Influence of gibberellic acid on the incorporation of 8-14C adenine into adenosine 3′,-5′-cyclic phosphate in barley aleurone layers

Pollard, Clifford J.

doi:10.1016/0304-4165(70)90176-5

Cited by 76 publications

(24 citation statements)

References 5 publications

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“…As an example of the former, Salomon & Mascarenhas (1971) reported that cAMP delayed petiole abscission in Coleus, but did not demonstrate that this was a process in vivo, merely that the cyclic nucleotide could replicate the action of auxin. In the latter category, Pollard (1970) obtained a radiolabelled product from the incubation of [8-"%C] adenine with germinating barley seeds that chromatographed together with cAMP in ten chromatographic systems. The latter report was criticized on the basis that the chromatographic systems would not resolve the putative secondary messenger cAMP from the RNA catabolism intermediate, adenosine 2h,3h-cyclic monophosphate.…”

Section:       C   mentioning

confidence: 99%

“…Early papers (Pollard, 1970 ;Oota, 1972 ;Keates, 1973 ;Truelsen et al, 1974) were based on observations of various physiological and metabolic responses after the exogenous application of cAMP, cAMP analogues and phosphodiesterase inhibitors known at that time. This shotgun-like approach was performed without any knowledge of the underlying metabolism in plants.…”

Section:       C   mentioning

confidence: 99%

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Tansley Review No. 106

et al. 1999

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 For three decades, hypotheses relating to the occurrence and function of cyclic nucleotides in higher plants have been highly controversial. Although cyclic nucleotides had been shown to have key regulatory roles in animals and bacteria, investigations with higher plants in the 1970s and early 1980s were criticized on the basis of (i) a lack of specificity of effects apparently elicited by cyclic nucleotides, (ii) the equivocal identification of putative endogenous cyclic nucleotides and (iii) ambiguity in the identification of enzymes connected with cyclic nucleotide. More recent evidence based on more rigorous identification procedures has demonstrated conclusively the presence of cyclic nucleotides, nucleotidyl cyclases and cyclic nucleotide phosphodiesterases in higher plants, and has identified plant processes subject to regulation by cyclic nucleotides. Here we review the history of the debate, the recent evidence establishing the presence of these compounds and their role ; future research objectives are discussed.

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Section:       C   mentioning

confidence: 99%

Section:       C   mentioning

confidence: 99%

Tansley Review No. 106

et al. 1999

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“…Through the work of a number of different investigators, it became evident that cAMP promotes activation of catabolite-repressible proteins by a mechanism which differs fundamentally from protein kinase activation mechanisms in higher organisms , 1970Pastan, 1968, 1971;Zubay, et al, 1970;Anderson, et al, 1971;Pastan, et al, 1971;Beckwith, et al, 1972;Gondo, et al, 1978). In microbial cells, cAMP forms a complex with a specific protein dimer present in the cytoplasm, termed "cAMP receptor protein", or "CRP".…”

Section: Involvement Of Camp In Heterotrophic Organismsmentioning

confidence: 99%

“…To the casual reader of the literature, it would appear that cAMP has unequivocably been shown to occur in higher plants and that it has numerous functions in these organisms. However, the Labeling techniques using ^C-adenine (Pollard, 1970), the Gilman protein binding assay , and the protein kinase stimulation assay have been used to assay plant materials for cAMP (reviewed by Ararhein, 1977). These techniques, although suitable for the assay of cAMP from heterotrophic organisms, produce positive but erroneous results in plant extracts in the absence of rigorous purification techniques , Despite several attempts to isolate it, adenyl cyclase activity has not yet been convincingly demonstrated in any tissue of higher plants (Robison, et al, 1971a;Lin and Varner, 1972).…”

Section: Involvement Of Camp In Photosynthetic Organismsmentioning

confidence: 99%

Investigation on the occurrence and significance of cyclic adenosine 3':5'-monophosphate in phytoplankton and natural aquatic communities

Francko¹

1980

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“…Other reports have presented evidence for a change in cyclic AMP levels after treatment of plant tissues with gibberellic acid (7), auxin (9), or irradiation with far red (3).…”

Section: )mentioning

confidence: 99%

Failure to Detect Cyclic 3′, 5′-Adenosine Monophosphate in Healthy and Crown Gall Tumorous Tissues of Vicia faba

Niles¹,

Mount²

1974

Plant Physiol.

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Attempts were made to provide proof for the occurrence of cyclic 3', 5'-adenosine monophosphate in healthy and crown gall tissues of Vicia faba. Although our purified extracts gave positive readings in the Gilman binding assay for cyclic AMP, they were not digested by a specific cyclic 3', 5'-adenosine monophosphate phosphodiesterase from beef heart. The extracts were digested, however, by a partially purified cyclic nucleotide phosphodiesterase from carrot tissue, which attacks both cyclic 2',3'-and 3', 5'-nucleotides. The data indicate that the substances detected in the V. faba extracts are perhaps cyclic 2',3'-nucleotides, a possible RNA degradation product.Various laboratories have claimed they have detected cyclic 3', 5'-adenosine monophosphate in higher plant tissues (8, 1 1). Other reports have presented evidence for a change in cyclic AMP levels after treatment of plant tissues with gibberellic acid (7), auxin (9), or irradiation with far red (3).A cyclic nucleotide phosphodiesterase has been isolated and partially purified from a number of plant tissues (4,6,10 AMP recovery after purification. After homogenization for 1 min at high speed in a Waring Blendor, the extract was clarified by squeezing through four layers of cheesecloth. The filtrate was then boiled for 17 min, neutralized, and reboiled for 17 min. This step precipitated almost all the protein and polysaccharides. The pellet obtained by centrifugation (10,OOOg for 15 min) was saved for protein determination, while the supernatant extracts were acidified and purified by passage through 15 x 0.7 cm columns of Dowex AG-50-X8 and the cyclic AMP fraction eluted with 0.05 N HCI. This eluate was neutralized in 0.01 M tris-HCl, pH 7.4, and was applied to a second column (5 X 0.7 cm of Dowex-2 equilibrated with 0.01 M tris-HCI, pH 7.4), and the cyclic AMP fraction again eluted with 0.05 N HCI. Dowex-2 eluates were lyophilized; the residues were dissolved in 0.01 M tris-HCl, pH 7.4, and the pH was adjusted to 7.4 with 0.1 N NaOH. Recoveries were routinely 50 to 60%.Duplicate samples from each treatment and from column blanks were assayed for cyclic AMP by the method of Gilman (2). The experimental results are expressed as picomoles of cyclic AMP/mg protein + the standard error of the mean determined from three to seven separate experiments.

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Influence of gibberellic acid on the incorporation of 8-14C adenine into adenosine 3′,-5′-cyclic phosphate in barley aleurone layers

Cited by 76 publications

References 5 publications

Tansley Review No. 106

Tansley Review No. 106

Investigation on the occurrence and significance of cyclic adenosine 3':5'-monophosphate in phytoplankton and natural aquatic communities

Failure to Detect Cyclic 3′, 5′-Adenosine Monophosphate in Healthy and Crown Gall Tumorous Tissues of Vicia faba

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