Influence of cell adhesive molecules attached onto PEG-lipid-modified fluid surfaces on cell adhesion

RGD peptide in cancer targeting: Benefits, challenges, solutions, and possible integrin–RGD interactions

“…Therefore, the introduction of the SGS sequence into c(RGDyK)‐liposomes serves as a strategy to mitigate the immunogenicity‐associated issue and enhance the safety profile of these drug delivery systems. Incorporating RGD peptides into stealth lipids, such as polyethylene glycol (PEG)‐lipids, can help reduce immunogenicity by shielding the peptide from the immune system 88–90 . This approach can also prolong the circulation time of the RGD peptide in the bloodstream, allowing for more effective delivery to the target site.…”

“…Incorporating RGD peptides into stealth lipids, such as polyethylene glycol (PEG)‐lipids, can help reduce immunogenicity by shielding the peptide from the immune system. 88 , 89 , 90 This approach can also prolong the circulation time of the RGD peptide in the bloodstream, allowing for more effective delivery to the target site. A promising strategy for attenuating the immune response is presented when RGD peptide‐based lipids are combined with immunosuppressive agents.…”

Lipid nanoparticles for antisense oligonucleotide gene interference into brain border-associated macrophages

“…In this work, we designed the synthesis procedure of the GR@LNPs to fulfill three principal terms: 1) to protect the GR payload at high compaction; 2) assist with cell uptake through the plasma membrane; and 3) release the GR payload into the cytosol at the highest transfection efficiency and the lowest toxicity as possible. Based on the aforementioned specifications, our formulation should include the following: a) ASO(GR), as an active pharmacological agent; b) cationic DChol lipid for ASO(GR) compaction under Manning condensation at 2: 1 stoichiometry; c) unsaturated phospholipid POPC, a bilayer former as a lipid stabilizer; d) lipid helper cholesterol, as a hydrophobic filler and stability enhancer; e) PEGylated phospholipid DSPE-PEG, as a surface cell adhesion additive (Noiri et al, 2019); f) functional phospholipid DSPE-PEG-MAN for specific anchoring to the mannose receptor of BAMs (MANr), selectively from associated microglia that do not express MANr (Galea et al, 2005). The wanted lamellar phase represents the densest state achievable inside the solid-like @LNP-core with a stoichiometry, corresponding to two lipid counterions (DChol) per base pair (i.e., GR:DChol 2 ).…”

Analysis of HepG2 cell response to a wide concentration range of mitomycin C using a multichannel quartz crystal microbalance system with a microscope

“…Therefore, QCM has been used for monitoring a chemical or biological phenomenon based on the resonant frequency change and resonant resistance or dumping factor [ [3] , [4] , [5] , [6] , [7] , [8] ]. QCM has also been used to examine the cell attachment process, focusing on the interaction between cultured cells and the surface of attached cells [ [9] , [10] , [11] , [12] , [13] , [14] , [15] , [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] ]. The effects of cell density [ [9] , [10] , [11] , [12] , [13] ] and species [ [14] , [15] , [16] ] on the change in the resonant frequency of a quartz crystal have been studied.…”