamin C tablets and serum samples have been precisely determined. The present strategy provides an efficient way to highly recognize AA with merits of direct sensing, simple operation, superior selectivity, and excellent sensitivity, which also creates more possibilities for the application of ICP nanoparticles.

Recent progress in 0D optical nanoprobes for applications in the sensing of (bio)analytes with the prospect of global health monitoring and detailed mechanistic insights

“…These polymer dots can be easily coupled with small organic molecules or biomolecules to tune the energy transfer process to be applied for target-specific detection in complex biological matrices. Nowadays, Pdots have emerged as flexible probes for the detection of numerous biomolecules such as ascorbic acid, quercetin, glutathione, intracellular pH, and alkaline phosphatase, etc., [259][260][261][262][263][264][265][266][267][268][269] via the chromogenic and/or fluorogenic strategy through tunable IFE, FRET, PET, static and dynamic quenching mechanism (Table 6).…”

“…This method acquired an LOD of 190 nM towards the detection of AA. 259 The sensitivity of the designed nanosensor was investigated in real serum samples and vitamin C samples with qualified recoveries.…”

“…LOD of 190 nM towards the detection of AA. 259 The sensitivity of the designed nanosensor was investigated in real serum samples and vitamin C samples with qualified recoveries. Again, the detection of the natural flavonoid quercetin (Qc) (3,5,7-trihydroxy-2-(3,4-dihydroxyphenyl)-4H-chromen-4-one) is significant due to its important biochemical and pharmacological activities, such as anti-oxidant and anti-inflammatory, etc.…”

Ultrarapid Microwave-Assisted Synthesis of Fluorescent Silver Coordination Polymer Nanoparticles and Its Application in Detecting Alkaline Phosphatase Activity

“…Alkaline phosphatase (ALP) as a zinc-containing dimeric enzyme has the specific ability to remove phosphate groups from a variety of phosphorylated substrates, such as nucleic acids, proteins, and other phosphate-group-containing molecules (such as ascorbic acid 2-phosphate, AA2P) [ 1 , 2 , 3 ]. ALP is widely distributed in various tissues of living organisms.…”

A Cd(ii)-organic framework as a highly sensitive and rapid fluorometric sensor for ascorbic acid in aqueous medium

“…Ascorbic acid (AA), which is also called vitamin C, has various essential roles in human biological systems. 1 AA is required for several metabolic processes of the body like protein and adrenaline preparation and iron absorption. 2 Since the human body can't produce AA, it is taken from the outer sources through different foods and medicines.…”