Inflammation and type 2 diabetes

Calle, Mariana C.; Fernández, María Luz

doi:10.1016/j.diabet.2011.11.006

Cited by 413 publications

(303 citation statements)

References 90 publications

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“…ROS generation and AKT activation are required for D-ASC cytokine production An increased level of proinflammatory cytokines is a common feature in diabetes [40]. Thus, we investigated whether D-ASC de-differentiation status might contribute to this proinflammatory profile.…”

Section: Resultsmentioning

confidence: 99%

A diabetic milieu promotes OCT4 and NANOG production in human visceral-derived adipose stem cells

et al. 2012

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Aims/hypothesis Successful outcomes have been obtained by exploiting adipose-derived stem cells (ASCs) in regenerative medicine. NADPH oxidase (NOX)-generated reactive oxygen species (ROS) are known to control stem cell self-renewal. Several high glucose (HG)-mediated effects depend on NOX-generated ROS. In this study, we investigated whether, and how mechanistically, HG concentrations control ASC fate in patients with diabetes. Methods ASCs from the visceral adipose tissue of nondiabetic (N-ASCs) and diabetic participants (D-ASCs), identified by surface markers, were counted and evaluated for ROS generation and stem cell properties. Their ability to release soluble factors was assessed by BioPlex analysis. To reproduce an invitro diabetic glucose milieu, N-ASCs were cultured in HG (25 mmol/l) or normal glucose (NG) concentration (5 mmol/l), as control. ASC pluripotency was assessed by in vitro study. The p47 phox NOX subunit, AKT and octamer-binding transcription factor 4 (OCT4; also known as POU5F1) were knocked down by small-interfering RNA technology. Stem-cell features were evaluated by sphere cluster formation. Results The ASC number was higher in diabetic patients than in non-diabetic controls. Production of OCT4 and NANOG, stem-cell-specific transcription factors, was upregulated in D-ASCs compared with N-ASCs. Moreover, we found that ROS production and AKT activation drove D-ASC, but not N-ASC, secretion. When N-ASCs were cultured in vitro in the presence of HG, they also expressed OCT4/NANOG and formed spheres. By knock-down of the p47 phox NOX subunit, AKT and OCT4 we demonstrated that NOX-generated ROS and their downstream signals are crucial for HG-mediated ASC de-differentiation and proinflammatory cytokine production. Conclusions/interpretation We herein provide a rationale for exploiting D-ASCs in regenerative medicine and/or exploiting HG preconditioning to increase ASCs ex vivo.

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Section: Resultsmentioning

confidence: 99%

A diabetic milieu promotes OCT4 and NANOG production in human visceral-derived adipose stem cells

et al. 2012

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“…TNF-alpha was proposed to have preferentially paracrine effects and to be a regulator of insulin resistance at the tissue level [3][4][5] . Low-grade inflammation is a common feature in subjects with type 2 diabetes 6 as increased levels of circulating inflammatory markers were found to be significantly more prominent in type 2 diabetes 7 . Increased inflammatory markers in type 2 diabetes are believed to originate, at least partially, from adipose tissue 8,9 .…”

Section: Introductionmentioning

confidence: 99%

Impact of moderate versus mild aerobic exercise training on inflammatory cytokines in obese type 2 diabetic patients: a randomized clinical trial

Gari²,

2014

Afr H. Sci.

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Background: Recently some plasma biomarkers of inflammation have been recognized as important cardiovascular risk factors. There is little information about the effects of aerobic exercise training on these biomarkers and the risk of metabolic complications in obese type 2 diabetes patients. Objective: To compare the impact of moderate versus mild aerobic exercise training on the inflammatory cytokines in obese type 2 diabetic patients. Methods: Fifty obese type 2 diabetic patients of both sexes with body mass index (BMI) varying from 31 to 36 kg/m 2 , non smokers, free from respiratory, kidney, liver, metabolic and neurological disorders, participated in this study. Their age ranged from 40 to 55 years. The subjects were included into two equal groups; the first group (A) received moderate aerobic exercise training. The second group (B) received mild aerobic exercise training, three times / week for 3 months. Results:The mean values of leptin, TNF-alpha, IL2, IL4, IL6, HOMA-IR and HBA1c were significantly decreased in group (A) and group (B). Also, there were significant differences between both groups after treatment. Conclusion: Moderate aerobic exercise training modulates inflammatory cytokines more than mild aerobic exercise training in obese type 2 diabetic patients.

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“…Secreted by monocytes in the CNS, they activate and are responsible for cell injury or inflammatory conditions. 10 The microglial cell amount in rats with T2DM rises significantly compared with the control. This could be responsible for the inflammation that usually occurs in T2DM.…”

Section: Resultsmentioning

confidence: 94%

Influence of CSN1S2 protein from Caprine milk Etawah Breed (EB) on histology of microglial cells in rat (Rattus norvegicus) Type-2 diabetes mellitus (T2DM)

Rika

Fatchiyah

2017

AIP Conference Proceedings

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The improvement of the quality of polluted irrigation water through a phytoremediation process in a hydroponic batch culture system AIP Conference Proceedings 1908, 030003 (2017) Abstract. Type-2 diabetes mellitus (T2DM) is a degenerative disease that causes an imbalance in the metabolism. The aim of this research is to determine the influences of CSN1S2 on the structure of microglial cells in T2DM. Rats (Rattus norvegicus) were divided into eight groups of treatment with looping three times each between treatment groups (CM) Control. The control is given a milk treatment with doses of 375 mg/kg (CM375), 750 mg/kg (CM750), and 1500 mg/kg (CM1500), T2DM (DMK), and T2DM with CSN1S2 375 mg/kg dose (DM375), 750mg/kg (DM750), and 1500 mg/kg (DM1500). The animal model T2DM was induced by a high-fat diet in the form of feed followed by injection of STZ (dose of 25 mg/kg of animal treatment) and treatment of CSN1S2 for 28 days. Brain organs were taken and analysed in histopathology stained by Hematoxylin-eosin (HE) and observed using Olympus BX53. Based on the results, it was concluded that CSN1S2 protein is influential for induction of microglial cell proliferation in animal models of T2DM, as immunity responds to the inflammatory condition in T2DM.

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Inflammation and type 2 diabetes

Cited by 413 publications

References 90 publications

A diabetic milieu promotes OCT4 and NANOG production in human visceral-derived adipose stem cells

A diabetic milieu promotes OCT4 and NANOG production in human visceral-derived adipose stem cells

Impact of moderate versus mild aerobic exercise training on inflammatory cytokines in obese type 2 diabetic patients: a randomized clinical trial

Influence of CSN1S2 protein from Caprine milk Etawah Breed (EB) on histology of microglial cells in rat (Rattus norvegicus) Type-2 diabetes mellitus (T2DM)

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