Infliximab Partially Alleviates the Bite Force Reduction in a Mouse Model of Temporomandibular Joint Pain

Kim, Sang-Hyon; Son, Chang-Nam; Lee, Hyo‐Jung; Cho, Ho-Chan; Jung, Sungwon; Hur, Ji An; Baek, Won-Ki; Jung, Hye Ra; Hong, Ji Hee

doi:10.3346/jkms.2015.30.5.552

Cited by 21 publications

(29 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…TMDs are also associated with mastication-related pain. 13,14 When TMDs occur due to tissue injury in the relevant structures, mastication becomes painful, leading to decreased bite forces and the eventual reduction in food intake. 9,34,35 It has been suggested that the measurement of bite force may represent a more clinically relevant feature of TMDs than the assessment of orofacial mechanical allodynia.…”

Section: Discussionmentioning

confidence: 99%

“…9,34,35 It has been suggested that the measurement of bite force may represent a more clinically relevant feature of TMDs than the assessment of orofacial mechanical allodynia. 13,14 Recently, several innovative methods were developed to directly test bite force. 13,14,36 Dolan et al 37 used a dolognawmeter to quantify the gnawing functions of three mouse models of orofacial pain.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have suggested that the direct measurement of bite force may represent a novel approach for the quantification of TMD-associated mastication-related nociceptive behaviours in mouse models. 13,14 In addition, feeding behaviours are associated with mastication and could provide another critical indicator for the study of TMD-associated nociceptive behaviours. 7,15 Altered eating patterns, including changes in food intake, eating duration, and eating frequency, could reflect changes in masticatory function caused by TMDs.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nociceptive behavioural assessments in mouse models of temporomandibular joint disorders

et al. 2020

Int J Oral Sci

View full text Add to dashboard Cite

Orofacial pain or tenderness is a primary symptom associated with temporomandibular joint (TMJ) disorders (TMDs). To understand the pathological mechanisms underlying TMDs, several mouse models have been developed, including mechanical stimulus-induced TMD and genetic mouse models. However, a lack of feasible approaches for assessing TMD-related nociceptive behaviours in the orofacial region of mice has hindered the in-depth study of TMD-associated mechanisms. This study aimed to explore modifications of three existing methods to analyse nociceptive behaviours using two TMD mouse models: (1) mechanical allodynia was tested using von Frey filaments in the mouse TMJ region by placing mice in specially designed chambers; (2) bite force was measured using the Economical Load and Force (ELF) system; and (3) spontaneous feeding behaviour tests, including eating duration and frequency, were analysed using the Laboratory Animal Behaviour Observation Registration and Analysis System (LABORAS). We successfully assessed changes in nociceptive behaviours in two TMD mouse models, a unilateral anterior crossbite (UAC)-induced TMD mouse model and a β-catenin conditional activation mouse model. We found that the UAC model and β-catenin conditional activation mouse model were significantly associated with signs of increased mechanical allodynia, lower bite force, and decreased spontaneous feeding behaviour, indicating manifestations of TMD. These behavioural changes were consistent with the cartilage degradation phenotype observed in these mouse models. Our studies have shown reliable methods to analyse nociceptive behaviours in mice and may indicate that these methods are valid to assess signs of TMD in mice.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nociceptive behavioural assessments in mouse models of temporomandibular joint disorders

et al. 2020

Int J Oral Sci

View full text Add to dashboard Cite

show abstract

“…Infliximab is a chimeric immunoglobulin‐G1 monoclonal antibody that targets TNF‐α, most commonly used in immunosuppressive treatments and reportedly contributes to inflammation symptoms . Therefore, the objective of this study was to evaluate the role of TNF‐α in orofacial discomfort in an occlusal dental interference model in rats.…”

Section: Introductionmentioning

confidence: 99%

Tumor necrosis factor alpha mediates orofacial discomfort in an occlusal dental interference model in rats: The role of trigeminal ganglion inflammation

Silva

Martins

Neto

et al. 2019

J Oral Pathology Medicine

View full text Add to dashboard Cite

Background: Tumor necrosis factor alpha (TNF-α) is a proinflammatory cytokine that plays an important role in the early stages of inflammation. In this study, we investigated its role in orofacial discomfort in rats subjected to occlusal dental interference (ODI). Methods:Female Wistar rats (180-200 g) were divided in three groups (n = 30/group):sham group, without ODI, and two experimental groups with ODI pre-treated with 0.1 mL/kg saline (ODI + SAL) or 5 mg/kg infliximab (ODI + INF) and treated every 3 days. The animals were euthanized after 1, 3, and 7 days. The number of bites and scratches and grimace scale scores were determined daily, and the bilateral trigeminal ganglion was histomorphometrically (neuronal body area) analyzed and submitted for immunohistochemistry for TNF-α, nitric oxide synthesis (NOS) neuronal (nNOS) and inducible (iNOS), peroxisome proliferator-activated receptors (PPAR) y (PPARy) and δ/β (PPARδ/β), and glial fibrillary acidic protein (GFAP). One-way/two-way ANOVA/ Bonferroni tests were used (P < .05, GraphPad Prism 5.0). Results:ODI + SAL showed a large number of bites (P = .002), scratches (P = .002), and grimace scores (P < .001) in the firsts days, and ODI + INF partially reduced these parameters. The contralateral and ipsilateral neuronal body area was significantly reduced on day 1 in ODI + SAL, but returned to the basal size on days 3 and 7, by increase in TNF-α, nNOS, PPARy, PPARδ/β, and GFAP immunostaining. The infliximab treatment attenuated these alterations (P < .05). There was no iNOS immunostaining. Conclusion:Occlusal dental interference induced transitory orofacial discomfort by trigeminal inflammatory mediator overexpression, and TNF-α blockage attenuated these processes.

show abstract

“…Finally, it should be noted that despite the high "incidence" (78.3%) of mini-implant penetration in the infrazygomatic crest, there was an overall success rate (96.7%) higher than in previous studies with miniimplants installed in different sites. 5 Jia et al 1 well known that clinical success depends on initial mechanical stability and proper loading. 6 It was highlighted that no patient complained of clinical symptoms; there was no detected mobility, and all patients had stable anchoring performance until the end.…”

mentioning

confidence: 99%

Author's response

Fukaya¹,

Kanzaki²,

Miyamoto³

et al. 2018

American Journal of Orthodontics and Dentofacial Orthopedics

View full text Add to dashboard Cite

Letters to the editor* Unilateral IGF-1 injection for mandibular asymmetry I n an excellent experimental research article in the December 2017 issue, an alternate treatment approach for mandibular asymmetry was discussed in a comprehensive way (Fukaya S, Kanzaki H, Miyamoto Y, Yamaguchi Y, Nakamura Y. Possible alternative treatment for mandibular asymmetry by local unilateral IGF-1 injection into the mandibular condylar cavity: Experimental study in mice. Am J Orthod Dentofacial Orthop 2017;152:820-9). We would, however, like to bring attention to 1 important aspect of the study: the dose of IGF-1 was kept at 20 ml, whereas in a previous study by Suzuki et al, 1 a higher dose of 50 ml was used. Was a dosage calculation method for IGF-1 used in the study? Moreover, a more detailed discussion about the number of mice used for preparation of serial undecalcified frozen sections that in turn were used for histomorphometery, immunohistochemistry, and RT-PCR analysis would be informative and highly appreciated.

show abstract

Infliximab Partially Alleviates the Bite Force Reduction in a Mouse Model of Temporomandibular Joint Pain

Cited by 21 publications

References 26 publications

Nociceptive behavioural assessments in mouse models of temporomandibular joint disorders

Nociceptive behavioural assessments in mouse models of temporomandibular joint disorders

Tumor necrosis factor alpha mediates orofacial discomfort in an occlusal dental interference model in rats: The role of trigeminal ganglion inflammation

Author's response

Contact Info

Product

Resources

About