Influence of Nanoparticle Shape, Size, and Surface Functionalization on Cellular Uptake

Ma, Ningning; Ma, Chao; Li, Chuanyan; Wang, Ting; Tang, Yongjun; Wang, Hongyin; Moul, Xianbo; Chen, Zhan; Hel, Nongyue

doi:10.1166/jnn.2013.7525

Cited by 168 publications

(108 citation statements)

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“…SiNPs were internalized into cells via endocytosis. 33,39,40 The TEM observation, as shown in Figure 2A, provided evidence of uptake of SiNPs in L-02 and HepG2 cells, showing that SiNPs were mainly distributed in cytoplasm, lysosomes, and autophagic vacuoles. Then we observed that SiNPs decreased cell viability of L-02 and HepG2 cells in a dose-and time-dependent manner.…”

Section: Discussionmentioning

confidence: 97%

Silica nanoparticles induce autophagy dysfunction via lysosomal impairment and inhibition of autophagosome degradation in hepatocytes

Wang

Lu³

et al. 2017

IJN

172

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Autophagy dysfunction is considered as a potential toxic mechanism of nanomaterials. Silica nanoparticles (SiNPs) can induce autophagy, but the specific mechanism involved remains unclear. Therefore, the aim of this study was to confirm the effects of SiNPs on autophagy dysfunction and explore the possible underlying mechanism. In this article, we reported that cell-internalized SiNPs exhibited dose- and time-dependent cytotoxicity in both L-02 and HepG2 cells. Multiple methods verified that SiNPs induced autophagy even at the noncytotoxic level and blocked the autophagic flux at the high-dose level. Notably, SiNPs impaired the lysosomal function through damaging lysosomal ultrastructures, increasing membrane permeability, and downregulating the expression of lysosomal proteases, cathepsin B, as evidenced by transmission electron microscopy, acridine orange staining, quantitative reverse transcription-polymerase chain reaction, and Western blot assays. Collectively, these data concluded that SiNPs inhibited autophagosome degradation via lysosomal impairment in hepatocytes, resulting in autophagy dysfunction. The current study not only discloses a potential mechanism of autophagy dysfunction induced by SiNPs but also provides novel evidence for the study of toxic effect and safety evaluation of SiNPs.

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Section: Discussionmentioning

confidence: 97%

Silica nanoparticles induce autophagy dysfunction via lysosomal impairment and inhibition of autophagosome degradation in hepatocytes

Wang

Lu³

et al. 2017

IJN

172

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“…66 A review of the literature revealed that nonspherical NPs show great promise as cancer drugdelivery vectors, such as filamentous, wormlike micelles, or needle-like morphologies. This may be explained with reference to facilitated adhesion of NPs on cell membranes and subsequent facilitated endocytosis.…”

Section: Impact Of Physicochemical Properties On Targeting Efficiencymentioning

confidence: 99%

Multifaceted applications of bile salts in pharmacy: an emphasis on nanomedicine

Elnaggar¹

2015

IJN

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The human body has long provided pharmaceutical science with biomaterials of interesting applications. Bile salts (BSs) are biomaterials reminiscent of traditional surfactants with peculiar structure and self-assembled topologies. In the pharmaceutical field, BSs were employed on the basis of two different concepts. The first concept exploited BSs’ metabolic and homeostatic functions in disease modulation, whereas the second one utilized BSs’ potential to modify drug-delivery characteristics, which recently involved nanotechnology. This review is the first to gather major pharmaceutical applications of BSs from endogenous organotropism up to integration into nanomedicine, with a greater focus on the latter domain. Endogenous applications highlighted the role of BS in modulating hypercholesterolemia and cancer therapy in view of enterohepatic circulation. In addition, recent BS-integrated nanomedicines have been surveyed, chiefly size-tunable cholate nanoparticles, BS-lecithin mixed micelles, bilosomes, probilosomes, and surface-engineered bilosomes. A greater emphasis has been laid on nanosystems for vaccine and cancer therapy. The comparative advantages of BS-integrated nanomedicines over conventional nanocarriers have been noted. Paradoxical effects, current pitfalls, future perspectives, and opinions have also been outlined.

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“…It is known that, whereas the free drug enters cells by simple diffusion, drug-loaded NPs may provide a different cell entry mechanism such as endocytosis, pinocytosis, or phagocytosis, which is still under discussion. 48,49 It has been suggested that drug-loaded NPs are too large to pass through both the plasma and nuclear membranes. Thus, the presence of the drug within the cell nucleus must occur only once it has been released from inside the NPs.…”

Section: 31mentioning

confidence: 99%

Enhanced antitumor activity of doxorubicin in breast cancer through the use of poly(butylcyanoacrylate) nanoparticles

Cabeza¹,

Ortíz²,

Arias³

et al. 2015

IJN

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The use of doxorubicin (DOX), one of the most effective antitumor molecules in the treatment of metastatic breast cancer, is limited by its low tumor selectivity and its severe side effects. Colloidal carriers based on biodegradable poly(butylcyanoacrylate) nanoparticles (PBCA NPs) may enhance DOX antitumor activity against breast cancer cells, thus allowing a reduction of the effective dose required for antitumor activity and consequently the level of associated toxicity. DOX loading onto PBCA NPs was investigated in this work via both drug entrapment and surface adsorption. Cytotoxicity assays with DOX-loaded NPs were performed in vitro using breast tumor cell lines (MCF-7 human and E0771 mouse cancer cells), and in vivo evaluating antitumor activity in immunocompetent C57BL/6 mice. The entrapment method yielded greater drug loading values and a controlled drug release profile. Neither in vitro nor in vivo cytotoxicity was observed for blank NPs. The 50% inhibitory concentration (IC 50 ) of DOX-loaded PBCA NPs was significantly lower for MCF-7 and E0771 cancer cells (4 and 15 times, respectively) compared with free DOX. Furthermore, DOX-loaded PBCA NPs produced a tumor growth inhibition that was 40% greater than that observed with free DOX, thus reducing DOX toxicity during treatment. These results suggest that DOX-loaded PBCA NPs have great potential for improving the efficacy of DOX therapy against advanced breast cancers.

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Influence of Nanoparticle Shape, Size, and Surface Functionalization on Cellular Uptake

Cited by 168 publications

References 0 publications

Silica nanoparticles induce autophagy dysfunction via lysosomal impairment and inhibition of autophagosome degradation in hepatocytes

Silica nanoparticles induce autophagy dysfunction via lysosomal impairment and inhibition of autophagosome degradation in hepatocytes

Multifaceted applications of bile salts in pharmacy: an emphasis on nanomedicine

Enhanced antitumor activity of doxorubicin in breast cancer through the use of poly(butylcyanoacrylate) nanoparticles

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