Influence de l'�ge de la culture sur la survie de Rhizobium meliloti � la lyophilisation et � la conservation apr�s lyophilisation

Amarger, Noëlle; Jacquemetton, Michèle; Blond, G.

doi:10.1007/bf00412642

Cited by 4 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dye (1982) reported that growth stage had no effect on survival of rhizobia immediately after desiccation at different R.H. levels. In contrast, young cells (24 h) of Sinorhizobium meliloti (formerly Rhizobium meliloti ) are more tolerant of freeze‐drying than older (96 h) cultures ( Amarger et al . 1972 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of growth phases and desiccation on the degrees of unsaturation of fatty acids and the survival rates of rhizobia

Boumahdi¹,

Mary

Hornez³

1999

Journal of Applied Microbiology

View full text Add to dashboard Cite

M . B OU M AH DI , P. MA R Y A ND J .-P. H OR NE Z . 1999. The influence of growth phase on the evolution of cellular fatty acids (CFA) and survival of Sinorhizobium and Bradyrhizobium during desiccation and storage at different levels of relative humidity (R.H.) was studied. Lactobacillic, cis vaccenic and palmitic acids were the major fatty acids of S. meliloti RCR 2011, B. elkanii USDA 120 and B. japonicum 3.2, whatever the growth phase. An exchange of cis vaccenic with lactobacillic acid was observed during the course of growth. The degree of unsaturation (% unsaturated CFA/% saturated CFA u/s ratio) was significantly higher during the mid logarithmic phase of growth. Survival rates immediately after desiccation were unaffected by the growth phase and the R.H. Furthermore, no correlation was found between survival rate and u/s ratio. During the course of desiccation, the u/s ratio of rhizobia decreased but the decrease was largely independent of the R.H. Optimum R.H. values for storage were in the range 22-67·8%, and S. meliloti was significantly more tolerant than the bradyrhizobia. Cells of rhizobia harvested in the lag phase of growth were more resistant to protracted storage than cells at other growth phases. Again, no correlation was found between u/s ratio and survival rates, despite the expected practical significance for screening for drought-tolerant micro-organisms.

show abstract

Section: Discussionmentioning

confidence: 99%

“…1995 ). However, the effect of growth stage on the tolerance of bacteria to desiccation stresses and storage in the dried state is unclear as both no effect ( Dye 1982; Caesar & Burr 1991; Cliquet & Catroux 1994), and a greater tolerance of stationary phase cells ( Amarger et al . 1972 ; Mary et al .…”

Section: Introductionmentioning

confidence: 99%