Influence of DNA condensation state on transfection efficiency in DNA/polymer complexes: An AFM and DLS comparative study

Volcke, Cédric; Pirotton, Sabine; Grandfils, Ch.; Humbert, Christophe; Thiry, P.A.; Ydens, Isabelle; Dubois, Philippe; Raes, Martine

doi:10.1016/j.jbiotec.2006.02.010

Cited by 44 publications

(30 citation statements)

References 40 publications

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“…Nucleic acid is delivered in the form of plasma, but increment of smaller oligomer is the main obstacle for extraploitation of gene therapies (Wu et al 2013;Volcke et al 2006). Both viral and nonviral vectors are included in gene delivery.…”

Section: Dendrimers In Gene Deliverymentioning

confidence: 99%

A review on comparative study of PPI and PAMAM dendrimers

et al. 2016

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Dendrimers are hyperbranched, monodispersed macromolecules with multivalent functional end groups. Dendrimers have been explored as carrier for many drugs like anticancer, antiviral, antimalarial, antiprotozoal, anti tubercular drugs. Although a number of different types of dendrimers containing different core molecules, branching monomers and surface functional groups have been designed till date for drug delivery applications, yet the poly(propyleneimine) (PPI) and poly(amidoamine) (PAMAM) dendrimers have been the most explored dendrimers in this regard. In this review, we have summarized a comparative data on PPI and PAMAM dendrimers particularly relevant to their properties, synthesis, toxicity, biomedical applications and drug delivery attributes.

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Section: Dendrimers In Gene Deliverymentioning

confidence: 99%

A review on comparative study of PPI and PAMAM dendrimers

et al. 2016

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“…Polyethylene glycol (PEG), which has the monomeric repeat unit [-CH 2 -CH 2 -O]-, is widely used in drug and gene delivery and has been incorporated into DNA complexes of several cationic polymers, including polymethacrylate [23], PEI [24][25][26][27][28][29][30][31][32], PLL [33][34][35] and poly(amidoamine)s [36]. PEG reduces the surface charge of the complexes [25,26,31,34], which in turn reduces cytotoxicity [25,27,31].…”

Section: Introductionmentioning

confidence: 99%

Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

2008

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Substrate-mediated gene delivery describes the immobilization of gene therapy vectors to a biomaterial, which enhances gene transfer by exposing adhered cells to elevated DNA concentrations within the local microenvironment. Surface chemistry has been shown to affect transfection by nonspecifically immobilized complexes using self-assembled monolayers (SAMs) of alkanethiols on gold. In this report, SAMs were again used to provide a controlled surface to investigate whether the presence of oligo(ethylene glycol) (EG) groups in a SAM could affect complex morphology and enhance transfection. EG groups were included at percentages that did not affect cell adhesion. Nonspecific complex immobilization to SAMs containing combinations of EG-and carboxylic acidterminated alkanethiols resulted in substantially greater transfection than surfaces containing no EG groups or SAMs composed of EG groups combined with other functional groups. Enhancement in transfection levels could not be attributed to complex binding densities or release profiles. Atomic force microscopy imaging of immobilized complexes revealed that EG groups within SAMs affected complex size and appearance and could indicate the ability of these surfaces to preserve complex morphology upon binding. The ability to control the morphology of the immobilized complexes and influence transfection levels through surface chemistry could be translated to scaffolds for gene delivery in tissue engineering and diagnostic applications.

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“…All images were recorded in air at room temperature, at a scan speed of 0.99 Hz. The background slope was resolved using first or second order polynomial functions [30].…”

Section: Methodsmentioning

confidence: 99%

Morphology, Structure and Function Characterization of PEI Modified Magnetic Nanoparticles Gene Delivery System

et al. 2014

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Modified magnetic nanoparticles are used as non-viral gene carriers in biological applications. To achieve successful gene delivery, it is critical that nanoparticles effectually assemble with nucleic acids. However, relatively little work has been conducted on the assemble mechanisms between nanoparticles and DNA, and its effects on transfection efficiency. Using biophysical and biochemical characterization, along with Atomic force microscopy (AFM) and Transmission electron microscopy (TEM), we investigate the morphologies, assembling structures and gene delivering abilities of the PEI modified magnetic nanoparticles (MNPs) gene delivery system. In this gene delivery system, MNP/DNA complexes are formed via binding of DNA onto the surface of MNPs. MNPs are favorable to not only increase DNA concentration but also prevent DNA degradation. Magnetofection experiments showed that MNPs has low cytotoxicity and introduces highly stable transfection in mammalian somatic cells. In addition, different binding ratios between MNPs and DNA result in various morphologies of MNP/DNA complexes and have an influence on transfection efficiency. Dose–response profile indicated that transfection efficiency positively correlate with MNP/DNA ratio. Furthermore, intracellular tracking demonstrate that MNPs move though the cell membranes, deliver and release exogenous DNA into the nucleus.

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Influence of DNA condensation state on transfection efficiency in DNA/polymer complexes: An AFM and DLS comparative study

Cited by 44 publications

References 40 publications

A review on comparative study of PPI and PAMAM dendrimers

A review on comparative study of PPI and PAMAM dendrimers

Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

Morphology, Structure and Function Characterization of PEI Modified Magnetic Nanoparticles Gene Delivery System

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