Inflammatory response is modulated by lincRNACox2 via the NF‑κB pathway in macrophages infected by Mycobacterium tuberculosis

Abstract

long intergenic non-coding rnas (lincrnas) are long non-coding transcripts from the intergenic regions of annotated protein-coding genes. lincrna cyclooxygenase 2 (cox2) is an early-primary response gene regulated by the nF-κB signaling pathway in macrophages. it was found that lincRNACox2 was significantly increased in patients with the Mycobacterium tuberculosis (M. tuberculosis) H37ra strain infection and macrophages, using reverse transcription-quantitative Pcr (rT-qPcr). eliSa, western blotting and rT-qPcr results indicated that the inflammatory response factors tumor necrosis factor-α, interferon-γ, interleukin-6, cox2 and inducible nitric oxide synthase were significantly increased in H37Ra infected macrophages. In addition, the inflammatory regulating proteins nF-κB and Stat3 were significantly increased in H37ra infected macrophages but decreased in lincrnacox2 knockdown macrophages infected with H37ra. Moreover, the knockdown of lincrnacox2 increased the apoptotic rate of H37ra infected macrophages and facilitated the proliferation of H37ra. collectively, the present results suggested that lincrnacox2 may be required for the activation of nF-κB and Stat3, in order to regulate inflammatory responses involved in resistance to M. tuberculosis infection.

Cyclic di-AMP as endogenous adjuvant enhanced BCG-induced trained immunity and protection against Mycobacterium tuberculosis in mice

“…Since UMLILO is absent in mice, we detected some candidates lncRNAs that mediates expressions of immune response genes. It was found that lncRNA cyclooxygenase-2 (cox-2) (lincRNA-Cox2) was significantly increased in patients and macrophages with M. tuberculosis H37Ra infection ( 45 ). Both BCG and rBCG-DisA just induced a slight increase of lincRNA-Cox2 expression in BM cells from immunized mice ( Figure 3C ).…”

Long Non-coding RNAs in Tuberculosis: From Immunity to Biomarkers

“…For one thing, lncRNAs participate in macrophage-mediated immune-inflammatory responses that an inducible program of inflammatory gene expression is central to anti-microbial defenses. For example, lincRNA-Cox2 mediates the activation/repression of immune genes that may activate NF-κB and STAT3 to regulate inflammatory responses for resistance of Mtb infection ( Figure 2A ; Carpenter et al, 2013 ; Li D. et al, 2020 ). In addition, lncRNA-PACER (also known as lncRNA-Cox2) was induced in Mtb infected macrophages, acting as a positive regulator of its proximal pro-inflammatory gene Ptgs-2 (also known as Cox2).…”

LncRNA: A Potential Target for Host-Directed Therapy of Candida Infection

“…LincRNA-Cox2 is an early NF-ĸB responsive gene triggered by TNF-α stimulation [ 166 ]. The inflammatory response in macrophages infected by Mycobacterium tuberculosis and Listeria monocytogenes was also modulated by lincRNA-Cox2 via the activation of NF-κB [ 167 , 168 ]. Mechanistically, Hu et al found that lincRNA-Cox2 reacted with the subunit of NF-κB [ 169 ].…”