Inflammatory cell mapping of the respiratory tract in fatal asthma

Simões, Sílvia de Magalhães; Santos, Mario Adriano Dos; Oliveira, M. Da Silva; Fontes, E. S.; Fernezlian, Sandra; Garippo, A. L.; Castro, Isaac Fernando Díaz; Castro, F. F. M.; Martins, Mílton de Arruda; Saldiva, Paulo Hilário Nascimento; Mauad, Thaís; Dolhnikoff, Marisa

doi:10.1111/j.1365-2222.2005.02235.x

Cited by 113 publications

(105 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In accordance with these studies, the anti-inflammatory effects of aerobic exercise could be attributed to decreased levels of IL-4, IL-5, IgE and also an increase in the antiinflammatory cytokine IL-10 (40,41,61). Although these studies verified the leukocytes infiltration in the airways, they did not evaluate the remodeling in the pulmonary vessels and parenchyma, and only Pastva et al (40) evaluated the perivascular infiltration by leucocytes (4,12,16,27,30,48,50,52,54,56,58,59,61,66). In the present study, we observed that eosinophilic inflammation in the perivascular and parenchymal compartment was inhibited by aerobic conditioning, similarly to what was observed in the eosinophilic inflammation in the airways (61).…”

Section: Discussionmentioning

confidence: 65%

“…Although large and small airways and distal parenchyma inflammation and remodeling are well-established alterations in asthmatic patients, only few human studies have reported inflammatory and structural changes in pulmonary and bronchial arteries in patients that died of asthma (48,16). Additionally, in asthmatic patients, small airway and lung parenchyma inflammation and remodeling have been related to asthma severity (4,12,58,59).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Aerobic conditioning and allergic pulmonary inflammation in mice. II. Effects on lung vascular and parenchymal inflammation and remodeling

Vieira¹,

Andrade²,

Duarte³

et al. 2008

American Journal of Physiology-Lung Cellular and Molecular Phys

View full text Add to dashboard Cite

In addition, some studies demonstrated that aerobic training decreases chronic allergic inflammation in the airways; however, its effects on the pulmonary vessels and parenchyma have not been previously evaluated. Our objective was to test the hypothesis that aerobic conditioning reduces inflammation and remodeling in pulmonary vessels and parenchyma in a model of chronic allergic lung inflammation. Balb/c mice were sensitized at days 0, 14, 28, and 42 and challenged with ovalbumin (OVA) from day 21 to day 50. Aerobic training started on day 21 and continued until day 50. Pulmonary vessel and parenchyma inflammation and remodeling were evaluated by quantitative analysis of eosinophils and mononuclear cells and by collagen and elastin contents and smooth muscle thickness. Immunohistochemistry was performed to quantify the density of positive cells to interleukin (IL)-2, IL-4, IL-5, interferon-␥, IL-10, monocyte chemotatic protein (MCP)-1, nuclear factor (NF)-B p65, and insulin-like growth factor (IGF)-I. OVA exposure induced pulmonary blood vessels and parenchyma inflammation as well as increased expression of IL-4, IL-5, MCP-1, NF-B p65, and IGF-I by inflammatory cells were reduced by aerobic conditioning. OVA exposure also induced an increase in smooth muscle thickness and elastic and collagen contents in pulmonary vessels, which were reduced by aerobic conditioning. Aerobic conditioning increased the expression of IL-10 in sensitized mice. We conclude that aerobic conditioning decreases pulmonary vascular and parenchymal inflammation and remodeling in this experimental model of chronic allergic lung inflammation in mice.

show abstract

Section: Discussionmentioning

confidence: 65%

mentioning

confidence: 99%

Aerobic conditioning and allergic pulmonary inflammation in mice. II. Effects on lung vascular and parenchymal inflammation and remodeling

Vieira¹,

Andrade²,

Duarte³

et al. 2008

American Journal of Physiology-Lung Cellular and Molecular Phys

View full text Add to dashboard Cite

show abstract

“…Post mortem studies have shown that the outer wall of the small airways was a major site of inflammation (especially with eosinophils) [8]. Inflammation in fatal asthma extended to the adjacent alveoli [9] and to the perivascular areas [10]. Studies have also shown loss of alveolar attachments in fatal asthma [11], which positively correlated with both mast cell and eosinophilic inflammation, suggesting roles for these inflammatory cells in alveolar disruption.…”

Section: Pathology Of Small Airways In Asthma and Copdmentioning

confidence: 99%

The role of small airways in obstructive airway diseases: Figure 1.

Burgel¹

2011

Eur Respir Rev

196

139

View full text Add to dashboard Cite

This review article is a summary of a seminar organised by the European Respiratory Society on ''The role of small airways in obstructive airway diseases'' which was held in October 2010 in Amsterdam, the Netherlands.The aims of the seminar were to identify important questions related to small airways involvement in asthma and chronic obstructive pulmonary disease (COPD), and to discuss future approaches based on current and evolving knowledge. Data obtained by pathological and physiological measurements in small airways and their relevance to clinical manifestations and therapeutics in asthma and COPD were reviewed.It was concluded that our knowledge on the roles of small airways in asthma and COPD is limited. Studies of large numbers of well-characterised subjects using multiple methods (genetic characterisation, cell biology and physiology, imaging) and integration of the data using mathematical models are suggested to be of interest. The availability of these techniques coupled with our ability to better target inhaled molecules to small airways provide a unique opportunity for a reappraisal of the relevance of small airways in chronic airway diseases.

show abstract

“…For the assessment of cell densities, a point-counting method described previously was used [15]. Briefly, at least six fields were randomly and systematically sampled in order to assess cell densities in follicular tissue by using a grid with a known area attached to the eyepiece of the microscope.…”

Section: Assessment Of Lfsmentioning

confidence: 99%

Significant increase of CD57⁺cells in pulmonary lymphoid follicles of COPD patients

Olloquequi¹,

Montés²,

Prats³

et al. 2010

Eur Respir J

View full text Add to dashboard Cite

Although the presence of pulmonary lymphoid follicles (LFs) has been associated with the progression of chronic obstructive pulmonary disease (COPD), there is no information regarding the pattern of vascularisation, expression of addressins or inflammatory cell densities within these structures in COPD.Histological and immunohistochemical techniques were used to assess the prevalence, structure, localisation, vascularisation and cell proliferation/apoptosis of LFs, as well as the follicular density of B-and T-lymphocytes, macrophages, dendritic cells and CD57+ cells, in lung tissue of nine nonsmokers, 18 smokers without COPD, 16 smokers with moderate COPD and 16 patients with very severe COPD. The density of CD57 + cells within LFs of COPD patients was significantly increased compared to that of nonsmokers and smokers without COPD (p,0.05). Moreover, the percentage of LF profiles with cell apoptosis was also significantly higher in COPD patients (p50.03). By contrast, no significant differences among groups were observed in the follicular densities of other inflammatory cells, nor in the distribution of blood and lymphatic vessels within LFs. Since CD57 + cells are important effectors of cytotoxicity and immune regulation, an increase in their follicular density supports the hypothesis of local immune dysfunction in COPD.

show abstract

Inflammatory cell mapping of the respiratory tract in fatal asthma

Cited by 113 publications

References 49 publications

Aerobic conditioning and allergic pulmonary inflammation in mice. II. Effects on lung vascular and parenchymal inflammation and remodeling

Aerobic conditioning and allergic pulmonary inflammation in mice. II. Effects on lung vascular and parenchymal inflammation and remodeling

The role of small airways in obstructive airway diseases: Figure 1.

Significant increase of CD57⁺cells in pulmonary lymphoid follicles of COPD patients

Contact Info

Product

Resources

About

Inflammatory cell mapping of the respiratory tract in fatal asthma

Cited by 113 publications

References 49 publications

Aerobic conditioning and allergic pulmonary inflammation in mice. II. Effects on lung vascular and parenchymal inflammation and remodeling

Aerobic conditioning and allergic pulmonary inflammation in mice. II. Effects on lung vascular and parenchymal inflammation and remodeling

The role of small airways in obstructive airway diseases: Figure 1.

Significant increase of CD57+cells in pulmonary lymphoid follicles of COPD patients

Contact Info

Product

Resources

About

Significant increase of CD57⁺cells in pulmonary lymphoid follicles of COPD patients