Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells

Abstract

Stress conditioning (e.g., thermal, shear, and tensile stress) of bone cells has been shown to enhance healing. However, prior studies have not investigated whether combined stress could synergistically promote bone regeneration. This study explored the impact of combined thermal and tensile stress on the induction of heat shock proteins (HSPs) and bone-related proteins by a murine preosteoblast cell line (MC3T3-E1). Cells were exposed to thermal stress using a water bath (44°C for 4 or 8 minutes) with postheating incubation (37°C for 4 hours) followed by exposure to cyclic strain (equibiaxial 3%, 0.2 Hz, cycle of 10-second tensile stress followed by 10-second rest). Combined thermal stress and tensile stress induced mRNA expression of HSP27 (1.41 relative fold induction (RFI) compared to sham-treated control), HSP70 (5.55 RFI), and osteopontin (1.44 RFI) but suppressed matrix metalloproteinase-9 (0.6 RFI) compared to the control. Combined thermal and tensile stress increased vascular endothelial growth factor (VEGF) secretion into the culture supernatant (1.54-fold increase compared to the control). Therefore, combined thermal and mechanical stress preconditioning can enhance HSP induction and influence protein expression important for bone tissue healing.

Adjuvant Transgingival Therapy with Visible Light Plus Water-Filtered Infrared-A (VIS + wIRA) in Periodontal Therapy—A Randomized, Controlled, Stratified, Double-Blinded Clinical Trial

“…Studies have shown that fibroblasts and endothelial cells exposed to mild temperature stress (1 h at 41 °C) demonstrated slowed cell aging and an accelerated angiogenesis rate (Rattan et al, 2007). Furthermore, direct application of heat positively affects bone regeneration and mineralization and increases osteogenetic differentiation [ 19 , 20 , 28 ].…”

“…Further, fibroblasts and endothelial cells exposed to mild temperature stress (1 h at 41 °C) showed slowed cell ageing and an accelerated angiogenesis rate [ 18 ]. Direct application of heat also positively affects bone regeneration and mineralization [ 19 , 20 ].…”

Osteoking improves OP rat by enhancing HSP90‑β expression

“…High expressions of HSP90-β and BMP-2 was a common phenomenon of physical treatments for OP. HSP90-β promotes osteogenesis by enhancing BMP-2 (43,44).…”

Compression loading-induced stress responses in intervertebral disc cells encapsulated in 3D collagen constructs

“…Mechanical loading may be another environmental stressor to cells especially for the cells that are found in the load bearing tissues. Hence, musculoskeletal cells such as chondrocytes, tendon fibroblasts and pre-osteoblasts, were shown to respond to mechanical stress by upregulating the expression of heat shock proteins (HSPs) 2 3 4 5 6 7 8 . Similarly, in cartilage tissues and osteocytes, ER stress markers were upregulated in response to mechanical stress 9 10 11 .…”