Influence of lipid composition on the thermotropic behavior and size distribution of mixed cationic liposomes

Barenholz, Yechezkel; Bombelli, Cecilia; Bonicelli, M.G.; Profio, Pietro Di; Giansanti, Luisa; Mancini, Giovanna; Pascale, F

doi:10.1016/j.jcis.2010.11.062

Cited by 32 publications

(24 citation statements)

References 28 publications

(30 reference statements)

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“…Cationic liposome is a promising non-virus gene vehicle (15,16), whose membrane property confers various advantages in application terms of therapy and study of gene transfection, including maintaining the biological activity of genes, protecting genes from degradation by lysosomes. It is also non-immunogenic, easy to apply, with high repeatability and it is naturally degraded (17)(18)(19)(20)(21)(22)(23)(24)(25). The current study used Lipofectamine ® 2000 to transfect A549 human alveolar type II epithelial cell line with HSP27-interfering plasmid; the procedure was easy, safe and reliable with high transfection efficacy, and met the requirement of subsequent experiments.…”

Section: Discussionmentioning

confidence: 97%

“…Initially, two aliquots of 3 ml Opti-MEM medium was added into two 5 ml Eppendorf micro test tubes, Lipofectamine ® 2000 and HSP27 plasmid were added individually into the two tubes and mixed with OPTI-MEM medium. A series of Lipofectamine ® 2000 volumes (20,25,30,20, and 20 µl) and HSP27 plasmid (8, 8, 8, 9 and 10 µg) were correspondingly tested. The reagents were individually incubated at 37˚C for 10 min prior being mixed and incubated at 37˚C for an additional 20 min.…”

Section: Reagents and Materialsmentioning

confidence: 99%

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Effect of liposome‑mediated HSP27 transfection on collagen synthesis in alveolar type II epithelial cells

et al. 2018

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To investigate the effect of liposome Lipofectamine® 2000‑mediated HSP27 plasmid transfection in A549 human alveolar type II epithelial cell line on collagen synthesis during transforming growth factor‑β1 (TGF‑β1)‑induced type II epithelial cell transition to myofibroblasts. Cells were transfected with varying ratios of the Lipofectamine® 2000‑mediated heat shock protein 27 (HSP27) plasmid and the transfection efficiency was determined using flow cytometry. The maximum transfection efficacy was confirmed by laser confocal microscopy. HSP gene expression and the most efficient HSP27 plasmid were determined using reverse transcription‑quantitative polymerase chain reaction. Western blot analysis was used to examine HSP27 and collagen expression levels. With a transfection efficiency of 83%, the 8 µg:20 µl ratio of liposome: Plasmid had the highest transfection levels. Among the four different interference sequences in the HSP27 plasmid, the D sequence had the highest interference effect with 70% silencing of the HSP27 gene. The expression of type I and III collagen in TGF‑β1‑induced transition of A549 human alveolar type II epithelial cell line to myofibroblasts was significantly downregulated by the successful transfection with HSP27‑interfering plasmid. The expression of type I and III collagen in the TGF‑β1‑induced transition of A549 cells to myofibroblasts was significantly downregulated by transfection of A549 cells with HSP27 plasmid D‑interfering sequence and optimal ratio of Lipofectamine® 2000 and HSP27 plasmid.

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Section: Discussionmentioning

confidence: 97%

Section: Reagents and Materialsmentioning

confidence: 99%

Effect of liposome‑mediated HSP27 transfection on collagen synthesis in alveolar type II epithelial cells

et al. 2018

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“…At molar ratios DPPC:erythrodiol of 17 and 34 %, a splitting of the transition peak is produced indicating the disruption of the ordering of the alkyl chains. The decrease of the transition enthalpy in the presence of a drug is usually attributed to the drug interaction with the hydrophobic chains of the bilayer (Detoni et al 2009;Koukoulitsa et al 2011;Potamitis et al 2011;Barenholz et al 2011). Compared to other pentacyclic triterpenes (Prades et al 2011) which did not affect significantly the melting temperature and the enthalpy of the main phase transition, erythrodiol produced remarkable decrease of the enthalpy values of the main peak (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Effect of Erythrodiol, A Natural Pentacyclic Triterpene from Olive Oil, on the Lipid Membrane Properties

et al. 2015

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The effect of erythrodiol, a natural pentacyclic triterpene to which humans are exposed through nutrients, on the lipid membranes is studied using liposomes as a membrane model. Empty and erythrodiol-loaded liposomes were prepared by the reverse phase evaporation method followed by the extrusion and by the thin film hydration method. Liposomes were characterized in terms of size and zeta potential and were imaged by transmission electron microscopy (TEM) and atomic force microscopy (AFM). The effect of erythrodiol on thermotropic behavior of DPPC bilayers is also examined by differential scanning calorimetry (DSC). The DSC thermograms suggested that erythrodiol interacted with the polar head groups of phospholipids and may produce a disruption of the ordering of the alkyl chains. The diffraction light scattering analysis showed that erythrodiol-loaded liposomes presented a decrease in the vesicle size when compared to blank liposomes. Images obtained by TEM confirmed the formation of unilamellar and spherical liposomes. AFM images showed spherical vesicles and single lipid bilayers. The latter were more abundant in the preparations containing erythrodiol than in the blank ones. Moreover, erythrodiol-loaded liposomes tended to rupture into single lipid bilayers during scanning. The study may provide a better understanding of pentacyclic triterpenes-membrane interaction.

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“…It has also been reported that niosomes containing monoalkyl surfactants from methylprolinolinium show good antimicrobial activity while niosomes formulated with the dialkyl homologue do not show biological activity. These authors suggested that the lateral homogeneity of the niosomes´ bilayer could be another important parameter that controls their biological activity [38][39]. Whereas niosomes prepared with single chain surfactants had Tm values around 27 ªC, double chain cationic surfactants gave rise to niosomes with a high Tm (40 ºC).…”

Section: Antimicrobial Activitymentioning

confidence: 99%

New cationic vesicles prepared with double chain surfactants from arginine: Role of the hydrophobic group on the antimicrobial activity and cytotoxicity

Pinazo

Petrizelli

Bustelo

et al. 2016

Colloids and Surfaces B: Biointerfaces

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Influence of lipid composition on the thermotropic behavior and size distribution of mixed cationic liposomes

Cited by 32 publications

References 28 publications

Effect of liposome‑mediated HSP27 transfection on collagen synthesis in alveolar type II epithelial cells

Effect of liposome‑mediated HSP27 transfection on collagen synthesis in alveolar type II epithelial cells

Effect of Erythrodiol, A Natural Pentacyclic Triterpene from Olive Oil, on the Lipid Membrane Properties

New cationic vesicles prepared with double chain surfactants from arginine: Role of the hydrophobic group on the antimicrobial activity and cytotoxicity

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