Influence of extraction solvent system on the extractability of lipid components from the biomass of Nannochloropsis gaditana

Ryckebosch, Eline; P.C., Bermudez, Sara,; Termote‐Verhalle, Romina; Bruneel, Charlotte; Muylaert, Koenraad; Parra‐Saldívar, Roberto; Foubert, Imogen

doi:10.1007/s10811-013-0189-y

Cited by 75 publications

(54 citation statements)

References 38 publications

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“…The extraction with ethanol (96% v/v) and the posterior purification of this extract with hexane has been used in previous works treatment was applied to the lipids extracted with hexane, the polar lipid content did not decrease and the SL purity only increased from 56% to 61%. This result indicates that the lipids previously extracted with hexane contained a low or negligible amount of waxes and gums susceptible to precipitation in acetone at low temperature.These yields are also similar to the ones obtained byRyckebosch et al (2014a). the polar lipids and increase the NSL content of the hexanic phase.…”

supporting

confidence: 88%

Extraction of microalgal lipids and the influence of polar lipids on biodiesel production by lipase-catalyzed transesterification

López

Medina

Moreno

et al. 2016

Bioresource Technology

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supporting

confidence: 88%

Extraction of microalgal lipids and the influence of polar lipids on biodiesel production by lipase-catalyzed transesterification

López

Medina

Moreno

et al. 2016

Bioresource Technology

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“…So, by using hexane, not only were the 68.6% of NSLs contained in the biomass recovered but also the 27.7% of polar lipids (Table 2B), an excessive amount when considering the objective of this work. Similar results were obtained by Ryckebosch et al (2014a) from different microalgal species (Isochrysis galbana, Nannochloropsis gaditana, Nannochloropsis sp., and Phaedactylum tricornutum) testing several extraction solvent systems. For all the microalgae tested, the neutral lipid (NL) content in the hexane extract was higher than the content in the original biomass; for example, the hexane extract from Nannochloropsis sp.…”

Section: Simultaneous Extraction and Fractionation Of Microalgal Lipisupporting

confidence: 86%

Simultaneous extraction and fractionation of lipids from the microalga Nannochloropsis sp. for the production of EPA-rich polar lipid concentrates

et al. 2020

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There is broad scientific evidence on the health benefits of omega-3 polyunsaturated fatty acids (n-3 PUFAs), especially docosahexaenoic acid (DHA, 22:6n3) and eicosapentaenoic acid (EPA, 20:5n3). On the other hand, polar lipids (phospholipids, PLs, and glycolipids, GLs) are excellent emulsifying agents, making them useful both as food products and as excipients for drugs and cosmetics. The bioavailability of n-3 PUFAs in the form of PLs is greater than that of ethyl esters and triacylglycerols. This work has developed an easy method for the simultaneous extraction and fractionation of saponifiable lipids (SLs) from the microalga Nannochloropsis sp., using low-toxicity solvents. Firstly, a lipidic fraction very rich in neutral saponifiable lipids (NSLs) was obtained using hexane. Next, an EPA and polar lipids (PLs and GLs)-enriched fraction was obtained using ethanol (96%). Under optimal conditions, in the first extraction with hexane, an SL extract with 86.3% NSLs was obtained; this extract contained 88.9% of the biomass NSLs. Subsequently, in the second extraction step with ethanol (96%), an SL extract with 87.1% polar lipids and up to 35.2% EPA was obtained. This SL extract contained 87.1% of the polar lipids and 74.7% of the EPA from the Nannochloropsis sp. biomass.

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“…However, this study found dichloromethane to have a better ability in concentrating fucoxanthin. Ryckebosch et al [44] supported the use of dichloromethane compared to hexane, ethyl acetate or acetone as it could recover higher total lipids, especially semi-polar lipids (glycolipids and phospholipids) in microalgae. This study recommends the use of dichloromethane in future TLC approaches for fucoxanthin fractionation and isolation.…”

Section: Solvent Systemsmentioning

confidence: 99%

Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968

et al. 2015

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Fucoxanthin is the major carotenoid produced by diatoms and shown to exhibit potent anti-obesity and anticancer activities. This study presents a simple protocol for the preparation of fucoxanthin-rich fraction (FxRF) with high antioxidative properties from a selected marine diatom, Chaetoceros calcitrans. Fucoxanthin concentrates were prepared from crude methanolic extract (CME) with liquid-liquid partitioning of three types of solvent systems (i.e. diethyl ether-water, dichloromethane-water and ethyl acetate-water), followed by comparative antioxidant evaluation. Dichloromethane fraction (DCMF) was found to contain significantly higher (p b 0.05) level of carotenoid (7.13 ± 0.01 mg·g −1 DW) and fucoxanthin (5.25 ± 0.03 mg FX·g −1 DW) contents accompanied with elevated (p b 0.05) antioxidant activities (DPPH• and ABTS• + radical scavenging, and beta carotene bleaching assays) as compared to other tested fractions. This study showed that FxRF with enhanced antioxidant properties could be effectively produced and concentrated through the DCM-H 2 O partition of CME.

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Influence of extraction solvent system on the extractability of lipid components from the biomass of Nannochloropsis gaditana

Cited by 75 publications

References 38 publications

Extraction of microalgal lipids and the influence of polar lipids on biodiesel production by lipase-catalyzed transesterification

Extraction of microalgal lipids and the influence of polar lipids on biodiesel production by lipase-catalyzed transesterification

Simultaneous extraction and fractionation of lipids from the microalga Nannochloropsis sp. for the production of EPA-rich polar lipid concentrates

Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968

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