Influence of Allyl Isothiocyanate on the Soil Microbial Community Structure and Composition during Pepper Cultivation

Gao, Junyuan; Pei, Hongxia; Xie, Hua

doi:10.4014/jmb.2012.12016

Cited by 12 publications

(8 citation statements)

References 67 publications

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“…In Gao's study, A‐ITC reduced the diversity of soil bacteria, stimulated the diversity of the soil fungal community, and significantly changed the structure of fungal community. A‐ITC decreased the relative abundance of dominant bacteria Planctomycetes , Acinetobacter , Pseudodeganella , and RB41, but increased that of Lysobacter , Sphingomonas , Pseudomonas , Luteimonas , Pseudoxanthomonas , and Bacillus at the genera level, while for fungi, Trichoderma , Neurospora , and Lasiodiplodia decreased significantly and Aspergillus , Cladosporium , Fusarium , Penicillium , and Saccharomyces were higher than the control 35 …”

Section: Discussionmentioning

confidence: 85%

“…A-ITC decreased the relative abundance of dominant bacteria Planctomycetes, Acinetobacter, Pseudodeganella, and RB41, but increased that of Lysobacter, Sphingomonas, Pseudomonas, Luteimonas, Pseudoxanthomonas, and Bacillus at the genera level, while for fungi, Trichoderma, Neurospora, and Lasiodiplodia decreased significantly and Aspergillus, Cladosporium, Fusarium, Penicillium, and Saccharomyces were higher than the control. 35 In addition, isothiocyanate hydrolase promoted fungal growth in the presence of ITCs, and it contributed to the virulence of S. sclerotiorum on glucosinolate-producing plants. 3 In Pectobacteria and Pseudomonas syringae, SaxA was the enzyme responsible for the breakdown of 2PE-ITC, and therefore contributing to their virulence on Brassica plants.…”

Section: Discussionmentioning

confidence: 99%

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Antifungal mechanism of isothiocyanates against Cochliobolus heterostrophus

Jia

Zhao

et al. 2022

Pest Management Science

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Background Isothiocyanates (ITCs) generated from the ‘glucosinolates‐myrosinase’ defense system in the Brassicaceae exhibit broad antagonistic activity to various fungal pathogens. Nevertheless, the antifungal activity of ITCs to non‐adapted fungi of Brassicaceae plants were seldom determined. The inhibitory effects of ITCs on Cochliobolus heterostrophus were evaluated and the antagonistic mechanism was explored. Results The mycelium growth of C. heterostrophus was hindered significantly by allyl, 4‐(methylthio)‐butyl, and phenyethyl ITCs, 4MTB‐ITC exhibited the highest inhibitory effect on mycelium growth with an IC50 value of 53.4 μmol L−1. In addition, ITCs exhibited obvious inhibitory effect on conidia germination and pathogenicity of C. heterostrophus. Proteomic analysis indicated that the inhibition of C. heterostrophus by A‐ITC downregulated the expression of genes related to energy metabolism, oxidoreductase activity, melanin biosynthesis, and cell wall‐degrading enzymes. Furthermore, mutants ΔChtrx2 and ΔChnox1 showed increased sensitivity to ITCs, and melanin biosynthesis was inhibited significantly in C. heterostrophus in response to A‐ITC. Interestingly, unlike other pathogens that infected Brassicaceae plants, the SaxA in C. heterostrophus displayed no function in ITC degradation. In addition, the ITCs also exhibited obvious inhibitory effect on mycelium growth of Setosphaeria turcica, Fusarium graminearum, and Magnaporthe oryzae. Conclusion This study indicated that non‐Brassicaceae‐adapted pathogens are more sensitive to ITCs, and ITCs could have applications in protecting non‐Brassicaceae crops in future. In addition, loss of ChNOX1 and ChTRX2 increased the sensitivity of C. heterostrophus to ITCs. Our results provided potential utilization of ITCs to control diseases caused by non‐Brassicaceae pathogenic fungi. © 2022 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Antifungal mechanism of isothiocyanates against Cochliobolus heterostrophus

Jia

Zhao

et al. 2022

Pest Management Science

View full text Add to dashboard Cite

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“…Previous studies have reported soil bacterial community dynamics in relation to the death rate of P. notoginseng in a continuous cropping system [36]. AITC decreased the relative abundances of dominant bacteria, Planctomycetes, Acinetobacter, Pseudodeganella, and RB41, but increased those of Lysobacter, Sphingomonas, Pseudomonas, Luteimonas, Pseudoxanthomonas, and Bacillus, at the genus level in pepper [37]. However, in tomato, there were signi cant increases in the relative abundances of probiotics, such as Sphingomonas and Streptomyces, following AITC fumigation [31].…”

Section: Discussionmentioning

confidence: 99%

Effects of allyl isothiocyanate fumigation on medicinal plant root knot disease control, plant survival, and the soil bacterial community

Yan

et al. 2023

Preprint

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Background Allyl isothiocyanate (AITC) is a natural product with high volatility that is used as a biofumigant to alleviate soil-borne plant diseases, and problems such as root knot nematodes (RKNs) that necessitate continuous cropping. However, little research has assessed the effects of AITC fumigation on medicinal plant. Results The current results indicated that AITC significantly reduced the population of RKNs in soil (p < 0.0001) and showed an excellent RKN disease control effect within 6 months after sowing Panax notoginseng (p < 0.0001). The seedling survival rate of 2-year-old P. notoginseng was approximately 1.7-fold higher after soil treatment with AITC (p = 0.1008). 16S rRNA sequencing indicated that the AITC treatment affected bacterial richness rather than diversity in the CC soil. Furthermore, biomarkers with statistical differences between AITC-treated and untreated CC soil showed that Pirellulales (o), Pirellulaceae (f), Pseudomonadaceae (f), and Pseudomonas (g) played important roles in the AITC-treated group. In addition, the microbiome functional phenotypes predicted using the BugBase tool suggested that AITC treatment is more conducive to improving CC soil through changes in the bacterial community structure. Crucially, our research confirmed that AITC soil treatment significantly increases soil organic matter (p = 0.0055), total nitrogen (p = 0.0054), and available potassium (p = 0.0373), which promotes the succeeding medicinal plant (Polygonatum kingianum) survival. Conclusion AITC as an ecologically friendly soil treatment method, which does not affect soil microbial diversity, but rather relative abundance. Also, it could be providing a basis for a useful agricultural soil management measure to alleviate soil sickness.

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“…In pepper soil, AITC soil fumigation decreased the richness of Planctomycetes , Acinetobacter , Pseudodeganella , and RB41, but increased those of Lysobacter , Sphingomonas , Pseudomonas , Luteimonas , Pseudoxanthomonas , and Bacillus , at the genus level [ 48 ]. In tomato soil, there were significant increases in the richness of probiotics, such as Sphingomonas and Streptomyces , following AITC fumigation [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of allyl isothiocyanate fumigation on medicinal plant root knot disease control, plant survival, and the soil bacterial community

Li,

Lu,

Xia

et al. 2023

BMC Microbiol

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Background Allyl isothiocyanate (AITC) is a natural product with high volatility that is used as a biofumigant to alleviate soil-borne plant diseases, and problems such as root knot nematodes (RKNs) that necessitate continuous cropping. However, little research has assessed the effects of AITC fumigation on medicinal plants. Results AITC significantly reduced the population of RKNs in soil (p < 0.0001) and showed an excellent RKN disease control effect within 6 months after sowing Panax notoginseng (p < 0.0001). The seedling survival rate of 2-year-old P. notoginseng was approximately 1.7-fold higher after soil treatment with AITC (p = 0.1008). 16S rRNA sequencing indicated that the AITC treatment affected bacterial richness rather than diversity in consecutively cultivated (CC) soil. Furthermore, biomarkers with statistical differences between AITC-treated and untreated CC soil showed that Pirellulales (order), Pirellulaceae (family), Pseudomonadaceae (family), and Pseudomonas (genus) played important roles in the AITC-treated group. In addition, the microbiome functional phenotypes predicted using the BugBase tool suggested that AITC treatment is more conducive to improving CC soil through changes in the bacterial community structure. Crucially, our research also suggested that AITC soil treatment significantly increases soil organic matter (p = 0.0055), total nitrogen (p = 0.0054), and available potassium (p = 0.0373), which promotes the survival of a succeeding medicinal plant (Polygonatum kingianum). Conclusion AITC is an ecologically friendly soil treatment that affects the top 10 bacterial richness but not diversity. It could also provide a basis for a useful agricultural soil management measure to alleviate soil sickness.

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Influence of Allyl Isothiocyanate on the Soil Microbial Community Structure and Composition during Pepper Cultivation

Cited by 12 publications

References 67 publications

Antifungal mechanism of isothiocyanates against Cochliobolus heterostrophus

Antifungal mechanism of isothiocyanates against Cochliobolus heterostrophus

Effects of allyl isothiocyanate fumigation on medicinal plant root knot disease control, plant survival, and the soil bacterial community

Effects of allyl isothiocyanate fumigation on medicinal plant root knot disease control, plant survival, and the soil bacterial community

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