Influence of bioactive linker molecules on protein adsorption

Killian, Manuela S.; Schmuki, Patrik

doi:10.1002/sia.5497

Cited by 13 publications

(11 citation statements)

References 56 publications

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“…Fragments of PO 3 – ( m / z = 78.96), PO 2 – ( m / z = 62.96), TiO 2 PO 3 – ( m / z = 158.82), and TiOPO 3 – ( m / z = 142.89) are present in the metal oxide surface, confirming that the priming technique was successful. Combination fragments in ToF-SIMS were shown to only appear if the materials are bound or at least in close contact to each other . The TiO 2 PO 3 – ( m / z = 158.82) and TiOPO 3 – ( m / z = 142.89) fragments demonstrate that the metal oxide reacts with the phosphoric acid solution.…”

Section: Resultsmentioning

confidence: 99%

Metal–Phosphate Bilayers for Anatase Surface Modification

Monteiro

Cha

Schmuki

et al. 2018

ACS Appl. Mater. Interfaces

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Compared to many other metal oxides, anatase TiO shows relatively lower reactivity toward carboxylic acid anchor groups. The latter is crucial for applications, for example, in dye-sensitized solar cells (DSSCs), where the most used dyes bind to the metal oxide surface through carboxylic acid terminations. To improve the surface reactivity, metal-phosphate bilayers of Ni or Co were synthesized on anatase TiO compact oxide and nanotubes. In both cases, time-of-flight secondary ion mass spectrometry (ToF-SIMS) and X-ray photoelectron spectroscopy (XPS) results showed that the bilayers were successfully formed and that the phosphate layer works as an intermediate between TiO and the other species. ToF-SIMS depth profiles of modified nanotubes showed that Ni and Co are present through the whole tube length and reduce in content after heat treatment, in agreement with XPS results. Phosphate groups, on the other hand, are more present in the tubes' depth, and their content on the surface is reduced upon exposure to temperature. The reactivity of the modified surfaces toward carboxylic acid-terminated molecules, as stearic acid and Ru-based N719 dye, was evaluated. Contact angle measurements together with dye desorption experiments demonstrated that the Co-phosphate bilayers heat-treated at 300 °C resulted in the largest enhancement compared to the reference. Bilayer-modified compact anatase TiO and anatase TiO nanotubes were utilized as photoanodes in DSSCs. An increase in efficiency was observed for all modified electrodes with phosphate-Co treatment, leading to the highest J values and an efficiency improvement of 48%.

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Section: Resultsmentioning

confidence: 99%

Metal–Phosphate Bilayers for Anatase Surface Modification

Monteiro

Cha

Schmuki

et al. 2018

ACS Appl. Mater. Interfaces

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Section: Polymer and Biomolecule Conjugationmentioning

confidence: 99%

“…A self-assembled functional monolayer on the surface of TiO 2 NSs can lead to a selective conjugation of biomolecules, including proteins, ligands and antibodies, as well as the adhesion of mammalian cells. The formation of self-assembled monolayers on the surface of TiO 2 NSs is more or less limited to carbonyldiimidazole, phosphonic acid, and organosilane-based reactive components [ 96 ]. Salonen and co-workers have also introduced a functional combination of carbon into the lattice and onto their surface to improve bioactivities [ 97 , 98 ].…”

Section: Post-fabrication Of Tio 2 Nssmentioning

confidence: 99%

“…In addition, the conjugation of biomolecules such as proteins, enzymes, and antibodies on the surface of nanomaterials plays a critical role in facilitating the detection of specific cells and therapeutic delivery to intracellular compartments, while reducing the risk of macrophages [ 104 ]. The biomolecules can be conjugated on the surface of TiO 2 NSs through various functional chemical linkers that can provide a rapid conjugation strategy to limit any bioactivity losses [ 96 , 102 ]. Polymers, antibodies, and therapeutics can be conjugated through one of the following strategies (see Bioconjugate Techniques [ 105 ]): Carbodiimide chemistry A specific and practical conjugation strategy binds the primary amines of biomolecules and polymers to the surface of carboxyl-reactive TiO 2 NSs by means of the water-soluble 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC for an aqueous synthesis) and water-insoluble dicyclohexyl carbodiimide (DCC for an organic reaction).…”

Section: Post-fabrication Of Tio 2 Nssmentioning

confidence: 99%

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Insights into Theranostic Properties of Titanium Dioxide for Nanomedicine

Kafshgari

Goldmann

2020

Nano-Micro Lett.

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HIGHLIGHTS • Multifunctional TiO 2 nanostructures hold promise for advancing a wide range of biomedical applications due to a feasible integration of distinct theranostic features. • Fabrication and post-fabrication strategies implemented to generate multifunctional TiO 2 nanostructures for a broad range of biomedical applications are briefly outlined. The opportunities and challenges of TiO 2 nanomaterials are highlighted in order to open the possibility of clinical translation. ABSTRACT Titanium dioxide (TiO 2 ) nanostructures exhibit a broad range of theranostic properties that make them attractive for biomedical applications. TiO 2 nanostructures promise to improve current theranostic strategies by leveraging the enhanced quantum confinement, thermal conversion, specific surface area, and surface activity. This review highlights certain important aspects of fabrication strategies, which are employed to generate multifunctional TiO 2 nanostructures, while outlining post-fabrication techniques with anemphasis on their suitability for nanomedicine. The biodistribution, toxicity, biocompatibility, cellular adhesion, and endocytosis of these nanostructures, when exposed to biological microenvironments, are examined in regard to their geometry, size, and surface chemistry. The final section focuses on recent biomedical applications of TiO 2 nanostructures, specifically evaluating therapeutic delivery, photodynamic and sonodynamic therapy, bioimaging, biosensing, tissue regeneration, as well as chronic wound healing.

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“…The protein was immobilized via the linker molecule 1,1 0 -carbonyldiimidazole (CDI), as this linker provides a high protein coverage of TiO 2 . 26 Surface analytical techniques based on mass spectrometry can provide insights into the protein structure at interfaces. Whereas techniques like hydrogen-deuterium exchange mass spectrometry provide information about protein conformation upon interactions with, e.g., ligands, 27 time-of-flight secondary ion mass spectrometry (ToF-SIMS) was shown to be extremely valuable for the determination of the orientation of proteins adsorbed to solid supports.…”

Section: Introductionmentioning

confidence: 99%

Stabilization of dry protein coatings with compatible solutes

2018

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Exposure of protein modified surfaces to air may be necessary in several applications. For example, air contact may be inevitable during the implantation of biomedical devices, for analysis of protein modified surfaces, or for sensor applications. Protein coatings are very sensitive to dehydration and can undergo significant and irreversible alterations of their conformations upon exposure to air. With the use of two compatible solutes from extremophilic bacteria, ectoine and hydroxyectoine, the authors were able to preserve the activity of dried protein monolayers for up to >24 h. The protective effect can be explained by the preferred exclusion model; i.e., the solutes trap a thin water layer around the protein, retaining an aqueous environment and preventing unfolding of the protein. Horseradish peroxidase (HRP) immobilized on compact TiO was used as a model system. Structural differences between the compatible solute stabilized and unstabilized protein films, and between different solutes, were analyzed by static time-of-flight secondary ion mass spectrometry (ToF-SIMS). The biological activity difference observed in a colorimetric activity assay was correlated to changes in protein conformation by application of principal component analysis to the static ToF-SIMS data. Additionally, rehydration of the denatured HRP was observed in ToF-SIMS with an exposure of denatured protein coatings to ectoine and hydroxyectoine solutions.

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Influence of bioactive linker molecules on protein adsorption

Cited by 13 publications

References 56 publications

Metal–Phosphate Bilayers for Anatase Surface Modification

Metal–Phosphate Bilayers for Anatase Surface Modification

Insights into Theranostic Properties of Titanium Dioxide for Nanomedicine

Stabilization of dry protein coatings with compatible solutes

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