Inflorescence Developmental Stage-Specific High Temperature Effect on Petal Pigmentation in Chrysanthemum

Puangkrit, Theeraniti; Narumi-Kawasaki, T.; Takamura, Tsutomu; Fukai, Seiichi

doi:10.2525/ecb.56.99

Cited by 6 publications

(3 citation statements)

References 28 publications

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“…The expressions of CHS , CHI , F3H , F3′H , DFR , and ANS were shown to be affected by growth temperature using qRT-PCR in C. morifolium ‘Pelican’ 45 . They fixed the temperature at 20 °C or 30 °C all the day and used ray floret samples at the petal extension stage, early vertical stage, and petal fully open stage; the former two stages were almost consistent with our stages 3 and 4.…”

Section: Discussionmentioning

confidence: 99%

Combination of long-read and short-read sequencing provides comprehensive transcriptome and new insight for Chrysanthemum morifolium ray-floret colorization

Kishi-Kaboshi

Tanaka

Sasaki

et al. 2022

Sci Rep

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Chrysanthemum morifolium is one of the most popular ornamental plants globally. Owing to its large and complex genome (around 10 Gb, segmental hexaploid), it has been difficult to obtain comprehensive transcriptome, which will promote to perform new breeding technique, such as genome editing, in C. morifolium. In this study, we used single-molecule real-time (SMRT) sequencing and RNA-seq technologies, combined them with an error-correcting process, and obtained high-coverage ray-floret transcriptome. The SMRT-seq data increased the ratio of long mRNAs containing complete open-reading frames, and the combined dataset provided a more complete transcriptomic data than those produced from either SMRT-seq or RNA-seq-derived transcripts. We finally obtained ‘Sei Arabella’ transcripts containing 928,645 non-redundant mRNA, which showed 96.6% Benchmarking Universal Single-Copy Orthologs (BUSCO) score. We also validated the reliability of the dataset by analyzing a mapping rate, annotation and transcript expression. Using the dataset, we searched anthocyanin biosynthesis gene orthologs and performed a qRT-PCR experiment to assess the usability of the dataset. The assessment of the dataset and the following analysis indicated that our dataset is reliable and useful for molecular biology. The combination of sequencing methods provided genetic information and a way to analyze the complicated C. morifolium transcriptome.

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Section: Discussionmentioning

confidence: 99%

Combination of long-read and short-read sequencing provides comprehensive transcriptome and new insight for Chrysanthemum morifolium ray-floret colorization

Kishi-Kaboshi

Tanaka

Sasaki

et al. 2022

Sci Rep

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“…The flower color was one of the most important quality aspects in ornamental plants (Puangkrit et al, 2018). Flower color depends on the accumulation of secondary compounds, including flavonoids, carotenoids, or betalains.…”

Section: Color Of Chrysanthemum Flowersmentioning

confidence: 99%

Effect of the Different Soil Types on Chrysanthemum Cut Flower Quality

Shintiavira¹,

Sulistyaningsih

Purwantoro

et al. 2021

Caraka Tani J. Sustain. Agric.

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Cultivating annual crops in the high upland has the potential to increase avalanche and land erosion. Therefore, chrysanthemum extensification from high upland to medium upland can create sustainable agriculture. The differences between high upland and medium upland are microclimate and soil type. Meanwhile, the effect of soil type between the high upland and the medium upland on the chrysanthemum spray type quality is unknown. The study aimed to determine the effect of different soil types on the quality of chrysanthemums when planted in high upland. The study used a split plot design with two factors. The first factor was the type of soil, such as Andosol from the high upland and Latosol from the medium upland. The second factor was the chrysanthemum varieties, such as Yastayuki (white flower), Arosuka Pelangi (yellow flower) and Socakawani (red flower). The results showed that both Andosol and Latosol soil types could produce the same quality of chrysanthemum in all varieties when planted in high upland. The quality aspects of chrysanthemum were the plant height, the stem diameter, the number of flowers and flower color. Consequently, we could extend the cultivation of chrysanthemum in medium upland by using Latosol type soil.

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“…Studies by Nozaki et al and Huh et al reported that the pale pigmentation in pink-flowered genotypes of chrysanthemum was mainly due to a reduction of two anthocyanins (Cy 3-6’’-MMG and Cy 3-3’’,6’’-DMG) under high temperature [ 91 , 92 ]. The total anthocyanin content was found to be considerably higher at 20 °C compared with 30 °C in chrysanthemum petals [ 93 ]. Expression of anthocyanin biosynthesis-related genes, including CHS1 , CHS2 , CHI , F3H2 , C3′H , DFR1 , DFR2 , and ANS was downregulated in ray petals when the plants were exposed to 30 °C.…”

Section: External Factors Regulating Anthocyanin Biosynthesis and mentioning

confidence: 99%

Anthocyanins in Floral Colors: Biosynthesis and Regulation in Chrysanthemum Flowers

Mekapogu

Vasamsetti

Kwon

et al. 2020

IJMS

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Chrysanthemum (Chrysanthemum morifolium) is an economically important ornamental crop across the globe. As floral color is the major factor determining customer selection, manipulation of floral color has been a major objective for breeders. Anthocyanins are one of the main pigments contributing to a broad variety of colors in the ray florets of chrysanthemum. Manipulating petal pigments has resulted in the development of a vast range of floral colors. Although the candidate genes involved in anthocyanin biosynthesis have been well studied, the genetic and transcriptional control of floral color remains unclear. Despite advances in multi-omics technology, these methods remain in their infancy in chrysanthemum, owing to its large complex genome and hexaploidy. Hence, there is a need to further elucidate and better understand the genetic and molecular regulatory mechanisms in chrysanthemum, which can provide a basis for future advances in breeding for novel and diverse floral colors in this commercially beneficial crop. Therefore, this review describes the significance of anthocyanins in chrysanthemum flowers, and the mechanism of anthocyanin biosynthesis under genetic and environmental factors, providing insight into the development of novel colored ray florets. Genetic and molecular regulatory mechanisms that control anthocyanin biosynthesis and the various breeding efforts to modify floral color in chrysanthemum are detailed.

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Inflorescence Developmental Stage-Specific High Temperature Effect on Petal Pigmentation in Chrysanthemum

Cited by 6 publications

References 28 publications

Combination of long-read and short-read sequencing provides comprehensive transcriptome and new insight for Chrysanthemum morifolium ray-floret colorization

Combination of long-read and short-read sequencing provides comprehensive transcriptome and new insight for Chrysanthemum morifolium ray-floret colorization

Effect of the Different Soil Types on Chrysanthemum Cut Flower Quality

Anthocyanins in Floral Colors: Biosynthesis and Regulation in Chrysanthemum Flowers

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