Influence of mobile phase composition on the high‐performance liquid chromatographic/electrospray ionization mass spectrometric analysis of 11‐nor‐9‐carboxy‐Δ⁹‐tetrahydrocannabinol (THC‐COOH) and its glucuronide in urine

Abstract

Influence of mobile phase composition on the high-performance liquid chromatographic/electrospray ionization mass spectrometric analysis of 11-nor-9-carboxy-1 9 -tetrahydrocannabinol (THC-COOH) and its glucuronide in urine

Chemistry and Biological Activity of Tetrahydrocannabinol and its Derivatives

“…Nonetheless, the usage of HPLC becomes more and more of interest in this field especially in combination with MS [115][116][117][118][119][120]. Besides the usage of deuterated samples as internal standards Fisher et al [121] describe the use of a dibrominated THC-COOH (see 7.5).…”

Analytical approaches for the determination of phytocannabinoids and endocannabinoids in human matrices

“…[40,56,121,122] For the analysis, samples are often submitted to enzymatic cleavage of glucuronic acid. Basic hydrolysis with NaOH, [123][124][125][126][127] KOH, [12,31,122,[128][129][130] or rarely with NH 3 , [131] has lower cost and time and is the most widespread. [41,56,117,121,123] An advantage of simultaneously detecting conjugated and parent compounds is that their ratio may be used for the identification of recent cannabis intake in a single urine specimen; however, additional studies must be performed to confirm this hypothesis.…”

“…Cleavage of the glucuronic bond can be accomplished by basic or enzymatic hydrolysis. Basic hydrolysis with NaOH, [123][124][125][126][127] KOH, [12,31,122,[128][129][130] or rarely with NH 3 , [131] has lower cost and time and is the most widespread. Enzymatic cleavage may include the use of β-glucuronidase derived from different species, including bacteria, mollusks, cattle, and limpets.…”