Influence of nutrients on the bioaccessibility and transepithelial transport of polybrominated diphenyl ethers measured using an in vitro method and Caco-2 cell monolayers

Li, Xiaojing; Wang, Mengmeng; Yang, Yan; Lei, Bingli; Ma, Shengtao; Yu, Yingxin

doi:10.1016/j.ecoenv.2020.111569

Cited by 16 publications

(16 citation statements)

References 63 publications

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“…A total of 150 µL was sampled each time. Meanwhile, 150 µL of HBSS solution was added correspondingly at 37 °C (Li et al ., 2021). The ACE inhibitory peptide content of each sample was determined by HPLC according to the method described in section ‘Determination of the ACE inhibition ratio and IC50 value’.…”

Section: Methodsmentioning

confidence: 99%

Improved stability and transshipment of enzymatic hydrolysate with ACE inhibitory activity‐loaded nanogels based on glycosylated soybean protein isolate via the Maillard reaction

Cui

Jia

Shi

et al. 2021

Int J of Food Sci Tech

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Summary In this study, soybean protein isolate (SPI)‐konjac glucomannan (KGM) conjugates were fabricated via the Maillard reaction and their potential to improve enzymatic hydrolysate with ACE inhibitory activity stability was assessed. Briefly, we loaded the enzymatic hydrolysate into SPI‐KGM conjugates using a pH‐driven method. Thereafter, the transport pathway of enzymatic hydrolysate with angiotensin‐converting enzyme (ACE) inhibitory activity SPI‐KGM (ACE‐SPI‐KGM) core–shell nanogels was investigated using CaCo‐2 cell monolayer. The results of microstructure, encapsulation efficiency, ACE inhibition ratio and IC50 value verified that the nanogels displayed a spherical structure and excellent physicochemical properties. The ζ‐potential, Z‐average diameter and polydispersity index demonstrated that the stability of the core–shell nanogels was significantly superior to that of the enzymatic hydrolysate. In addition, the transport of the enzymatic hydrolysate in nanogels onto the CaCo‐2 cell monolayer revealed a higher transport capacity and was dominated by cell bypass transport. In summary, the SPI‐KGM conjugates could serve as potential as delivery systems for enzymatic hydrolysate with ACE inhibitory activity to fortify functional foods and medicines.

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Section: Methodsmentioning

confidence: 99%

Improved stability and transshipment of enzymatic hydrolysate with ACE inhibitory activity‐loaded nanogels based on glycosylated soybean protein isolate via the Maillard reaction

Cui

Jia

Shi

et al. 2021

Int J of Food Sci Tech

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show abstract

“…Moreover, these cells’ morphology is very similar to human intestinal epithelial cells, and they have same cellular polarity. Therefore, Caco-2 cells can be used as a model to study the intestinal permeability and transport mechanism of liposomes [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Caco-2 Cell Transport Assay of Chito-Oligosaccharides Nano-Liposomes Based on Layer-by-Layer Coated

et al. 2021

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Chito-oligosaccharides (COSs) were encapsulated by the film-ultrasonic method into three nano-liposomes, which were uncoated liposomes (COSs-Lip), chitosan-coated liposomes (CH-COSs-Lip), and sodium alginate (SA)/chitosan (CH)-coated liposomes (SA/CH-COSs-Lip). The physicochemical and structural properties, as well as the stability and digestive characteristics, of all three nano-liposomes were assessed in the current study. Thereafter, the characteristics of intestinal absorption and transport of nano-liposomes were investigated by the Caco-2 cell monolayer. All nano-liposomes showed a smaller-sized distribution with a higher encapsulation efficiency. The ζ-potential, Z-average diameter (Dz), and polydispersity index (PDI) demonstrated that the stability of the SA/CH-COSs-Lip had much better stability than COSs-Lip and CH-COSs-Lip. In addition, the transport of the nano-liposomes via the Caco-2 cell monolayer indicated a higher transmembrane transport capacity. In summary, the chitosan and sodium alginate could serve as potential delivery systems for COSs to fortify functional foods and medicines.

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“…Os compostos fenólicos são muito heterogêneos e devido a isso, eles são subdivididos em classes, sendo as cinco principais conhecidas como: ácidos fenólicos, flavonoides, lignanas, taninos e estilbenos (BODOIRA; MAESTRI, 2020;GE;LI;LISAK, 2020;MULLER et al, 2019).…”

Section: 4 24 Compostos Fenólicosunclassified

“…Essa classe de compostos tem chamado muito a atenção das indústrias de alimentos e até das farmacêuticas, devido ao potencial bioativo destas moléculas, com destaque a atividade antioxidante (GE;LI;LISAK, 2020;KUMAR;GOEL, 2019;LIU et al, 2019).…”

Section: 4 24 Compostos Fenólicosunclassified

“…Em relação ao uso de culturas celulares para ensaio de biodisponibilidade, a linhagem de células Caco-2, obtidas de adenocarcinoma de cólon humano, vêm sendo proposta como um promissor modelo de transporte epitelial de compostos por diversos autores (HUBATSCH; RAGNARSSON; ARTURSSON, 2007;LI et al, 2021;VILLALVA et al, 2018). Por se tratarem de células que quando cultivadas em monocamadas possuem a funcionalidade de se diferenciarem em células semelhantes aos enterócitos, com microvilosidades na região apical, as células Caco-2 se comporta como potenciais células absortivas (LI et al, 2021).…”

Section: 5 25 Biodisponibilidade Bioacessibilidade E Bioatividadeunclassified

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Digestão >i<in vitro>/i< e transporte de polifenóis de própolis orgânica do Sul do Brasil por mmeio de células humanas Caco-2

Saliba¹

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Influence of nutrients on the bioaccessibility and transepithelial transport of polybrominated diphenyl ethers measured using an in vitro method and Caco-2 cell monolayers

Cited by 16 publications

References 63 publications

Improved stability and transshipment of enzymatic hydrolysate with ACE inhibitory activity‐loaded nanogels based on glycosylated soybean protein isolate via the Maillard reaction

Improved stability and transshipment of enzymatic hydrolysate with ACE inhibitory activity‐loaded nanogels based on glycosylated soybean protein isolate via the Maillard reaction

Characterization and Caco-2 Cell Transport Assay of Chito-Oligosaccharides Nano-Liposomes Based on Layer-by-Layer Coated

Digestão >i<in vitro>/i< e transporte de polifenóis de própolis orgânica do Sul do Brasil por mmeio de células humanas Caco-2

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