Inflammatory and alternatively activated human macrophages attract vessel-associated stem cells, relying on separate HMGB1- and MMP-9-dependent pathways

Lolmède, Karine; Campana, Lara; Vezzoli, Michela; Bosurgi, Lidia; Tonlorenzi, Rossana; Clementi, Emilio; Bianchi, Marco E.; Cossu, Giulio; Manfredi, Angelo A.; Brunelli, Silvia; Rovere‐Querini, Patrizia

doi:10.1189/jlb.0908579

Cited by 197 publications

(185 citation statements)

References 47 publications

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“…M2a expressed CD206 and MHC class II and secreted predominantly CCL-22. M2b expressed MHC class II and secreted predominantly TNF-a, IL-10, and IL-6, whereas M2c were MHC class II negative, expressed CD163 and IL-1RII, and secreted high levels of IL-10, confirming the validity of polarization methods applied in the current study (7,(35)(36)(37).…”

Section: Isolation Of Monocytes and Generation Of Macrophage Subsetssupporting

confidence: 60%

Glycoengineering of Therapeutic Antibodies Enhances Monocyte/Macrophage-Mediated Phagocytosis and Cytotoxicity

Herter

Birk

Klein

et al. 2014

The Journal of Immunology

134

100

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Therapeutic Abs possess several clinically relevant mechanisms of action including perturbation of tumor cell signaling, activation of complement-dependent cytotoxicity, Ab-dependent cellular cytotoxicity (ADCC), Ab-dependent cellular phagocytosis (ADCP), and induction of adaptive immunity. In view of the important role of phagocytic lineage cells in the mechanism of action of therapeutic Abs, we analyzed FcγR receptor-dependent effector functions of monocytes and macrophages triggered by glycoengineered (GE) Abs (having enhanced FcγRIIIa [CD16a] binding affinity) versus their wild-type (WT) counterparts under different experimental conditions. We first defined the precise FcγR repertoire on classical and nonclassical intermediate monocytes—M1 and M2c macrophage populations. We further show that WT and GE Abs display comparable binding and induce similar effector functions (ADCC and ADCP) in the absence of nonspecific, endogenous IgGs. However, in the presence of these IgGs (i.e., in a situation that more closely mimics physiologic conditions), GE Abs display significantly superior binding and promote stronger monocyte and macrophage activity. These data show that in addition to enhancing CD16a-dependent NK cell cytotoxicity, glycoengineering also enhances monocyte and macrophage phagocytic and cytotoxic activities through enhanced binding to CD16a under conditions that more closely resemble the physiologic setting.

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Section: Isolation Of Monocytes and Generation Of Macrophage Subsetssupporting

confidence: 60%

Glycoengineering of Therapeutic Antibodies Enhances Monocyte/Macrophage-Mediated Phagocytosis and Cytotoxicity

Herter

Birk

Klein

et al. 2014

The Journal of Immunology

134

100

View full text Add to dashboard Cite

show abstract

“…Indeed, it has been demonstrated that M2a macrophages are essential for survival during helminth infection [25]. It has also been reported that M2a macrophages express low levels of CD86 [15]. The contrasting CD86 expression levels in these macrophages may be due to different stimulation protocols employed in these studies.…”

Section: Discussionmentioning

confidence: 73%

“…This restricted panel was selected based on literature reports, and the involvement of these molecules in macrophage activation [14][15][16]. The expression of the cell surface molecules was determined by flow cytometry analyses setting the expression levels in unpolarized MDMs as the base line.…”

Section: Distinct Cell Surface Marker Profile Of Polarized Macrophagesmentioning

confidence: 99%

Characterization of In vitro Generated Human Polarized Macrophages

Iqbal¹,

Kumar²

2015

J Clin Cell Immunol

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“…Conversely, muscle stem cells influence macrophages. This is the case for vessel-associated muscle stem cells (also referred to as mesoangioblasts), myogenic precursors that express pericyte markers and cross vessel walls; 69,93,94 they sustain the ability of macrophages to clear apoptotic cells and promote the expression of genes associated with their alternative activation. 95 …”

Section: The Predators: Macrophages Scavenge Muscle Debrismentioning

confidence: 99%