Influence of Operating Parameters on the Use of the Microwave-Assisted Process (MAP) for the Extraction of Azadirachtin-Related Limonoids from Neem (<i>Azadirachta indica</i>) under Atmospheric Pressure Conditions

Dai, Jingjie; Yaylayan, V.A.; Raghavan, G.S.V.; Paré, J. R. Jocelyn; Liu, Z.; Bélanger, Jacqueline M.R.

doi:10.1021/jf010592k

Cited by 44 publications

(18 citation statements)

References 14 publications

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“…It has been demonstrated that microwave energy had the capacity of enhancing the extraction yields of the target compounds as opposed to conventional methods (Dai et al 1999(Dai et al , 2001Martino et al 2006;Nemes 2007;Williams et al 2004;Yang et al 2004). The enhancing effect of microwaves is due to the interactions between the electric component of the microwave field with the dipolar molecules and the ionic species present in the extraction mixtures (solvent/reagent+sample; Meda et al 2005;Orsat et al 2005;Raghavan et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Microwave-Assisted Extraction of Secoisolariciresinol Diglucoside—Method Development

Nemes

Orsat

2009

Food Bioprocess Technol

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An optimized microwave-assisted extraction (MAE) method was developed for extracting secoisolariciresinol diglucoside (SDG) from flaxseed. This paper presents the optimization of factors for maximizing the extraction yield of SDG. This work was conducted using the experimental domain identified in a previous study by means of screening designs, that is, samples of 1 g defatted flaxseed meal (DFM) were extracted with 50 ml NaOH of concentration of 0.5-1 M, at microwave power levels of 60-360 W, for 3-9 min, with the microwave power applied intermittently (power on 30 s/min) and continuously (power on 60 s/min). The MAE of SDG was maximized when 1 g DFM was extracted with 50 ml 0.5 M NaOH, at 135 W, for 3 min in intermittent power mode (power on 30 s/min). The optimized MAE achieved a 6% increase in the extraction yield (21.45 mg SDG per gram DFM) as opposed to a direct hydrolysis method (20.22 mg SDG per gram DFM). The MAE of SDG was governed by the microwave-NaOH interaction, which had a curvilinear dependence on the microwave power level, and linear dependence on the NaOH concentration. The microwave-induced effects accounted for a 10% increase in the SDG extraction yield (21.45 mg SDG per gram DFM) as opposed to a microwaveless control method (19.45 mg SDG per gram DFM). The optimized MAE method has good repeatability, a 97% recovery of the target compound; it is fast and efficient and can be used for precise quantification of SDG in flaxseed.

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Section: Introductionmentioning

confidence: 99%

Microwave-Assisted Extraction of Secoisolariciresinol Diglucoside—Method Development

Nemes

Orsat

2009

Food Bioprocess Technol

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“…Microwave-assisted extraction (MAE) is another way of accelerating the extraction of compounds from plant materials. MAE showed high efficiency in extracting essential oils [28][29][30], camptothecin alkaloids [31], and limonoids in neem trees [32]. MAE was also effective in extracting the phytochemicals in peanut [33].…”

Section: Preparation Of Botanical Samples For Initial Screening Testsmentioning

confidence: 96%

Preparation of Botanical Samples for Biomedical Research

Liu

2008

EMIDDT

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Plants are chemical storehouses, a fact which has driven countless multidisciplinary quests for bioactive compounds. As the very first step of botanical research, the whole desire is to find "hit" plants with specific bioactivities. It is logical to use some strategies that can maximize the chances of finding these "hits" with limited time and resources. In addition to selecting the right plants for screening, how the plant extracts are prepared can also influence the bioactivity screening outcomes. An extract from the same plant material can be quite different in chemical composition having different preparations. Because of the complex mixture nature of plant extracts, it is possible artifact activities may be observed. Thus confirmatory activity tests are often necessary to warrant the next laborious isolation step. A bioassay directed isolation approach may be the most efficient in identifying the bioactive compounds because of the narrowed focus at each isolation step, but a phytochemistry isolation approach is appropriate to characterize a purified bioactive extract. In fact, these two approaches can be taken intermittently whenever efficiency can be improved. Finally, use of the identified active compounds is now broader. In addition to determining a lead compound to continue a drug development path, there is an increasing interest in support for the use of botanical extracts as botanical drugs. Instead of dropping the extract after extracting the lead compound, the natural analogues representing the purified extract now have a chance to become leading compounds in the pursuit of novel therapies for metabolic syndrome and other diseases.

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“…Microwave-assisted extraction (MAE) uses microwave energy and solvents to extract target compounds from solid and semisolid samples. The highly localized temperature and pressure can cause selective migration of target compounds from the material to the surroundings at a more rapid rate and with equivalent or even higher extraction yield compared with conventional extraction [11]. With the big advantages of reducing both the extraction time and solvent consumption, this simple and consistent technique has been widely applied.…”

Section: Introductionmentioning

confidence: 99%

Development of a microwave-assisted-extraction-based method for the determination of aflatoxins B1, G1, B2, and G2 in grains and grain products

Chen

Zhang

2013

Anal Bioanal Chem

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This article describes the use of microwave-assisted extraction (MAE) as a pretreatment technique for the determination of aflatoxins B(1), G(1), B(2), and G(2) in grains and grain products. The optimal operation parameters, including extraction solvent, temperature, and time, were identified to be acetonitrile as the extraction solvent at 80 °C with 15 min of MAE. The extracts were cleaned up using solid-phase extraction followed by derivatization with trifluoroacetic acid and were determined by liquid chromatography-fluorescence detection. A Sep-Pak cartridge was chosen over Oasis HLB and Bond Elut cartridges. By the use of aflatoxin M(1) as an internal standard, relative recoveries of the aflatoxins ranged from 90.7 to 105.7 % for corn and from 88.1 to 103.4 % for wheat, with relative standard deviations between 2.5 and 8.7 %. A total of 36 samples from local markets were analyzed, and aflatoxin B(1) was found to be the predominant toxin, with concentrations ranging from 0.42 to 3.41 μg/kg.

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Influence of Operating Parameters on the Use of the Microwave-Assisted Process (MAP) for the Extraction of Azadirachtin-Related Limonoids from Neem (Azadirachta indica) under Atmospheric Pressure Conditions

Cited by 44 publications

References 14 publications

Microwave-Assisted Extraction of Secoisolariciresinol Diglucoside—Method Development

Microwave-Assisted Extraction of Secoisolariciresinol Diglucoside—Method Development

Preparation of Botanical Samples for Biomedical Research

Development of a microwave-assisted-extraction-based method for the determination of aflatoxins B1, G1, B2, and G2 in grains and grain products

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