Inflammation by activated macrophage‐like THP‐1 cells increases human dura mater cell adhesion with alteration of integrin α₂β₁ and matrix metalloproteinase

Abstract

This study was designed to investigate (i) extracellular matrix to specify adhesive substrates to human dura mater cell (hDMC); (ii) the alteration on adhesion‐related molecules in hDMC; and (iii) secreted matrix metalloproteinases (MMPs) linked with extracellular matrix remodeling after exposure to inflammation. The hDMC was cultured from human dura mater tissue, and the studies were performed with hDMC after co‐culturing with macrophage like THP‐1 cells (Mϕ). The adhesion of co‐cultured hDMC through collagen I increased 6.4‐fold and through collagen IV increased 5.0‐fold compared with the adhesion of naïve cells (p < 0.001). Integrin subtype α2β1 expression was increased 6.3‐fold (p < 0.001) and α1 expression was decreased 2.0‐fold (p < 0.001) in the co‐cultured cells compared with the naïve cells. Co‐culturing induced significant increases in MMP‐1 (13.9‐fold, p < 0.01), MMP‐3 (7.6‐fold, p < 0.01), and VEGF (VEGF: 3.8‐fold, p < 0.05) expression and decreases in MMP‐9 (0.1‐fold, p < 0.01) compared with the sum of naïve hDMC and Mϕ values. Increased hDMC adhesion under inflammatory conditions is caused by an increased cellular affinity for collagen I and IV mediated by increased hDMC levels of integrin subtype α2β1 and environmental MMP‐1, ‐3 and decreased MMP‐9. Selective integrin subtype α2β1 inhibition assay showed 37.8% and 35.7% reduction in adhesion of co‐cultured hDMC to collagen I (p < 0.001) and IV (p = 0.057), respectively. The present study provides insight into the pathological conditions related to dura mater adhesion in inflammation. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 9999:1–11, 2019.

A systematic comparison of fibroblasts derived from postmortem human dura mater versus dermal epithelium for neurodegenerative disease modeling

“…Expression differences in cell lines derived from dermis and dura mater may be due to the different tissue types and anatomical locations as has been reported for fibroblasts isolated from different body regions such as the arm or thigh [4,7]. Others have reported a lack or decrease of S100 family protein expression in dura mater-derived cells [5,21,22], similar to what we have shown here. Thus, our data suggest that cells cultured from dura mater may not be fibroblasts but rather an alternative, yet unidentified cell type.…”

“…Cells from dura mater can be isolated, cultured, and utilized for several applications. Previous studies have investigated the use of dura mater-derived cells in grafts [9], drug response [10,12], particle engulfment [18,19], inflammation and healing [5,11], and reprogramming into iPSCs [2,3,22]. Brain biobanks oftentimes collect and store dura mater at the time of autopsy.…”

A new side-effect of sufentanil: increased monocyte-endothelial adhesion

“…However, circulating monocytes are prone to adherence to vascular endothelial cells under pathological conditions, especially when the vascular endothelial cells are inflamed and damaged [ 30 , 31 ]. There are numerous risk factors, such as surgical stimulation, inflammation and vascular injury induced by surgery, long durations in the supine position, and chemotherapies, that could result in monocyte-endothelial adherence in patients with cancers or undergoing surgery [ 32 – 34 ]. However, the effects of analgesics on monocyte-endothelial adherence have not been previously reported.…”

A New Side-effect of Sufentanil: Increased Monocyte-endothelial Adhesion

“…However, circulating monocytes are prone to adherence to vascular endothelial cells under pathological conditions, especially when the vascular endothelial cells are in amed and damaged [23,24]. There are numerous risk factors, such as surgical stimulation, in ammation and vascular injury induced by surgery, long durations in the supine position, and chemotherapies, that could result in monocyte-endothelial adherence in patients with cancers or undergoing surgery [25][26][27]. However, the effects of analgesics on monocyte-endothelial adherence have not been previously reported.…”